Climate change has potential effects on global biodiversity by shifting the optimal distribution of terrestrial organisms, particularly species with narrow distribution ranges. Goniurosaurus lichtenfelderi, a forest-dwelling lizard, was first found in both islands and mainland of northern Vietnam and subsequently in southern China. The narrowly distributed species was assessed in the IUCN Red List as Vulnerable and recently listed in CITES Appendix II and the Vietnam Governmental Decree in 2019 due to over-exploitation for the international pet trade. In this study, we applied the maximum entropy algorithm within the Maxent software using climatic and vegetation cover data to identify the potential distribution range of G. lichtenfelderi. Additionally, we used this approach to assess future climate effects on distribution alterations under different scenarios. Overall, our model predicted that the potential distribution range of G. lichtenfelderi will decline significantly under future scenarios, even totally disappear in the BCC-CSM 1-1 – RCP 8.5 scenario by 2070 in the study area. Potential distribution is expected to be shrunk and/ or altered towards higher latitudes within the next decades. The forecasted maps provide useful guidelines to implement conservation strategies to mitigate both climate change and further negative anthropogenic effects. In the context of the potentially severe effects of climate change, we propose the border areas between China and Vietnam, Yen Tu mountain range, Bai Tu Long National Park, and their surroundings as the core refugia of G. lichtenfelderi for priority conservation approaches.

A new species of the genus Tylototriton is described from Ngoc Linh Mountain, Kon Tum Province, in the Central Highlands of Vietnam based on integrative taxonomy, namely by combining molecular and morphological evidence. Tylototriton ngoclinhensis sp. nov. differs from all other congeners based on morphological data, allopatric distribution, and molecular divergence. In terms of genetic divergence, Tylototriton ngoclinhensis sp. nov. distinctly differs from the sister species T. panhai (6.77%) and from T. ngarsuensis (12.36%) based on the mitochondrial NADH dehydrogenase subunit 2 (ND2) gene. Tylototriton ngoclinhensis sp. nov. is a moderate sized and robust salamander species with large cephalic edges, parotoids, and vertebral ridge orange in coloration. The new taxon differs from its congeners by a combination of the following morphological characteristics: size medium (SVL 60.8–66.5 mm, TL 57.6–61.8 mm in males, and SVL 72.5–75.6 mm, TL 62.9–67.9 mm in females); head longer than wide; parotoids very prominent and enlarged, projecting backwards; tail length shorter than snout-vent length; vertebral ridge large, high and glandular in appearance; 14 large and distinct dorsolateral glandular warts; gular fold present; tips of fore and hind limbs overlapping when adpressed along the body; tips of fingers reaching between eye and nostril when foreleg is laid forward; dorsal surface and lateral sides of the head, upper and lower lips, dorsolateral glandular warts, vertebral ridge, the peripheral area of the cloaca and the ventral edge of the tail orange in coloration; the presence of a distinct black line extending from the posterior end of the eye towards the shoulder. Tylototriton ngoclinhensis sp. nov. is restricted to evergreen montane forests near water bodies on Ngoc Linh Mountain. We suggest that the new species should be classified as Endangered (EN) in the IUCN Red List. This new important discovery represents the eighth Tylototriton taxon described from Vietnam, and at the same time constitutes the southernmost distributional record for the whole genus in Asia.

Amolops splendissimus Orlov and Ho, 2007 and A. caelumnoctis Rao and Wilkinson, 2007 were described almost simultaneously from either side of the China-Vietnam border. The two species share a strong morphological resemblance, and their taxonomic distinctiveness has been questioned, yet no one has confirmed the taxonomic relationship and status between the two taxa. To resolve this taxonomic issue, we collected additional topotypic and near-topotypic specimens of A. splendissimus and A. caelumnoctis from both China (A. caelumnoctis: Wenshan County, Yunnan Province; type locality Luchun County, Yunnan Province), and Vietnam (A. splendissimus: Tam Duong District, Lai Chau Province; type locality Mount Ky Quan San, Bat Xat, Lao Cai Province). Molecular analysis based on a 16S rRNA fragment revealed minimal genetic divergences between the two taxa (0.0%–0.4% uncorrected p-distance), and both species are closely related to A. viridimaculatus (2.1%–2.3%) and A. medogensis (3.5%–3.7%). Morphological comparisons between the newly collected specimens and the original descriptions of both species further support the lack of distinctiveness of the two species, hence, we conclude that A. caelumnoctis is a junior synonym of A. splendissimus.

