The results of SHRIMP U-Pb ages and in situ Hf isotope of zircons from three granites in the southern Manzhouli region of northeast China, provide new data to understand the subduction process of Mongol-Okhotsk Plate beneath the Erguna massif. SHRIMP U-Pb zircon geochronology results yield an age of 265.5 Ma (middle Permian) for fine-grained monzogranite. Rocks from the Early–Middle Triassic are mainly granodiorite (247.4 ± 4.6 and 249.3 ± 4.9 Ma), the granites are with SiO₂ = 60.0–77.4 wt.%, Al₂O₃ = 12.3–16.8wt.% and Na₂O/K₂O = 0.7–1.9. Chemically, they are metaluminous to peraluminous and belong to the high-K calc-alkaline series. Enrichments in the large ion lithophile elements (e.g., Rb, Ba, and K) and depletions in the high field strength elements (e.g., Nb, Ta, and Ti) are typical for these rock types. The monzogranite (~265 Ma) and granodiorite (~247 Ma) contain zircons with εHf(t) values of 6.3–8.5 and 5.1–7.9, yielding T_DM2 model ages of 888–752 and 958–774 Ma, respectively. These geochemical and zircon Hf isotopic data indicate that primary magmas for Middle Permian–Early Triassic granites crystallized from primary magmas generated by Neoproterozoic crustal materials, formed in an active continental margin setting. The andesite of the Gegenaobao formation is similar with the Izu–Bonin–Mariana arc, relating to subduction initiation. Based on the characteristics of exposed rocks and zircon U-Pb ages of andesite and granitoid rocks in the study area, we conclude the onset subduction of Mongol-Okhotsk Plate beneath the Erguna massif may occur at early-middle Permian.