With the increasing complexity of mine ventilation networks, the difficulty of regulating ventilation systems has significantly increased. Lagging regulatory responses are prone to causing problems such as airflow turbulence and insufficient air supply in air-required areas, which seriously threaten the safety of underground operations. To address this challenge, this paper introduces the MOBWO algorithm into the field of ventilation system air volume optimization and proposes a mine air volume optimization and regulation method based on MOBWO. This paper constructs a multi-objective air volume optimization model with the total power of ventilators and the complexity of air pressure regulation as the optimization objectives. Using indicators such as GD and IGD, it compares the performance of the MOBWO algorithm with mainstream optimization algorithms such as NSGA-II and MOPSO and verifies the practicality of the optimization method with the case of the Jinhua Palace Mine. The results show that the MOBWO algorithm has significant advantages over other algorithms in terms of convergence and distribution performance. When applied to the Jinhua Palace Mine, the air volume optimization and regulation using MOBWO can reduce the power of ventilators by 10.3–21.1% compared with that before optimization while reducing the complexity of air volume regulation and the time loss during air volume regulation. This method not only reduces the energy consumption of ventilators but also shortens the regulation timeliness of the ventilation system, which is of great significance for reducing the probability of accidents and ensuring the safety of personnel’s lives and property.