The aim of this study was to provide information on post-harvest losses (PHLs) along the main value-added chains in the soybean industry, and on farmers’ strategies for reducing these losses in Benin. Data were collected using a subjective method (questionnaires) from 152 direct actors in the soybean value chain and an objective method (technological monitoring using technological monitoring sheets) from 27 processing units using a two-stage sampling technique in eighteen of the country’s municipalities. The data were analyzed using descriptive statistics. The results revealed that post-harvest losses in soybean value chains ranged from 0.03% to 7.98%. The highest loss percentages were 7.98% (production), 10.0% (processing), and 4.27% (marketing), and were obtained during the subjective measurements. The link most affected by PHL was the production link. These losses were mainly observed during threshing, winnowing, sorting, and harvesting operations. The main causes of these losses were poor mechanization and a lack of skills for post-harvest operations. The main strategies developed by players to cope with this situation remained traditional (timely harvesting, use of appropriate equipment, and good storage practices). However, additional efforts in terms of infrastructure, adapted subsidies/credits, and recruitment of specialists are needed to mechanize the operations most sensitive to losses and to reinforce the capacities of agricultural actors in order to considerably reduce post-harvest losses.