Cystic fibrosis (CF) is a chronic and potentially life-threatening condition, wherein timely diagnosis assumes paramount significance for the prompt initiation of therapeutic interventions, thereby ameliorating pulmonary function, addressing nutritional deficits, averting complications, mitigating morbidity, and ultimately enhancing the quality of life and extending longevity. This review aims to amalgamate existing knowledge to provide a comprehensive appraisal of contemporary diagnostic modalities pertinent to CF in the 21st century. Deliberations encompass discrete delineations of each diagnostic modality and the elucidation of potential diagnostic quandaries encountered in select instances, as well as the delineation of genotype–phenotype correlations germane to genetic counseling endeavors. The synthesis underscores that, notwithstanding the availability and strides in diagnostic methodologies, including genetic assays, the sweat test (ST) retains its position as the preeminent diagnostic standard for CF, serving as a robust surrogate for CFTR functionality. Prospective clinical investigations in the realm of CF should be orchestrated with the objective of discerning novel diagnostic modalities endowed with heightened specificity and sensitivity.

The purpose of this paper was to present the results of the evaluation of 1320 adolescents from 4 high schools in Iaşi, Romania regarding their eating habits and perceived benefits of physical activity and body attractiveness after they completed an anonymous questionnaire. The data analysis was performed using IBM SPSS version 18. The analysis of the nutritional status showed that over 70% of adolescents were of a normal weight. Most of them (63.56%) consumed 3–4 meals/day, with breakfast being the most skipped meal (60.53%). Compared with the boys, the girls consumed fewer whole grains, dairy products, and meat, but the girls consumed fruits and vegetables in approximately the same percentage. The adolescents studied consumed more fruits than vegetables, but the servings of fruits and vegetables consumed were not sufficient in the diets of many adolescents (39.31%). Among the adolescents, 5.68% consumed at least 2 alcoholic drinks daily, and 37.87% consumed caffeine products one or more times per week. Among the boys, 95.20% practiced regular physical activity, and 97.20% of the girls considered that the right nutrition plays a role in the prevention of obesity. The study of the self-perception profile showed that 89.8% of boys and 79.5% of girls considered that they had attractive bodies. According to the BMI, 86.7% of normal-weight adolescents and 84.8% of underweight adolescents considered themselves as having attractive bodies, while this was only considered by 73.5% of overweight and 56.7% of obese adolescents. It is necessary to introduce nutritional education in schools for the improvement of eating habits in children and adolescents. More studies are needed to appreciate the social behaviors (e.g., food intake, physical activity, and recreation time) and then develop interventions and national health programs to promote a healthy lifestyle, particularly healthy eating habits.

Candida auris is an emerging multidrug-resistant fungal pathogen with a high affinity for skin colonization and significant potential for nosocomial transmission. This study aimed to develop and evaluate chitosan-based hydrogels loaded with nystatin and propolis as a topical antifungal strategy for skin decolonization of C. auris. The formulations were selected based on our previous results and optimized for cutaneous application. The internal structure of the hydrogels was investigated by polarized light microscopy, confirming the amorphous nature of propolis and the partial dispersion of nystatin. The antifungal activity was assessed against ten fluconazole-resistant C. auris strains. The CS-NYS-PRO1 formulation demonstrated the highest antifungal performance in the agar test, also reducing viable cell counts to undetectable levels within 6 h. Time–kill assays and SEM imaging confirmed the rapid fungicidal effect and revealed severe membrane disruption and cytoplasmic leakage. Molecular docking analyses indicated the strong binding of nystatin to both sterol 14α-demethylase (CYP51) and dihydrofolate reductase (DHFR) from C. auris, suggesting complementary membrane and intracellular mechanisms of action. These findings support the use of such hydrogels as a local, non-invasive, and biocompatible strategy for managing C. auris colonization, with promising implications for clinical use in infection control and the prevention of skin-mediated transmission in healthcare settings.