Cashmere fineness is one of the important factors determining cashmere quality; however, our understanding of the regulation of cashmere fineness at the cellular level is limited. Here, we used single-cell RNA sequencing and computational models to identify 13 skin cell types in Liaoning cashmere goats. We also analyzed the molecular changes in the development process by cell trajectory analysis and revealed the maturation process in the gene expression profile in Liaoning cashmere goats. Weighted gene co-expression network analysis explored hub genes in cell clusters related to cashmere formation. Secondary hair follicle dermal papilla cells (SDPCs) play an important role in the growth and density of cashmere. ACTA2, a marker gene of SDPCs, was selected for immunofluorescence (IF) and Western blot (WB) verification. Our results indicate that ACTA2 is mainly expressed in SDPCs, and WB results show different expression levels. COL1A1 is a highly expressed gene in SDPCs, which was verified by IF and WB. We then selected CXCL8 of SDPCs to verify and prove the differential expression in the coarse and fine types of Liaoning cashmere goats. Therefore, the CXCL8 gene may regulate cashmere fineness. These genes may be involved in regulating the fineness of cashmere in goat SDPCs; our research provides new insights into the mechanism of cashmere growth and fineness regulation by cells.

This study determined the effects of rumen-protected methionine (RPM) supplementation on the growth performance, nutrient digestibility, nitrogen (N) utilisation and plasma amino acid profiles of Liaoning cashmere goats during cashmere fibre growth. Twenty-four yearling male cashmere goats (body weight: 35.41 ± 1.13 kg) were randomly assigned to four dietary treatments: a corn–soybean meal basal diet deficient in methionine (negative control, NC) and a basal diet supplemented with 1, 2 and 3 g/kg of RPM. The RPM supplementation quadratically increased the average daily gain (ADG) and decreased the feed to gain ratio (p = 0.001) without affecting the final body weight and dry matter intake. In particular, compared to NC, 2 g/kg RPM supplementation increased the ADG by 35 g/d (p < 0.001) and resulted in the lowest feed to gain ratio (p < 0.001). RPM increased the apparent total tract digestibility of N and decreased the faecal N levels, both in a linear fashion (p = 0.005). Urinary N levels did not have an effect, but the N retention levels increased linearly with PRM (p = 0.032). Moreover, the RPM decreased the plasma urea N levels (p < 0.001) and increased the plasma Met levels quadratically (p < 0.001). In conclusion, RPM supplementation in the diet of cashmere goats can enhance the utilisation of N and improve ADG during the cashmere fibre growing period, and 2 g/kg of RPM in the diet is suggested.

This study was conducted to compare the effects of hydrolysable versus condensed tannins on growth performance, nutrient digestion, and rumen fermentation in goats. A total of 27 Liaoning cashmere goats with similar initial body weights were randomly distributed into three groups: a basal diet, a basal diet +0.5% tannic acid (hydrolysable tannin, HT), or a basal diet +0.5% quebracho tannin (condensed tannin, CT), respectively. Compared to the control group, HT showed a decreased (p < 0.05) feed intake, while CT showed an increased (p < 0.05) feed intake and body weight gain. Digestibility of dry matter, crude protein, neutral detergent fiber, and acid detergent fiber did not differ (p > 0.05) among groups. The HT group showed lower pH and acetic acid concentration in the rumen (p < 0.05), while the CT group showed a decrease (p < 0.05) in the abundances of Verrucomicrobia and Methanobrevibacter. Supplementation of CT decreased (p < 0.05) the levels of dihydrouracil, xanthosine, alpha-D-mannose, and L-tryptophan, while HT increased (p < 0.05) these metabolites. In conclusion, this study suggested that responses of ruminal microbiota and metabolite profiles to the type of tannins varied, and CT displayed a more positive effect on the growth performance of goats than HT when supplemented at the same level in the diets.

In this study, microsatellite loci in geese were initially screened, after which a multiplex PCR system was constructed and the genetic diversity and genetic structure of 40 samples from each of the three Huoyan goose populations in Liaoning were analyzed using the STR typing test. The results showed that 12 microsatellite loci were screened, and one quadruple PCR system, two triple PCR systems and one double PCR system were constructed with consistent and reliable reproducibility. A total of 90 alleles were detected in 120 samples, the number of alleles(Na), observed heterozygosity (Ho), expected heterozygosity (He) and polymorphic information content(PIC) of the three populations ranged from 5.083 to 6.083, from 0.149 to 0.184, from 0.555 to 0.577 and from 0.503 to 0.512.respectively, the populations present an unbalanced state, and the genetic diversity of three populations was highly polymorphic and highly inbred. The results of Principal Coordinates Analysis (PCoA) and genetic structure analysis showed that the genetic backgrounds of the three populations of Huoyan geese were similar, with close affinities and frequent gene exchanges.