The yellowfin seabream (Acanthopagrus latus) is an economically important commercial mariculture fish in China and Southeast Asia. Only a few simple sequence repeats (SSRs) of A. latus have been isolated and reported, which has hindered breeding progress. A total of 318,862 SSRs were isolated and characterized from the A. latus genome in this study. All SSRs were 9,069,670 bp in length, accounting for 1.32% of the genome. The density and frequency of SSRs were 468.40 loci/Mb and 13,323.19 bp/Mb, respectively. The major SSRs were dinucleotides (accounting for 76.92%), followed by trinucleotides (15.75%). The most abundant SSR motif was (AC)n (168,390, accounting for 53%), with the highest frequency (245.78 loci/Mb) and density (7304.18 bp/Mb). Most SSRs were located in non-coding regions, such as intergenic regions (34.54%) and introns (56.91%). SSR-containing exons were distributed into 51 gene ontology (GO) terms and significantly enriched in immunity- and growth-related pathways. A total of 217,791 SSR markers were successfully designed. Nine SSR markers were amplified in 29 A. latus individuals, and eight of them possess high polymorphism. The cross-species transferability of 33 out of the 37 tested loci were successfully amplified in Acanthopagrus schlegelii. These results lay the foundation for the molecular marker-assisted breeding and genetic information assessment of A. latus.

Cambarellus patzcuarensis is a popular aquarium freshwater crayfish in China. In this study, the mitochondrial genome of C. patzcuarensis was sequenced using Illumina HiSeq. The coding mitogenome of C. patzcuarensis is 15,000 bp in length, consisting 13 protein-coding genes (PCGs), 22 transfer RNAs, and two ribosomal RNAs. The A + T content was 70.17%. Ten PCGs had ATN as the start codon. Three PCGs were stopped with incomplete stop codon. The phylogenetic analysis shown that C. patzcuarensis was grouped with other Procambarus species. These results are useful for understanding the phylogenetic relationships of Cambaridae crayfish.