Background: Urinary tract infections (UTIs) acquired in hospitals, particularly among patients in intensive care units (ICUs), are prevalent and represent a significant clinical issue as they are associated with increased patient morbidity, prolonged hospital stays, elevated healthcare costs, and antimicrobial resistance development. Methods: This study was conducted in the ICU of the University Hospital in Wrocław, Poland, from January 1, 2020, to June 30, 2024, and included 2,751 patients. The aim was to evaluate the incidence, epidemiological, and microbiological characteristics, mortality rates, and prevention strategies of UTIs during and after COVID-19 pandemic. Results: Catheter-Associated Urinary Tract Infection (CAUTI) (48 hours after admission) was recorded in 243/2751 (8.83%) patients, whereas UTI at admission was found in 63/2751 (2.3%). The mean CAUTI incidence rate (per 1,000 patient-days) was 6.99, 95% CI (6.13-7.85), whereas the mean CAUTI incidence density (per 1,000 urinary catheter days) was 7.04, 95% CI (6.18-7.91). CAUTI was significantly more frequent in females (12.32%) than in males (6.85%), p = 0.0000008, and in internal-medical patients (14.07%) compared to surgical patients (6.45%), p < 0.000001. The mean CAUTI density during the pandemic equaled 8.03, 95% CI (6.46-9.60) and was greater than in the post-pandemic period 6.25, 95% CI (5.34-7.17), p = 0.051. No statistically significant difference was observed in CAUTI incidence between COVID-positive 27/264 (10.23%) and COVID-negative 83/904 (9.18%) patients, p = 0.942. The most frequently identified pathogen in CAUTI was Klebsiella pneumoniae ESBL/MBL, whereas in UTI at admission, it was Escherichia coli. The percentage of alert pathogens among CAUTI etiological factors was significantly higher during the pandemic 72/116 (62.07%), compared to the post-pandemic period 62/143 (43.36%), p = 0.002. There was no statistically significant difference in mortality between CAUTI patients 56/207 (27.05%) and patients without CAUTI 810/2,544 (31.84%), p = 0.119. Conclusion: Although there were no statistically significant differences observed in CAUTI incidence between COVID-positive and COVID-negative patients, CAUTI remains a significant challenge in the ICU setting, with particularly elevated risks among female and internal-medical patients. Consistent monitoring of CAUTI, implementation, evaluation of preventive measures, and ongoing assessment are essential for improving clinical outcomes.

Background/Objectives: The COVID-19 pandemic significantly disrupted healthcare systems worldwide, leading to increased healthcare-associated infection rates, particularly in the intensive care unit (ICU) setting. Little is known about the evolution of this phenomenon in subsequent years. Methods: This retrospective analysis of prospectively collected data (January 2020–December 2024) examined central line-associated bloodstream infections (CLABSI) in the Wroclaw Medical University hospital’s ICU during and after the COVID-19 pandemic. Results: Ninety CLABSI cases were observed in 3149 ICU patients across 39,837 patient-days and 36,038 central-vascular-catheter-days (CVC-D). The mean CLABSI frequency was 2.97 per 100 admissions, with an incidence density of 2.49 per 1000 CVC-D. CLABSI occurred more frequently in males than in females (3.51% vs. 1.69%, p = 0.003) and in patients with concomitant SARS-CoV-2 infection than in individuals without such coinfection (6.06% vs. 1.88%, p = 0.00037). Microbiological analysis revealed Staphylococcus epidermidis as the most frequent etiological factor of CLABSI (33.3%). Alert pathogens constituted 34.26% of all CLABSI etiological factors, with higher prevalence during the pandemic than afterward (51.16% vs. 23.08%, p = 0.005437). Patients with CLABSI had significantly longer ICU stays (53.57 vs. 11.62 days, p = 0.001). After adjusting for immortal time bias using matched cohort analysis, CLABSI was not associated with increased mortality (p = 0.735). The overall compliance level of adherence to CLABSI prevention measures was 86.9%, with no statistically significant difference between the pandemic and post-pandemic periods, p = 0.417. The study did not systematically collect data on catheter types, insertion sites, or clinical circumstances (emergency vs. elective), which are known risk factors that may have influenced the observed CLABSI incidence rates. Conclusions: Despite increased patient volume post-pandemic, CLABSI metrics remained stable, possibly due to the successful adaptation of infection prevention protocols. However, interpretation of incidence data should consider unmeasured confounding factors. These findings address knowledge gaps regarding how the pandemic affected CLABSI epidemiology and antimicrobial resistance patterns, with implications for infection control practices during future healthcare crises.