The Chinese horned toads, Boulenophrys boettgeri (Boulenger, 1899) and Boulenophrys kuatunensis (Pope, 1929), are two captivating species within the family Megophryidae, which inhabit the mountainous streams in the Eastern of China. In this study, two new complete mitochondrial genomes of B. boettgeri and B. kuatunensis were sequenced, assembled, and annotated using next-generation sequencing. The length of mitochondrial genomes of B. boettgeri and B. kuatunensis was 16,597 and 17,921 bp, respectively, with both containing 13 protein coding genes, 22 tRNA genes, two rRNA genes, and one putative control region. Phylogenetic relationships based on protein-coding mitochondrial genes showed that the two Boulenophrys species formed a cluster with other Boulenophrys species. The two new sequences provide valuable insights into the mitochondrial genomes of these two species, offering important data for understanding the phylogenetic relationships of the genus Boulenophrys.

Elevational gradients provide an excellent opportunity to assess biodiversity patterns and community structure. Previous studies mainly focus on higher elevations or are limited to small areas in mountainous regions. Little information can be found on amphibian biodiversity in middle- and low-elevational areas, hence our study was devoted to filling up the current gaps in these research areas. To understand the variability of biodiversity of amphibian species in the Fujian Junzifeng National Nature Reserve in eastern China, our study included taxonomic and phylogenetic components to describe the various patterns of regional and elevational distribution. The results showed that (1) most of the taxonomic and phylogenetic diversity metrics were correlated; with regard to the surveyed area, Faith’s phylogenetic diversity index (PD) and net relatedness index (NRI) were positively correlated with the Shannon–Wiener index (H’), Margalef index (DMG), and species richness (S), while negatively with the Pielou index; whereas for elevation, only the Pielou index was positively correlated with the nearest taxon index (NTI), but negatively with other indices; (2) taxonomic and phylogenetic diversities did not differ among the three survey locations but differed significantly along the elevational gradient; Simpson index, H’, S, and DMG had a hump-shaped relationship with elevations, and PD decreased gradually with the increase in elevation, whereas NRI and NTI sharply increased at the elevation above 900 m; (3) the species range size and the corresponding midpoint of amphibians were affected by a strong phylogenetic signal, which supports the elevational Rapoport’s rule upon removal of Pachytriton brevipes and Boulenophrys sanmingensis from the study.

The intestinal microbiota regulates host metabolic homeostasis through production of bioactive microbial metabolites. These microorganisms facilitate digestion, enhance immune function, maintain osmoregulation, and support physiological balance via these bioactive compounds, thereby enhancing environmental adaptation. Our study investigated intestinal microbiota–liver metabolic interactions in Leptobrachium liui using 16S rRNA gene sequencing and non-targeted liquid chromatography–tandem mass spectrometry metabolomics. Key findings include (1) comparable alpha diversity but distinct microbial community structures between the small intestine (SI) and large intestine (LI), with the SI dominated by Enterobacteriaceae (72.14%) and the LI by Chitinophagaceae (55.16%); (2) segment-specific microbe–metabolite correlations, with predominantly positive correlations in the SI and complex patterns in the LI involving fatty acids, amino acids, and energy metabolites; and (3) significant correlations between specific bacterial families (Aeromonadaceae, Enterobacteriaceae, Chitinophagaceae) and hepatic metabolites related to fatty acid metabolism, amino acid synthesis, and energy pathways, indicating potential gut–liver axis associations. These findings provide insights into amphibian intestinal microbiota–hepatic metabolite associations and may inform future studies of host–microbe interactions.

Advertisement calls play a crucial role in anuran species recognition and mate selection, yet acoustic characteristics of Zhangixalus lishuiensis remain undescribed. This study presents the first detailed acoustic analysis of advertisement calls in Zhangixalus lishuiensis from Lishui, Zhejiang Province, China. Based on recordings from 13 males collected in the field during the breeding season, we documented a hierarchical call structure consisting of three note types (Initial Note, Middle Note, and Short Note) organized into two note groups (Note Group 1 (NG1) and Note Group 2). Four structural variants of NG1 were identified, revealing intra-individual acoustic diversity within the population. Compared to the closely related Z. zhoukaiyae from the Dabie Mountains population, male Z. lishuiensis produced calls with significantly longer temporal parameters but lower dominant frequency, despite overall structural similarity, indicating acoustic divergence between these taxa. Comparative analysis across eleven Zhangixalus species revealed substantial variation in call durationand dominant frequency. These findings enrich the bioacoustic characterization of Zhangixalus and establish a foundation for acoustic-based species identification, monitoring, and taxonomic studies in this genus.

Hongze Lake is China’s fourth largest freshwater lake and is also an important habitat for hundreds of thousands of migratory birds on the East Asian-Australian Flyway (EAAF). Sihong Hongze Lake Wetlands National Nature Reserve is located on the northwest of Hongze Lake, Sihong County, Jiangsu Province. The Reserve is a protected large area of natural lake wetlands, marsh wetlands and riverine wetlands and used as a stopover and wintering habitats for migratory birds. Previous studies have conducted bird diversity and temporal-spatial variation in this Reserve, but only for species of Anseriformes. There is still a lack of a comprehensive dataset on the number of bird species and individuals in this Reserve throughout the year. Our study was conducted from July 2020 to June 2021 to observe bird species composition and individual numbers at Sihong Hongze Lake Wetlands National Nature Reserve and provides an occurrence dataset with detailed species and geographic information. This occurrence dataset is the first public record of birds in Sihong Hongze Lake Wetlands National Nature Reserve for a whole year, which includes the taxonomic information, location information, number, investigation date and endangered level for each species. All data have been published on GBIF.

Hongze Lake is China’s fourth largest freshwater lake and is also an important habitat for hundreds of thousands of migratory birds on the East Asian-Australian Flyway (EAAF). Sihong Hongze Lake Wetlands National Nature Reserve is located on the northwest of Hongze Lake, Sihong County, Jiangsu Province. The Reserve is a protected large area of natural lake wetlands, marsh wetlands and riverine wetlands and used as a stopover and wintering habitats for migratory birds. Previous studies have conducted bird diversity and temporal-spatial variation in this Reserve, but only for species of Anseriformes. There is still a lack of a comprehensive dataset on the number of bird species and individuals in this Reserve throughout the year. Our study was conducted from July 2020 to June 2021 to observe bird species composition and individual numbers at Sihong Hongze Lake Wetlands National Nature Reserve and provides an occurrence dataset with detailed species and geographic information. This occurrence dataset is the first public record of birds in Sihong Hongze Lake Wetlands National Nature Reserve for a whole year, which includes the taxonomic information, location information, number, investigation date and endangered level for each species. All data have been published on GBIF.