This text first dissected the relationship between average travel frequency, trip mode structure, and the characteristics of residential areas. The results showed that conducting a stratified resident travel investigation in accordance with the characteristics of residential areas will yield samples with much smaller differences and reduce the investigation sampling rate. Accordingly, a new type of resident travel investigation method was put forward based on the above ideas. The stratified sampling rate formula and the sampling rate of each layer have been derived in detail according to Probability Theory and Mathematical Statistical Methods. Finally, for the main urban area of Kunshan City in Jiangsu province, China, we discussed the reasonable values of parameters in the formulas and obtained sampling rates for travel surveys in different residential areas. Theory and case studies illustrated the operability of this method and its advantages compared to random sampling.

In this paper, mobile phone signaling data are first processed to extract information such as the trip volume and spatial distribution from the starting point to the termination point. This information is then used to identify the residential and employment locations of users. Next, multiple Thiessen polygons based on cell towers are aggregated into Traffic Analysis Zones (TAZs) to minimize differences between the actual cell tower coverage and the theoretical coverage. Then, based on TAZ cluster analysis involving transport accessibility and commuting population density, multiple stepwise regression is applied to obtain the commuting trip production rates and attraction rates for overall residential land and each subdivided housing type during the peak morning hours. The obtained commuting trip generation rates can be directly applied to local transport analysis models. This paper suggests that as information and data sharing continue, mobile phone signaling data will become increasingly important for use in future trip rate research.

Daily travel is an important means for everyone to obtain the right to development. With the development of the economy and the progress of the times, the equalization of public infrastructure has become an important concern. The accessibility and fairness of transportation have become a hot topic of research in various fields. To promote transport equity and formulate more reasonable transport planning and policies, this paper takes the Kunshan city as the research object, based on the mobile phone signaling data and the travel time consumption data from the application programming interface (API) of Gaode Map, using weighted average accessibility and the Theil index to investigate the accessibility and equity of public transport and car traffic in the Kunshan city. The study found that the accessibility of public transport is lower than that of car transport in the same research unit, but the equity of public transport is better than that of car transport, that is, the public transport is fair and the efficiency is neglected. In the same mode of transportation, equity presents a high four-week low distribution in the central urban area, and the spatial equity difference is mainly caused by the difference in accessibility levels between cell units. According to the research conclusions, it is recommended that Kunshan further optimize the spatial layout of public transportation infrastructure and adopt measures such as bus speed increase to achieve equity and efficiency and improve the competitiveness of public transportation.

Di-n-butyl phthalate (DBP) is a plasticizer commonly used in industrial production and is present in our daily life. It has been confirmed that DBP causes genitourinary malformations, especially hypospadias. However, the research of hypospadias mainly focusses on the genital tubercle in previous studies. In this study, we found DBP could affect the exocrine function of the vascular endothelium which disturb the development of genital nodules and induced hypospadias. We used cytokine array to find that vascular endothelium-derived NAP-2 may be a major abnormal secreted cytokine with biological functions. The transcriptomic sequencing analysis showed that abnormal activation of the RhoA/ROCK signaling pathway was the main reason for increased NAP-2 secretion. The expression levels of epithelial-mesenchymal transition (EMT) biomarkers and NAP-2 in hypospadias animal models were detected with Immunohistochemistry, Western blot, Immunofluorescence, and ELISA methods. The expression levels of NAP-2, RhoA/ROCK signaling pathway related proteins, reactive oxygen species (ROS) levels in HUVEC cells, EMT biomarkers and migration capacity of urothelial cells cocultured with HUVEC were measured with ELISA, flow cytometry, Western blot or Transwell assay for further cell experiments. The results showed that DBP leaded to NAP-2 oversecretion from vascular endothelium mainly rely on the activation of RhoA/ROCK signaling pathway and ROS accumulation. The RhoA/ROCK inhibitor fasudil could partially decrease ROS production, and both fasudil and N-acetyl-L-cysteine (NAC) could decrease NAP-2 secretion. Meanwhile, the oversecretion of NAP-2 from HUVEC in coculture system promoted EMT and migration capacity of urothelial cells, and TGF-β inhibitor LY219761 could block the aberrant activation of EMT process. Therefore, it could be concluded that DBP increase NAP-2 secretion from vascular endothelium by RhoA/ROCK/ROS pathway, and further promote EMT in urothelial cells through TGF-β pathway. This study provided a novel direction for studying the occurrence of hypospadias and may provide a hypospadias predictive marker in the future.

Reasonable parking charge and supply policy are essential for the regular operation of the traffic in city center. This paper develops an evaluation model for parking policies using system dynamics. A quantitative study is conducted to examine the effects of parking charge and supply policy on traffic speed. The model, which is composed of three interrelated subsystems, first summarizes the travel cost of each travel mode and then calibrates the travel choice model through the travel mode subsystem. Finally, the subsystem that evaluates the state of traffic forecasts future car speed based on bureau of public roads (BPR) function and generates new travel cost until the entire model reaches a steady state. The accuracy of the model is verified in Hangzhou Wulin business district. The related error of predicted speed is only 2.2%. The results indicate that the regular pattern of traffic speed and parking charge can be illustrated using the proposed model based on system dynamics, and the model infers that reducing the parking supply in core area will increase its congestion level and, under certain parking supply conditions, there exists an interval of possible pricing at which the service reaches a level that is fairly stable.

Rational parking allocation criteria are critical in addressing urban parking challenges and promoting the sustainable development of urban transportation. It has been common practice to reduce parking allocation for buildings according to the extent of the public transportation accessibility; however, the calculation of reduction coefficients lacks scientific rigor. This research proposes an estimation approach for reduction coefficients of parking allocation according to public transportation accessibility. First, public transportation accessibility is analyzed using Javier Gutiérrez’s weighted-average travel time model, optimizing the existing parking zoning scheme. Second, a multinomial logit model is built based on residents’ trip survey data to assess the cross-elasticity of the public transportation accessibility (travel time) with the probability of car travel. Third, the reduction in the private-car-travel probability is approximated as a parking reduction, and the reduction in the public transportation accessibility is translated to a reduction in the parking allotment, using cross-elasticity as a bridge. Finally, an empirical study is conducted on Nanjing’s central urban area, analyzing the reduction ratios within different parking zones around metro stations within specific distances and the interaction effects of these two scenarios to verify the rationality of the calculated reduction coefficients. According to the study, parking allocation standards in Zones I and II can be reduced by 10.6% and 7.5%, respectively, based on Zone III standards, while parking allocation standards within 100 m, 300 m, and 500 m of metro stations can be reduced by 17%, 12%, and 8%, respectively, based on the original standards. This paper can serve as a reference for the development of parking standard policies for public buildings.