Low temperatures have adverse impacts on plant growth, developmental processes, crop productivity and food quality. It is becoming clear that Ca²⁺ signaling plays a crucial role in conferring cold tolerance in plants. However, the role of Ca²⁺ involved in cold stress response needs to be further elucidated. Recent studies have shown how the perception of cold signals regulate Ca²⁺ channels to induce Ca²⁺ transients. In addition, studies have shown how Ca²⁺ signaling and its cross-talk with nitric oxide (NO), reactive oxygen species (ROS) and mitogen-activated protein kinases (MAPKs) signaling pathways ultimately lead to establishing cold tolerance in plants. Ca²⁺ signaling also plays a key role through Ca²⁺/calmodulin-mediated Arabidopsis signal responsive 1 (AtSR1/CAMTA3) when temperatures drop rapidly. This review highlights the current status in Ca²⁺ signaling-mediated cold tolerance in plants.

Fruit ripening is a complicated development process affected by a variety of external and internal cues. It is well established that calcium treatment delays fruit ripening and senescence. However, the underlying molecular mechanisms remain unclear.

UDP-glucosyltransferase (UGT) is a key enzyme for anthocyanin biosynthesis, which by catalyzing glycosylation of anthocyanidins increases their solubility and accumulation in plants. Previously we showed that pre-harvest spray of CaCl2 enhanced anthocyanin accumulation in strawberry fruit by stimulating the expression of anthocyanin structural genes including a fruit specific FvUGT1. To further understand the regulation of anthocyanin biosynthesis, we conducted kinetic analysis of recombinant FvUGT1 on glycosylation of pelargonidin, the major anthocyanidin in strawberry fruit. At the fixed pelargonidin concentration, FvUGT1 catalyzed the sugar transfer from UDP-glucose basically following Michaelis-Menten kinetics. By contrast, at the fixed UDP-glucose concentration, pelargonidin over 150 μM exhibited marked partial substrate inhibition in an uncompetitive mode. These results suggest that the sugar acceptor at high concentration inhibits FvUGT1 activity by binding to another site in addition to the catalytic site. Furthermore, calcium/calmodulin specifically bound FvUGT1 at a site partially overlapping with the interdomain linker, and significantly relieved the substrate inhibition. In the presence of 0.1 and 0.5 μM calmodulin, V max was increased by 71.4 and 327 %, respectively. FvUGT1 activity is inhibited by anthocyanidin, the sugar acceptor substrate, and calcium/calmodulin binding to FvUGT1 enhances anthocyanin accumulation via alleviation of this substrate inhibition.

We reported previously that the preharvest treatment of broccoli microgreens with 10 mmol·L ⁻¹ calcium chloride (CaCl ₂ ) increased the yield and postharvest quality. The objective of this study was to investigate whether other calcium forms have the similar effect, in particular, after postharvest dip in calcium solution. Our results are as follows: 1) Preharvest spray without postharvest dip: Both 20 mmol·L ⁻¹ calcium lactate (Ca lactate) and calcium amino acid (Ca AA) chelate significantly improved broccoli microgreens quality and inhibited microbial populations as compared with the water-only control during storage at 5 °C for 21 days. However, they were less effective than 10 mmol·L ⁻¹ CaCl ₂ . 2) Postharvest dip without preharvest spray: The microgreens sprayed with water-only control were dipped in 0, 25, 50, or 100 mmol·L ⁻¹ Ca lactate solution containing 100 μL·L ⁻¹ chlorine immediately after harvest. During storage at 5 °C for 14 days, 50 mmol·L ⁻¹ Ca lactate dip showed the highest overall quality and lowest tissue electrolyte leakage. 3) Preharvest spray and postharvest dip: Combined preharvest 10 mmol·L ⁻¹ CaCl ₂ spray and postharvest 50 mmol·L ⁻¹ Ca lactate dip resulted in better postharvest quality than individual pre- or postharvest calcium treatments. However, the preharvest 10 mmol·L ⁻¹ CaCl ₂ spray without postharvest dip displayed a best overall visual quality and longest storage life. Our data indicate that pre- and postharvest calcium treatments have positive effect on maintaining the microgreens quality and extending shelf life. However, current postharvest dip/spinning/drying method profoundly reduces the shelf life due to mechanical damages. Technologies to optimize microgreens wash are needed to provide ready-to-eat product. Alternatively, the wash step can be avoided when the microgreens are grown under controlled settings.