Background: The motor response to human visual stimuli is unique and differs from the reaction to light-based visual stimuli. While laboratory-based tests offer valuable insights into athletes’ basic perceptual–motor abilities, their translation to actual sports-specific tests is limited. Methods: Following a thorough warm-up, 44 collegiate-level male soccer players (age: 24.4 ± 2.5 y, mass: 63.01 ± 7.3 kg, stature: 167.62 ± 6.3 cm) from a tertiary institution completed the following tests: Sports Vision Test (20-light proactive speed test), 40 m sprint test (split times over 5, 10, 20 and 40 m), and a live Reactive Agility Test (RAT) entailing them to sprint, change direction either towards their dominant limb or non-dominant limb in response to a live tester, and sprint again. Results: Numerous moderate correlations were seen between the RAT and various sprint distances (r > 0.3, ES > 0.3, p < 0.05). The reaction speed relationship between the light-based (SVT) and live stimuli (RAT) test yielded a weak relationship (r > 0.4, ES > 0.5, p < 0.05). Furthermore, the light-based hand–eye coordination speed did not predict acceleration or top speed, while the total RAT time did explain 10.5% of top speed (40 m). No significant differences in the SVT average and total time were found among playing positions. Conclusions: The limited correlations observed indicate that light-based reaction training alone may not be sufficient to translate to field-based reactive agility; therefore, training should integrate perceptual–cognitive and motor demands. Future research should refine laboratory-based tests by incorporating contextual elements to enhance ecological validity and further investigate the transferability of these skills from controlled settings to real-world game scenarios.

Up to 95% of a soccer match entails aerobic actions that may cause fatigue. Little is known about the effects of fatigue on the hormonal and psychological states of female players. Cortisol values (saliva sample), state anxiety (Spielberger State-trait Anxiety Inventory) and mood scores (Incredibly Short Profile of Mood States [ISP], comprising six subscales and total mood disturbances [TMD]) of 43 female players (aged 22.0±2.7 years) were taken immediately prior to and 15 min after an aerobic fatiguing test (AFT: Yo-Yo Intermittent Recovery [YYIR] test). Cortisol increased (d=0.7, p=0.007) and ISP–confusion and ISP–vigour decreased (d=0.5, p=0.01–0.02). At pre-AFT, a slight positive relationship between cortisol and anxiety-absence (r=0.3, p=0.05) was seen. TMD consistently demonstrated a strong relationship with all ISP and anxiety scores (r>0.4, p<0.01). Post-AFT results demonstrated a positive relationship between cortisol and blood lactate (r=0.3, p=0.04), between ISP–anger with maximal heart rate (r=0.3, p=0.03), ISP–anger and YYIR level as well as ISP–fatigue (r=0.4, p=0.04), and between perceived exertion rate and ISP–vigour (r=-0.4, p=0.008) as well as ISP–fatigue (r=0.3, p=0.05). Fatigue caused by prolonged activity may be a greater physiological than psychological stressor, although both may affect soccer performance. We recommend training players to increase their aerobic capacity to ensure maximal quality match-time before fatigue and its subsequent adverse physiological and psychological effects set in. Significance: The YYIR-1 test is effective in producing significant changes in both the hormonal and psychological states of female soccer players. Conditioning staff can effectively implement a mood state questionnaire to predict possible psychological stress (increase in state or trait anxiety) prior to and after a stressor. A relationship exists between cortisol and the absenteeism of anxiety prior to this fatiguing test, as well as with blood lactate thereafter. Therefore, the State-trait Anxiety Inventory questionnaire can be administered and blood lactate samples can be collected as they may display sensitive information regarding hormonal state. A positive relationship exists between the maximal heart rate achieved following an aerobic fatiguing test and various mood subscales experienced. Thus, with an increase in heart rate (as frequently observed due to fatigue), it can be assumed that an increase in perceived negative mood state might take place.

