Nanoplastics (NPs) are a matter of widespread concern, as they are easily absorbed by a wide variety of organisms and accumulate in biological tissues. While there is evidence that nanoplastics are toxic to various organisms, few studies have investigated the mechanisms underlying the toxicities of NPs with different surface functionalizations to macrophage cells. In this study, mouse mononuclear macrophage (RAW264.7) cells were exposed to polystyrene nanoplastics (PS-NPs) with three different surface functionalizations, namely pristine polystyrene (PS), carboxyl-functionalized polystyrene (PS-COOH), and amino-functionalized polystyrene (PS-NH₂), to evaluate the cellular endocytosis, lactate dehydrogenase (LDH) release, cell viability, reactive oxygen species (ROS), mitochondrial membrane potential, apoptosis, and related gene expression. Results showed that all three PS-NPs were endocytosed into cells. However, in the concentration range of 0-100 μg/mL, PS had no effect on cell viability or apoptosis, but it slightly increased cellular ROS and decreased mitochondrial membrane potential. PS-NH₂ exhibited the highest cytotoxicity. PS-COOH and PS-NH₂ induced ROS production, altered the mitochondrial membrane potential, and caused cell apoptosis regulated by the mitochondrial apoptosis pathway. Results also showed that cell membrane damage induced by PS-NH₂ is one of the primary mechanisms of its cytotoxicity to RAW264.7 cells. The results of this study clarify the toxicities of PS-NPs with different surface functionalizations to macrophages, thereby improving the identification of immune system risks related to nanoplastics.

Groundwater is increasingly becoming a permanent and steady water source for the growth and reproduction of desert plant species due to the frequent channel cutoff events in arid inland river basins. Although it is widely acknowledged that the accessibility of groundwater has a significant impact on plant species maintaining their ecological function, little is known about the water use strategies of desert plant species to the groundwater availability in Daryaboyi Oasis, Central Tarim Basin. This study initially determined the desirable and stressing groundwater depths based on ecological and morphological parameters including UAV‐based fractional vegetation cover (FVC) images and plant growth status. Then, leaf δ¹³C values of small‐ and big‐sized plants were analyzed to reveal the water use strategies of two dominant woody species (Populus euphratica and Tamarix ramosissima) in response to the groundwater depth gradient. The changes in FVC and growth status of plants suggested that the actual groundwater depth should be kept at an appropriate range of about 2.1–4.3 m, and the minimum groundwater depth should be less than 7 m. This will ensure the protection of riparian woody plants at a normal growth state and guarantee the coexistence of both plant types. Under a desirable groundwater condition, water alternation (i.e., flooding and rising groundwater depth) was the main factor influencing the variation of plant water use efficiency. The obtained results indicated that big‐sized plants are more salt‐tolerant than small ones, and T. ramosissima has strong salt palatability than P. euphratica. With increasing groundwater depth, P. euphratica continuously decreases its growth status to maintain hydraulic efficiency in drought condition, while T. ramosissima mainly increases its water use efficiency first and decreases its growth status after then. Besides, in a drought condition, T. ramosissima has strong adaptability than P. euphratica. This study will be informative for ecological restoration and sustainable management of Daryaboyi Oasis and provides reference materials for future research programs.

Vegetation in natural desert hinterland oases is an important component of terrestrial ecosystems. Determining how desert vegetation responds to natural variability is critical for a better understanding of desertification processes and their future development. The aim of this study is to characterize the spatial distribution of vegetation in the natural desert hinterland and to reveal how different environmental factors affect vegetation changes. Taking a Taklamakan Desert hinterland oasis as our research object, we analyzed the effects of different environmental factors on desert vegetation using a time-series normalized difference vegetation index (NDVI) combined with meteorological, topographic, and hydrological data, including surface water and groundwater data. Vegetation was distributed in areas with high surface water frequency, shallow groundwater levels, relatively flat terrain, and dune basins. NDVI datasets show greening trends in oasis areas over the past 20 years. The frequency of surface water distribution influences water accessibility and effectiveness and shapes topography, thus affecting the spatial distribution pattern of vegetation. In this study, areas of high surface water frequency corresponded with vegetation distribution. The spatial distribution of groundwater depth supports the growth and development of vegetation, impacting the pattern of vegetation growth conditions. Vegetation is most widely distributed in areas where the groundwater burial depth is 3.5–4.5 m. This study provides data for restoring riparian vegetation, ecological water transfer, and sustainable development.

The spatiotemporal pattern of precipitation is significantly changing with global climate change. Snowfall is a solid phase of precipitation and an important water resource. With two gridded data sets of APHRO (Asia Precipitation‐Highly‐Resolved Observational Data Integration Towards Evaluation of Water Resources) and CN05.1, this study analyses the changes in the spatiotemporal pattern of snowfall in a snow‐dominant region of China from 1961 to 2015. The results indicate the significant increasing trend of winter snowfall in horizontal and altitude dimension in snow‐dominant regions, but the winter snowing season length shortened. For the frequency of snowfall intensity level, light, and heavy snowfall and snowstorms increased, but moderate snowfall showed no change. However, the intensity of extreme snowfall in once‐in‐a‐century was decreasing in all of the snow‐dominant regions. In the altitude dimension, the increasing trend in snow‐dominant conditions was not uniform, which may be related to change in air temperature and water vapour through the vertical atmospheric levels. The upwards trend in snowfall may be caused by the increase of atmospheric water content rather than the change of snowy weather conditions. In addition, the change values of climate indices can also contribute to snowfall increasing in snow‐dominant regions.

Understanding the water use characteristics of vegetation is crucial for guiding the rational allocation of water resources and the restoration of sustainable vegetation in natural oases in arid desert areas. To analyze the water use characteristics of Populus euphratica Oliv. and Tamarix chinensis Lour. at different stages of growth in the Daliyabuyi natural pristine oasis in northwestern China, the δ¹⁸O values of plant xylem of 15 sample trees with different sizes per species, potential water sources (i.e., river water, soil water, and groundwater), and the δ¹³C values of plant leaves of sample trees were measured in August 2019 and June 2020. The results show that the δ¹⁸O values of the xylem water of P. euphratica at different growth stages were similar, but water uptake proportions from deep soil water changed in P. euphratica trees at different growth stages between years with (2019) and without (2020) river water. In contrast, the main water source of young T. chinensis shrubs was deep soil water, and those of mature and overmature shrubs were deep soil water and groundwater in 2020. However, the plant leaf water use efficiency (WUE) of the P. euphratica and T. chinensis were higher without river water. Overall, the water uptake proportion from groundwater and the WUE for T. chinensis were higher than those for P. euphratica, and thus, T. chinensis was more dependent on groundwater. This means that vegetation types and growth stages are the essential factors to be considered in ecological restoration management, which can enhance the effectiveness of vegetation restoration strategies.