This three-year field study evaluated the agronomic and physiological responses of Tritordeum to nitrogen fertilisation strategies under Mediterranean conditions using an integrated approach combining GDD-aligned phenological monitoring, UAV-based multispectral imaging, and soil analysis. Treatments included conventional urea, urea with a nitrification inhibitor (U+NI; DMPP-based), and urea with a urease inhibitor (U+UI; NBPT-based), compared to an unfertilised control. All nitrogen treatments significantly increased grain yield, reaching up to 2319 kg ha⁻¹ under the nitrification inhibitor treatment (26% higher than the control), and protein content, which peaked at 16.04% under urea. Temporal analysis revealed that urea with nitrification inhibitors consistently enhanced plant height, canopy greenness, and pigment retention during flowering to ripening stages, with NDVI and MCARI peaking under U+NI in 2025. In contrast, urea with urease inhibitor promoted greater early-season biomass and height. Soil nitrogen retention was slightly improved under both EEF treatments, with no adverse effects on pH or salinity. The strong alignment between UAV-derived indices and agronomic traits supports their use for monitoring nitrogen response. These findings demonstrate the benefits of a stage-specific fertilisation strategy, deploying urea with nitrification inhibitor early and urea with urease inhibitor during peak vegetative growth, to improve nitrogen synchrony with crop demand and support sustainable crop management in Tritordeum.

Improving nitrogen use efficiency (NUE) is critical to advancing sustainable cereal production, particularly under Mediterranean conditions where environmental pressures challenge input-intensive practises. This study evaluates NUE in Tritordeum, a climate-resilient wheat–barley hybrid, using a holistic experimental approach that integrates pre- and post-harvest soil analyses, including an electrical conductivity (EC) assessment, plant and seed nutrient profiling, and an evaluation of yield performance and nitrogen ratio dynamics. Four treatments were tested: conventional urea (T1), urea with an urease inhibitor (NBPT) (T2), urea with a nitrification inhibitor (DCD) (T3), and an unfertilised control (C). While conventional urea achieved the highest yield (1366 kg ha⁻¹), enhanced-efficiency fertilisers (EEFs) improved nutrient synchronisation and seed nutritional quality. Specifically, EEFs increased seed zinc (T2: 34.93 mg/kg), iron (T1: 33.77 mg/kg), and plant potassium (T2: 1.66%; T3: 1.61%) content, and also improved nitrogen remobilisation (elevated Nplant/Nseed ratios). EEFs also influenced soil properties, increasing organic matter (T3: 2.75%) and EC (T3: 290.78 μS/cm). These findings suggest that while EEFs may not always boost yield in the short term, they contribute to long-term soil fertility and nutrient density in grain. This study underscores the importance of synchronising nitrogen availability with Tritordeum’s phenological stages and highlights the crop’s suitability for sustainable, low-input agriculture under climate variability.