Clinacanthus nutans (Burm.f.) Lindau is a Southeast Asian medicinal plant traditionally used for treating skin inflammation and infections. This study evaluated its wound-healing potential through anti-inflammatory, cytoprotective, and antiviral mechanisms. HPLC-DAD analysis identified schaftoside as the major flavonoid in the 95% ethanolic leaf extract. In the lipopolysaccharide (LPS)-stimulated murine macrophage cell line (RAW 264.7), both C. nutans extract (5 and 50 μg/mL) and its flavonoid schaftoside (5 and 20 μg/mL) significantly downregulated the expression of pro-inflammatory genes, including cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), and prostaglandin E₂ (PGE₂), under both pre-treatment and post-treatment conditions. ELISA confirmed dose-dependent inhibition of human COX-2 enzymatic activity, reaching up to 99.3% with the extract and 86.9% with schaftoside. In the endothelial cell models (CCL-209), the extract exhibited low cytotoxicity and effectively protected cells from LPS-induced apoptosis, preserving vascular integrity critical to tissue regeneration. Antiviral assays demonstrated suppression of HSV-2 replication, particularly during early infection, which may help prevent infection-related delays in wound healing. Collectively, these findings suggest that C. nutans and schaftoside promote wound repair by attenuating inflammatory responses, supporting endothelial survival, and controlling viral reactivation. These multifunctional properties highlight their potential as natural therapeutic agents for enhancing wound-healing outcomes.

Clinacanthus nutans (Burm.f.) Lindau is a medicinal plant known for its antioxidant, anti–inflammatory, and antiviral properties. Drought is a major abiotic stress affecting plant physiology and secondary metabolite biosynthesis. This study investigated the physiological and biochemical responses of C. nutans under drought stress. Relative water content declined with prolonged drought, while hydrogen peroxide and proline levels increased, indicating oxidative and osmotic stress. Antioxidant activities (DPPH and ABTS) peaked at days 4–8 and showed positive correlations with phenolic and flavonoid contents and triterpenoids, particularly schaftoside and lupeol. Molecular docking supported the bioactivity of drought–induced metabolites, with schaftoside and lupeol showing favorable interactions with inflammation–related targets. Multivariate analysis revealed that short–term drought enhanced stress tolerance and secondary metabolite accumulation, whereas prolonged stress reduced biosynthetic capacity and survival. These findings suggest that controlled drought exposure can enhance bioactive compound levels in C. nutans, supporting its potential for drought–adaptive cultivation for medicinal use.

ISHS III Asia Pacific Symposium on Postharvest Research, Education and Extension: APS2014 A technique to detect cell damage due to chilling injury for fruits and vegetables using Trypan blue and Evans blue

ISHS IV Asia Symposium on Quality Management in Postharvest Systems Degradation of pesticide residue in 'Mahajanaka' mango fruit during storage at different conditions

ISHS VIII International Postharvest Symposium: Enhancing Supply Chain and Consumer Benefits - Ethical and Technological Issues Cell death due to chilling injury of mango cultivars 'Nam Dok Mai No.4', 'Nam Dok Mai Si Thong' and 'Mahachanok' during low temperature storage