Gait analysis plays a crucial role in assessing and monitoring the progress of individuals undergoing rehabilitation. This preliminary validation study aims to compare the performance of a new wearable system, NeuroSkin^®, equipped with embedded sensors (inertial measurement unit and pressure sensors), with the non-wearable gold standard, GAITRite^®, in assessing spatio-temporal parameters during gait. Data was collected from nine healthy participants wearing the NeuroSkin while walking on the GAITRite walkway. Temporal parameters were calculated using the pressure sensors of the NeuroSkin^® to detect heel strike (HS) and toe off (TO) on both sides. Distances were calculated using vertical hip acceleration with an inverted pendulum method. We found that the level of agreement between NeuroSkin^® and GAITRite^® measures was excellent for speed, cadence, as well as length and duration of stride and step (lower bound of intraclass correlation coefficients (ICCs) > 0.95), and moderate to excellent for stance and swing durations (ICC > 0.5). These levels of agreement are comparable to the known test–retest reliability of GAITRite^® measures. These results demonstrate the potential of NeuroSkin^® as an embedded gait assessment system for healthy subjects. As this study was conducted exclusively in healthy adults, the results are not directly generalizable to clinical populations. Thus, future studies are needed to investigate its use in patients.