Two field experiments were carried out at the Experimental Farm (GhazalaVillage), Faculty of Agriculture, Zagazig University, Sharkia Governorate, Egypt, during2015/2016 and 2016/2017 seasons to study the influence of the bio-fertilizer Azotobacter(without and with inoculation) and four N levels (0, 40, 80 and 120 kg N/fad.) on nitrogenuse efficiency (NUE), yield and its attributes of two bread wheat cultivars (Gemmeiza11and Misr2). Results of combined analysis revealed that Misr2 cultivar produced the highestnumber of spikes per square meter, grain and biological yields compared to Gemmeiza11 whichwas superior in plant height, spike length, spike grain weight, thousand grain weight and harvestindex, as well it was higher in NUE. Inoculating wheat grains with Azotobacter chroococcun (incommercial form Biogein) before sowing significantly increased studied traits i.e. Plant height,spike length, spike number/m2, grain weight/spike, 1000-grain weight, grain and biologicalyields/fad., and harvest index, but in contrary NUE decreased by Azotobacter inoculationduring the two seasons and their combined analysis. Results of combined analysis alsoindicated that increasing N application level up to 120 kg N/fad., significantly increased each ofplant height, spike length, spike number/m2, grain weight/ spike, grain and biological yields/fad., and decreased NUE that responded to N addition up to 80 kg N/fad. Significantinteractions between the studied factors indicated that the addition of Azotobacter increasedthe response of wheat cultivars to the increase of N level particularly. According to theresults of the interactions between cultivars and Azotobacter, cultivars and N-fertilizer levelsand between Azotobacter and N levels, wheat cultivar Misr2 should be fertilized withAzotobacter and 120 kg N/fad., to produce the highest yield. Moreover, the results showedthe significant interaction effect between bio and N fertilizer levels on NUE where theefficiency of N use was higher with Azotobacter inoculation and 80 kg N/fad., incomparison to control and 120 kg N/fad.