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Intravascular stent infection is a rare complication with a high morbidity and high mortality; bacteria from the hospital environment form biofilms and are often multidrug-resistant (MDR). Antimicrobial peptides (AMPs) have been considered as alternatives to bacterial infection treatment. We analyzed the formation of the bacterial biofilm on the vascular stents and also tested the inhibition of this biofilm by AMPs to be used as treatment or coating. Antimicrobial activity and antibiofilm were tested with wasp (Agelaia-MPI, Polybia-MPII, Polydim-I) and scorpion (Con10 and NDBP5.8) AMPs against Acinetobacter baumannii clinical strains. A. baumannii formed a biofilm on the vascular stent. Agelaia-MPI and Polybia-MPII inhibited biofilm formation with bacterial cell wall degradation. Coating biofilms with polyethylene glycol (PEG 400) and Agelaia-MPI reduced 90% of A. baumannii adhesion on stents. The wasp AMPs Agelaia-MPI and Polybia-MPII had better action against MDR A. baumannii adherence and biofilm formation on vascular stents, preventing its formation and treating mature biofilm when compared to the other tested peptides.
Rogério Coutinho Das Neves; Márcia Renata Mortari; Elisabeth Ferroni Schwartz; André Kipnis; Ana Paula Junqueira-Kipnis. Antimicrobial and Antibiofilm Effects of Peptides from Venom of Social Wasp and Scorpion on Multidrug-Resistant Acinetobacter baumannii. Toxins 2019, 11, 216 .
AMA StyleRogério Coutinho Das Neves, Márcia Renata Mortari, Elisabeth Ferroni Schwartz, André Kipnis, Ana Paula Junqueira-Kipnis. Antimicrobial and Antibiofilm Effects of Peptides from Venom of Social Wasp and Scorpion on Multidrug-Resistant Acinetobacter baumannii. Toxins. 2019; 11 (4):216.
Chicago/Turabian StyleRogério Coutinho Das Neves; Márcia Renata Mortari; Elisabeth Ferroni Schwartz; André Kipnis; Ana Paula Junqueira-Kipnis. 2019. "Antimicrobial and Antibiofilm Effects of Peptides from Venom of Social Wasp and Scorpion on Multidrug-Resistant Acinetobacter baumannii." Toxins 11, no. 4: 216.
Mycobacterium massiliense is a rapid growing, multidrug-resistant, non-tuberculous mycobacteria that is responsible for a wide spectrum of skin and soft tissue infections, as well as other organs, such as the lungs. Antimicrobial peptides had been described as broad-spectrum antimicrobial, chemotactic, and immunomodulator molecules. In this study we evaluated an antimicrobial peptide derived from scorpion Tityus obscurus as an anti-mycobacterial agent in vitro and in vivo. Bioinformatics analyses demonstrated that the peptide ToAP2 have a conserved region similar to several membrane proteins, as well as mouse cathelicidin. ToAP2 inhibited the growth of four M. massiliense strains (GO01, GO06, GO08, and CRM0020) at a minimal bactericidal concentration (MBC) of 200 µM. MBC concentration used to treat infected macrophages was able to inhibit 50% of the bacterial growth of all strains. ToAP2 treatment of infected mice with bacilli reduced the bacterial load in the liver, lung, and spleen, similarly to clarithromycin levels (90%). ToAP2 alone recruited monocytes (F4/80low Gr1), neutrophils (F4/80− Gr1), and eosinophils (F4/80+ Gr1+). ToAP2, together with M. massiliense infection, was able to increase F4/80low and reduce the percentage of F4/80high macrophages when compared with infected and untreated mice. ToAP2 has in vitro anti-microbial activity that is improved in vivo due to chemotactic activity.
Lázaro M. Marques-Neto; Monalisa M. Trentini; Rogério C. Das Neves; Danilo P. Resende; Victor O. Procopio; Adeliane C. Da Costa; André Kipnis; Márcia R. Mortari; Elisabeth F. Schwartz; Ana Paula Junqueira-Kipnis. Antimicrobial and Chemotactic Activity of Scorpion-Derived Peptide, ToAP2, against Mycobacterium massiliensis. Toxins 2018, 10, 219 .
AMA StyleLázaro M. Marques-Neto, Monalisa M. Trentini, Rogério C. Das Neves, Danilo P. Resende, Victor O. Procopio, Adeliane C. Da Costa, André Kipnis, Márcia R. Mortari, Elisabeth F. Schwartz, Ana Paula Junqueira-Kipnis. Antimicrobial and Chemotactic Activity of Scorpion-Derived Peptide, ToAP2, against Mycobacterium massiliensis. Toxins. 2018; 10 (6):219.
Chicago/Turabian StyleLázaro M. Marques-Neto; Monalisa M. Trentini; Rogério C. Das Neves; Danilo P. Resende; Victor O. Procopio; Adeliane C. Da Costa; André Kipnis; Márcia R. Mortari; Elisabeth F. Schwartz; Ana Paula Junqueira-Kipnis. 2018. "Antimicrobial and Chemotactic Activity of Scorpion-Derived Peptide, ToAP2, against Mycobacterium massiliensis." Toxins 10, no. 6: 219.