Precision Agriculture (PA) is a crop site-specific management system that aims for sustainability, adopting agricultural practices more friendly to the environment, like the variable rate application (VRA) technique. Many studies have dealt with the effectiveness of VRA to reduce nitrogen (N) fertilizer, while achieving increased profit and productivity. However, only limited attention was given to VRA’s environmental impact. In this study an International Organization for Standardization (ISO) based Life Cycle Assessment (LCA) performed to identify the environmental effects of N VRA on a small pear orchard, compared to the conventional uniform application. A Cradle to Gate system with a functional unit (FU) of 1 kg of pears was analyzed including high quality primary data of two productive years, including also the non-productive years, as well as all the emissions during pear growing and the supply chains of all inputs, projecting them to the lifespan of the orchard. A methodology was adopted, modelling individual years and averaging over the orchard’s lifetime. Results showed that Climate change, Water scarcity, Fossil fuels and Particulate formation were the most contributing impact categories to the overall environmental impact of the pear orchard lifespan, where climate change and particulates were largely determined by CO₂, N₂O, and NH₃ emissions to the air from fertilizer production and application, and as CO₂ from tractor use. Concerning fertilization practice, when VRA was combined with a high yield year, this resulted in significantly reduced environmental impact. LCA evaluating an alternative fertilizer management system in a Greek pear orchard revealed the environmental impact reduction potential of that system.

Pre-harvest aflatoxin contamination (PAC) is a major problem facing peanut production worldwide. Produced by the ubiquitous soil fungus, Aspergillus flavus, aflatoxin is the most naturally occurring known carcinogen. The interaction between fungus and host resulting in PAC is complex, and breeding for PAC resistance has been slow. It has been shown that aflatoxin production can be induced by applying drought stress as peanut seeds mature. We have implemented an automated rainout shelter that controls temperature and moisture in the root and peg zone to induce aflatoxin production. Using polymerase chain reaction (PCR) and high performance liquid chromatography (HPLC), seeds meeting the following conditions were selected: infected with Aspergillus flavus and contaminated with aflatoxin; and not contaminated with aflatoxin. RNA sequencing analysis revealed groups of genes that describe the transcriptional state of contaminated vs. uncontaminated seed. These data suggest that fatty acid biosynthesis and abscisic acid (ABA) signaling are altered in contaminated seeds and point to a potential susceptibility factor, ABR1, as a repressor of ABA signaling that may play a role in permitting PAC.

The objectives of this study were to compare variable rate and uniform fertilizer applications in apple (Malus domestica Borkh.) and its effect on production and economic return. In the study, 59.6% and 63.4% less fertilizer were applied in the variable rate treatments (VRT) in 2011 and 2012, respectively, with less than 4% difference in yield. Soil properties did not significantly affect yield and fruit quality. The VRT fertilizer cost was reduced by 2.3% and 7.6% in 2011 and 2012, respectively, and fruit quality was not significantly affected. Assessing yield to calculate fertilizer demand suggests variable rate fertilizer inputs can potentially increase the profitability of an apple orchard.

Remotely sensed-based surface temperature is an important tool for crop monitoring and has great potential for improving irrigation management. However, current thermal satellite platforms do not display the fine spatial resolution required for identifying crop water status patterns at the field scale. The thermal sharpening (TsHARP) utility provides a technique for downscaling coarse thermal images to match the finer resolution of images acquired in the visible and near infrared bandwidths. This sharpening method is based on the inverse linear relationship between vegetation fraction calculated from the normalized difference vegetation index (NDVI) and land surface temperature (LST). The current study used the TsHARP method to sharpen low-resolution thermal data from the Moderate Resolution Imaging Spectrometer MODIS (1 km) to the finer resolution of Sentinel-2 (10 m) and Vegetation and Environment New micro-Spacecraft (VENµS) (5 m) visible-near infrared images. The sharpening methodology was evaluated at scene and field scales in southern Georgia and northern Mississippi, USA. A comparison of sharpened temperature was made with reference temperatures from Landsat-8 Operational Land Imager (OLI) in four different spatial resolutions (30, 60, 120, and 240 m) for method validation. Coarse resolution comparison on the dates in which imagery from both sensors were acquired on the same day resulted in average observed mean absolute error (MAE) of 1.63 °C, and R² variation from 0.34 to 0.74. Temperature errors at the field scale ranged from 0.25 to 3.11 °C using both Sentinel-2 and VENµS. Sharpened maps at 120 and 60 m resolution showed the highest consistency for all fields and dates. Maps sharpened using VENµS images showed comparable or higher accuracy than maps sharpened using Sentinel-2. The superior performance coupled with the better revisit time indicates that the VENµS platform has high potential for frequent in-season crop monitoring. Further research with ground data collection is needed to explore field use limitations of this methodology, but these results give useful insights of potential benefits of implementing the TsHARP technique as a tool for crop stress monitoring.