Background: Sleep hygiene protocols (SHPs) have been shown to improve sleep and stress regulation; however, the role of cortisol in shaping downstream physiological and performance adaptations remains unclear. This study primarily examined the effects of a short-term SHP on sleep duration and salivary cortisol responses across resting, pre-exercise, and post-exercise states in female collegiate soccer players and, secondarily, whether cortisol statistically mediated selected aerobic and anaerobic performance outcomes. Methods: Fourteen players (22.1 ± 3.3 y; 157.8 ± 6.0 cm; 53.5 ± 3.9 kg) completed a randomised, counterbalanced crossover study comparing habitual sleep (no sleep hygiene protocol; nSHP) with a comprehensive SHP incorporating environmental, behavioural, and educational strategies. Salivary cortisol was sampled one hour post-waking and 30 min pre- and 15 min post-exercise during standardised testing sessions. Performance outcomes included vertical jump, sprint performance (40 m and repeated sprints [RAST]), and the Yo-Yo Intermittent Recovery Test Level 1. Linear mixed-effects models assessed cortisol responses, and mediation analyses explored cortisol–performance relationships. Results: After SHP, perceived (7.87 h vs. 6.5 h; p = 0.002, ESg = 1.0) and calculated sleep duration (8.5 h vs. 6.9 h; p = 0.004, ESg = 0.95) increased significantly. Cortisol was markedly lower following SHP at selected timepoints, including before RAST (−43.05%, p = 0.006, ESg = 0.84), with additional timepoint-specific, condition-dependent differences post-anaerobic and post-aerobic exercise (Δ = 7.37 and 5.98 nmol·L⁻¹, respectively; p < 0.001). Vertical jump height demonstrated significant total (9.92 cm, p = 0.002) and direct effects (7.72 cm, p = 0.034), and peak repeated-sprint performance showed a significant direct effect (p = 0.026). Cortisol did not significantly mediate any performance outcomes (ACME p > 0.05). Conclusions: Short-term sleep hygiene is associated with increased sleep duration, timepoint-specific modulation of cortisol responses, and selected anaerobic performance benefits; however, these effects were not explained by measured cortisol responses and are unlikely to be sustained without ongoing reinforcement or support, particularly in athletic populations.

Background. Anxiety plays a major role when it comes to sports performance, not only mentally, but physically as well. Objectives. The study aimed to examine the effect of a soccer tournament on baseline anxiety [Spielberger State-trait anxiety inventory questionnaire (STAI), mood [Incredible Short POMS questionnaire (ISP)] and cortisol (saliva sampling) states. Methods. ...

Background: Sleep is a critical recovery mechanism for athletes, supporting hormonal regulation, muscle repair, and cognitive function. Dual-career athletes are particularly vulnerable to sleep disruption, which may impair performance and stress regulation. This study examined the effects of a 14-day sleep hygiene intervention protocol (SHIP) on aerobic and anaerobic performance, as well as anticipatory cortisol responses, in university-level soccer players. Methods: Thirty athletes (females: n = 14, 22.1 ± 3.3 y, 157.8 ± 6.0 cm, 53.5 ± 3.9 kg, males: n = 16, 21.5 ± 1.7 y, 167.5 ± 5.9 cm, 62.7 ± 5.4 kg) completed the Pittsburgh Sleep Quality Index (PSQI), provided pre-test salivary cortisol samples, and performed the Yo-Yo Intermittent Recovery Test Level 1 (YYIR1) and Repeated Anaerobic Sprint Test (RAST) before and after the intervention (adhering daily to 10–18 individualized sleep hygiene). Results: The SHIP significantly reduced sleep latency (p = 0.04) and increased sleep duration (p = 0.03), and PSQI scores (p < 0.001) in both sexes. Females showed marked increases in sleep duration (p = 0.002), while males showed improved latency (p = 0.07). Five behaviourally coherent clusters derived from the SHIP adherence explained a substantial proportion of variance (74.99%). Stimulant and metabolic regulation, and bedroom light and thermal environment control consistently predicted sprint and physiological outcomes (p < 0.05). Anticipatory cortisol decreased before both tests (p = 0.03–0.04). YYIR1 performance improved for the full cohort (p = 0.001). RAST times slowed slightly (p = 0.02), though fatigue index improved (p = 0.05). Conclusions: A short-term SHIP effectively enhanced sleep, reduced physiological stress, and improved key performance outcomes in collegiate athletes.