This paper proposes an automated system integrating infrared thermography (IRT) and computer vision for air leak detection and localization in end-of-line (EOL) testing stations. This system consists of (1) a leak tester for detection and quantification of leaks, (2) an infrared camera for real-time thermal image acquisition; and (3) an algorithm for automatic leak localization. The python-based algorithm acquires thermal frames from the camera’s streaming video, identifies potential leak regions by selecting a region of interest, mitigates environmental interferences via image processing, and pinpoints leaks by employing pixel intensity thresholding. A closed circuit with an embedded leak system simulated relevant leakage scenarios, varying leak apertures (ranging from 0.25 to 3 mm), and camera–leak system distances (0.2 and 1 m). Results confirmed that (1) the leak tester effectively detected and quantified leaks, with larger apertures generating higher leak rates; (2) the IRT performance was highly dependent on leak aperture and camera–leak system distance, confirming that shorter distances improve localization accuracy; and (3) the algorithm localized all leaks in both lab and industrial environments, regardless of the camera–leak system distance, mostly achieving accuracies higher than 0.7. Overall, the combined system demonstrated great potential for long-term implementation in EOL leakage stations in the manufacturing sector, offering an effective and cost-effective alternative for manual inspections.