The pathogenesis of cancer is intricately associated with a multitude of factors, and its precise mechanisms continue to be a central focus of rigorous scientific inquiry. Adrenomedullin (ADM), initially characterized as a potent vasodilator, has subsequently been recognized for its diverse biological functions, including roles in angiogenesis, osteogenesis, and immune modulation. Recent studies have shown that ADM, secreted by tumor cells, also plays an important role in regulating immune escape and angiogenesis in the tumor microenvironment, promoting tumor cell proliferation, resisting apoptosis, adapting to anoxic environments, and participating in the process of chemotherapy resistance. Consequently, ADM is implicated in the pathophysiology of various cancers. This review summarizes the essential functions and potential mechanisms of ADM in the occurrence and progression in cancer, and presents the associated therapeutic challenges.

Oral squamous cell carcinoma (OSCC) is a prevalent malignancy with a poor prognosis. Sestrin2 (Sesn2), a stress-inducible protein, has been implicated in various cancers, but its precise role and mechanism in OSCC remain unclear. This study investigated the molecular mechanisms of Sesn2 in OSCC. Sesn2 expression was analyzed using data from TCGA and immunohistochemical results from the HPA. Functional assays, including CCK-8, flow cytometry for cell cycle, wound healing, and Transwell assays, were performed following Sesn2 knockdown with siRNA in OSCC cell lines (CAL-27 and SAS). Underlying mechanisms were investigated by Western blotting and ELISA for MMP-2 and MMP-9 levels. Sesn2 was significantly upregulated in OSCC tissues compared to normal controls. Its knockdown markedly suppressed cell proliferation, induced G1 phase cell cycle arrest, and impaired migratory and invasive capabilities. This reduction in invasion was further confirmed by decreased levels of MMP-2 and MMP-9 upon Sesn2 knockdown. Furthermore, Sesn2 silencing induced apoptosis via Caspase-3 activation with divergent BAX/BCL-2 modulation; SAS cells exhibited elevated BAX and reduced BCL-2, whereas these proteins remained unchanged in CAL-27 cells. Mechanistically, we found that Sesn2 depletion downregulated the PI3K/AKT/mTOR pathway and reduced the phosphorylation of AKT and p38 MAPK. Our findings demonstrate that Sesn2 functions as an oncogene in OSCC, promoting tumor progression by modulating the PI3K/AKT/mTOR and MAPK signaling pathways, suggesting its potential as a therapeutic target for OSCC.

Manual dexterity is fundamental to preclinical dental training, yet opportunities for standardized early psychomotor practice remain limited. This study evaluated changes in simulator-based manual dexterity task performance following a curriculum-embedded virtual simulation course for second-year undergraduate dental students. Ninety-nine students completed identical pre- and post-training assessments using the man001 three-dimensional tunnel module over a six-week course; during the intervening training period, students practiced other, more advanced manual dexterity modules. Ninety-eight students completed an anonymous post-course questionnaire. The mean total score increased from 79.94 ± 4.54 to 86.26 ± 3.60, with a mean paired difference of 6.32 points (95% CI, 5.29–7.36; p < 0.001; Cohen’s d_z = 1.22). Leeway decreased from 10.92 ± 4.53 to 4.96 ± 2.79 (p < 0.001), and container values also decreased (p < 0.001), whereas target performance did not change significantly (p = 0.169). Overall, 92.93% of students improved their total score. Most respondents reported active engagement (94.90%), considered the course helpful (96.94%), and perceived it as clinically relevant and supportive of theory–practice integration (98.98%). The six-week course was associated with short-term changes in man001 simulator-task performance and predominantly favorable student perceptions; further research is needed to explore the retention of these changes and their transfer to broader manual dexterity and physical preclinical procedures.