Thirteen is the charm in anaphylaxis: Immunoglobulin E (IgE) is a type of antibody associated with allergies and response to parasites such as worms. When high-affinity, allergen-specific IgE binds its target, it can cross-link receptors on mast cells that induce anaphylaxis. It remains unclear, however, how B cells are instructed to generate high-affinity IgE. Gowthaman et al. discovered a subset of T follicular helper cells (T _FH 13) that direct B cells to do just that. T _FH 13 cells are induced by allergens but not during parasite infection. Transgenic mice lacking these cells show impaired production of high-affinity, anaphylactic IgE. T _FH 13 cells, which are elevated in patients with food and aeroallergies, may be targeted in future antianaphylaxis therapies. Science , this issue p. eaaw6433

PURPOSE: With improvements in neonatal intensive care, more premature infants are surviving. Many have significant pulmonary morbidities after discharge from the hospital. Bronchopulmonary dysplasia is the most common morbidity of prematurity and is a common indication for home oxygen therapy in children. Data are lacking on the appropriate methods for weaning supplemental oxygen. The objective of this study was to identify the methods used by pediatric pulmonologists to wean premature infants from supplemental oxygen. SUBJECTS: One hundred eighty-four questionnaires were sent to pediatric pulmonologists at 20 pediatric pulmonary programs. DESIGN AND METHODS: This cross-sectional, self-administered anonymous survey assessed strategies for oxygen weaning for premature infants. MAIN OUTCOME MEASURES AND PRINCIPLERESULTS: We had a 70% (14/20) institutional response rate. Of the responding institutions, we had a 73% (99/136) response rate. The majority of pulmonologists surveyed (78%) reported using nocturnal oxygen saturations as the primary indication for weaning. Only 8% of pulmonologists reported using a standardized protocol. Half of the respondents reported weaning diuretics prior to oxygen. The factors considered prior to initiating oxygen weaning included growth (96%), vital signs (85%), hospitalizations (68%), and echocardiograms (59%). Fewer respondents (21%) reported using chest x-ray findings. The minimum oxygen saturation in room air required to take a patient off oxygen ranged from 90% to 95%, with a mean of 93% (SD = 1.74). CONCLUSION: Pediatric pulmonologists reported a wide range of practice patterns in weaning premature infants from supplemental home oxygen. Very few respondents reported using a standardized protocol. There is no consensus about whether to wean diuretics or oxygen first. The majority of providers use nocturnal oxygen saturations as an indication for readiness to wean. Growth is an important factor for oxygen weaning considered by most pulmonologists surveyed. Consensus guidelines and subsequent evaluation of such guidelines are needed to ensure the safety of this growing population of infants.

Ultrastructural examination of cilia is the “gold standard” for diagnosing primary ciliary dyskinesia. There is little evidence suggesting the most effective method of procuring a ciliary biopsy and scant benchmark data on rates of conclusive biopsies or on the diagnostic impact of such biopsies. To critically assess rates of inconclusive, positive, and negative ciliary biopsies and to identify clinical factors associated with conclusive results, we reviewed ciliary biopsies submitted for electron microscopy from 2006 to 2011, noting whether specimens were adequate for analysis and whether the ciliary structure was normal. The biopsy site, method used, procedurist's specialty, and clinical diagnoses were determined. Biopsy findings were categorized by diagnostic impact. Over 5 years, 187 patients had 211 biopsies. Conclusive results were obtained on 133/211 biopsies (63%); the remainder were insufficient. The rate of inconclusive biopsies did not vary significantly ( P > 0.05; Fisher's exact) among sampling methods. Abnormal results were identified in 8/133 (6.0%) of the adequate specimens. Forceps compared to brush biopsies (abnormal in 4/12 versus 4/121 of the adequate specimens, P = 0.002), along with multiple biopsy samples (taken on same or different days) compared with a single biopsy sample (abnormal in 3/12 versus 1/110 of the adequate specimens, P = 0.01), were more likely to yield an abnormal result. Only 63% of pediatric ciliary biopsies provide adequate morphology for analysis, the large majority of these samples showing normal ciliary anatomy. The method of obtaining biopsies did not significantly affect result conclusiveness. Understanding the diagnostic impact of ultrastructural analysis is important as new diagnostic algorithms are developed for primary ciliary dyskinesia.

Objective: Pediatric providers play an important role in parental and youth smoking cessation. The goal of this study was to understand smoking cessation attitudes of parents and the behaviors, confidence and self-efficacy of pediatricians related to providing smoking cessation counseling to parents and youth. Methods: A mixed methods study was conducted in a convenience sample of families (n = 1,549) and pediatric primary care clinicians (n = 95) in Connecticut using surveys and focus groups from April, 2016 to January, 2017. Results: The smoking rate (cigarettes or electronic cigarettes) among all households surveyed was 21%. Interest in quitting smoking was high (71%) and did not differ based on smoking amount, duration, type of community of residence (urban, rural, etc), or race/ethnicity. For example, compared to participants who smoked for <10 years, those who smoked ≥20 years had a similar interest in quitting (OR = 1.12; 95% CI: 0.85–1.48). Ninety percent of clinicians surveyed asked parents about their smoking behavior at least annually but 36% offered no smoking cessation counseling services or referral. Clinicians almost always reported counseling youth about the dangers of nicotine and tobacco use (99%), were more confident about counseling youth than parents (p<0.01) and reported low self-efficacy about smoking cessation and prevention counseling of parents and youth. Ninety-three percent of clinicians opined that electronic cigarettes were equally or more dangerous than cigarettes but 34% never counseled youth about the dangers of electronic cigarettes. Conclusions: Clinicians frequently screen parents about their smoking behaviors, but rarely provide smoking cessation counseling and express low confidence in this activity. Clinicians are more confident counseling youth than parents. Clinicians also recognize the dangers of electronic cigarettes, yet they infrequently counsel youth about these dangers.