We describe a new species of Theloderma from northeastern Vietnam based on morphological differences and molecular divergence. Theloderma khoii sp. nov. is distinguishable from its congeners on the basis of a combination of the following characters: large size, SVL 52.1 mm in male, 59.4 mm in female; head length and width equal; vomerine teeth present; snout pointed and truncated, eye large, ED 4.7 mm in male, 5.6 mm in female, spinules on upper eyelid; tibiotarsal articulation reaches to the posterior border of the eye or the tip of the snout; dorsal skin very rough with large irregular gland ridges and warts, ventral surface of body granular; tips of all digits dilated but all considerably smaller than tympanum; dorsal surface mossy green or olive mottled with dark magenta. The distribution of the new species is unknown but probably extends into adjacent high elevation forested areas in Ha Giang Province, Vietnam and in Yunnan Province, China with an extent of occurrence of only < 1000 km² and continuing decline in the quality of its habitat due to deforestation. Thus, we suggest the species should be considered Endangered following IUCN’s Red List categories.

Anthropogenic pressures have caused a substantial decline of global biodiversity and have been further reported to strongly affect the ecological performance of species in their habitat, especially reptiles. Understanding the ecology of species and how species respond to habitat alterations is basic knowledge needed to develop conservation programmes and address issues of biodiversity loss. All five species of tiger geckos (Goniurosaurus) in Vietnam are known to be threatened by extinction due to anthropogenic impacts such as habitat degradation and harvesting for the international pet trade. However, conservation actions have only been initiated for the better-studied species. This study provides detailed basic data on microhabitat use of two allopatric sister species, namely Goniurosaurus huuliensis and G. luii. In total, 145 geckos (including 59 records of G. huuliensis and 86 records of G. luii) were observed during field surveys. All Goniurosaurus individuals were mostly recorded in the forest on karst formations, covered with evergreen broad-leaved woody trees, intermixed with ferns, shrubs and vines. Microhabitats of the two species were relatively similar in other traits, such as high vegetation coverage, high humidity, stable ambient temperature and dry-rock substrates. A multiple factor analysis supported that the ecological niche spaces of the two species highly overlap, even though their distribution ranges are geographically separated. We further found no intraspecific niche segregation in both species. The present data provide baseline knowledge for both in situ and ex situ conservation measures to protect species in the genus Goniurosaurus.

A new species of the genus Vietnamophryne is described from Vietnam on the basis of two specimens collected from Tuyen Quang Province, Northeastern Vietnam. The new species is morphologically most similar to Vietnamophryne occidentalis from Thailand, however, it differs from the latter by having large black blotches in the lower jaw region, and a yellow-orange chest and belly. The genetic distance between the new species and other Vietnamophryne taxa is > 2.13% (16S mtDNA gene fragment). Vietnamophryne aurantifusca sp. nov. can be distinguished from all other species of Vietnamophryne by a combination of the following morphological characteristics: Size medium (SVL 17.6–18.2 mm in males); head wider than long; tympanum medium; finger I longer than half of finger II; dorsal skin relatively smooth with some round nodules, concentrated in the middle of the back, arranged along the length of the back, with a prominent ridge along the spine; Dorsum orangish-brown entirely and paler on margin of back with a small brownish ridge along the spine; sides brownish with creamy patches and orange spots; ventral surface orange, with grey marbling, most intense on the throat, ventral side of arms and thighs, and ventral surfaces of limbs dark grey with some orange spots.