This study aimed to characterize and investigate the potential of the oils from Gryllus bimaculatus, Teleogryllus mitratus, and Acheta domesticus to be used in nanoemulsions. The oils were extracted by a cold press method and characterized for their fatty acid profiles. Their irritation effects on the chorioallantoic membrane (CAM) were evaluated, along with investigations of solubility and the required hydrophilic–lipophilic balance (RHLB). Various parameters impacting nanoemulsion generation using high-pressure homogenization were investigated. The findings revealed that G. bimaculatus yielded the highest oil content (24.58% w/w), followed by T. mitratus (20.96% w/w) and A. domesticus (15.46% w/w). Their major fatty acids were palmitic, oleic, and linoleic acids. All oils showed no irritation, suggesting safety for topical use. The RHLB values of each oil were around six–seven. However, they could be successfully developed into nanoemulsions using various surfactants. All cricket oils could be used for the nanoemulsion preparation, but T. mitratus yielded the smallest internal droplet size with acceptable PDI and zeta potential. Nanoemulsion was found to significantly enhance the antioxidant and anti-skin wrinkle of the T. mitratus oil. These findings pointed to the possible use of cricket oils in nanoemulsions, which could be used in various applications, including topical and cosmetic formulations.

This paper aims to conduct a gap analysis and explore the potential for greenhouse gas (GHG) emissions reduction in the tourism sector of Chiang Mai province, with the goal of promoting sustainable tourism. Chiang Mai is a major tourism hub in Thailand, located in the Northern Economic Corridor (NEC). The gap analysis of small- and medium-sized tourism enterprises will be examined across four dimensions: (1) management, (2) socio-economy, (3) cultural, and (4) environmental. In 2024, Chiang Mai’s tourism revenue accounted for 46.97% of the northern region’s total tourism revenue and 3.73% of Thailand’s total tourism revenue. Given this economic significance, the development of sustainable tourism should be accelerated to meet the expectations of new tourists who are increasingly concerned about the environment. To address this need, this study analyzes the gaps in small- and medium-sized tourism enterprises and assesses GHG emissions through interviews and surveys of 90 tourism-related establishments across nine sectors: hotels, restaurants and beverages, tour agencies, transportation, souvenirs, attractions and activities, spas and wellness, community-based tourism, and farm tourism. The total GHG emissions from these establishments were found to be 15,303.72 tCO₂eq. Moreover, if renewable energy from solar power were adopted, an installation capacity of 21,866.84 kWp would be required. Such a transition would not only reduce emissions, but also support low-carbon development in small- and medium-sized tourism enterprises and ultimately contribute to achieving net-zero tourism. Finally, this study contributes to the advancement of STGs 1–17, adapted from the SDGs 1–17, with particular emphasis on SDG 7 on clean energy and SDG 13 on climate change.

Perilla frutescens, “Nga-Kee-Mon” in Thai, is a high-nutritional-value plant. This study aims to identify the phytochemicals, apoptosis induction and immunomodulating activities of the perilla seed extract (PSE) and highlight the high pharmaceutical value of perilla. The phytochemical profile of PSE was characterized using HPLC. Antioxidant capacity was studied using DPPH assay. Apoptosis was confirmed by morphological changes and DNA fragmentation of the human colon adenocarcinoma cell line (HT-29). Immunomodulating activity was studied in an LPS-stimulated murine macrophage cell line (RAW 264.7). PSE had high levels of TPC (375.04 ± 11.45 mg GAE/g) and TFC (223.45 ± 16.02 mg QE/g) with strong radical scavenging capacity (312.87 ± 12.98 mg TE/100 g). Rosmarinic acid (0.116 g%) and luteolin (0.010 g%) were the major phytochemicals. PSE at 50 µg/mL, equivalent to 0.85 and 0.08 µg/mL of rosmarinic acid and luteolin, respectively, caused morphological alterations and DNA fragmentation within 24 h. PSE at 200 µg/mL, equivalent to 3.38 and 0.30 µg/mL of rosmarinic acid and luteolin, respectively, had significant inhibitory activity on IL-1β, IL-6, and TNF-α secretion. These results demonstrate that PSE has high antioxidant capacity, with rosmarinic acid and luteolin as the major phytochemicals. It can trigger apoptosis in HT-29 cells and has immunomodulatory effects. These findings highlight the potential of perilla seed extract as a promising natural source for therapeutic applications related to oxidative stress, cancer prevention, and immune modulation.

Psophocarpus tetragonolobus (L.) DC or winged bean is a nutrient-rich tropical legume that has been a part of the Southeast Asian cuisine for centuries. In this study, phytochemical composition and anticancer and anti-inflammatory properties of winged bean pod ethanolic extract (WBE) were investigated with specific emphasis on colorectal cancer. Phytochemical fingerprint of WBE was evaluated by high-performance liquid chromatography (HPLC). Cytotoxicity and induction of apoptosis were evaluated in human colorectal adenocarcinoma (HT-29) by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, morphological observation, and deoxyribonucleic acid (DNA) fragmentation. Anti-inflammatory activity was measured in lipopolysaccharide-stimulated mouse mononuclear macrophage (RAW 264.7) by determination of pro-inflammatory cytokines. WBE contained high total phenolic (237.33 ± 8.48 mg gallic acid/g) and flavonoid (180.53 ± 6.31 mg quercatin/g) content, with the primary flavonoid being kaempferol (0.012 g%). WBE dose-dependently suppressed viability (IC₅₀ = 117.86 µg/mL) and triggered apoptosis in HT-29 cells, with morphological alterations and DNA fragmentation. In addition, WBE significantly suppressed secretion of interleukin-1β, interleukin-6, and tumor necrosis factor-α. Therefore, WBE displayed therapeutic potential for the induction of apoptosis against colorectal cancer cells and inhibitions of inflammatory cytokines. Despite being traditionally consumed as an edible plant, additional studies are needed to confirm the safety of P. tetragonolobus extract.