Endophytic microorganisms inhabiting plant tissues constitute a unique and largely untapped reservoir of bioactive metabolites, including phenolics, terpenoids, alkaloids, polysaccharides, and anthraquinones, among others. This review focuses on the potential of these compounds to modulate the complex processes of wound repair, such as hemostasis, inflammation, proliferation, and remodeling. Uniquely, this review delineates the specific mechanisms supported not only by indirect evidence but by primary research directly linking endophytic metabolites to wound repair. We synthesized and evaluated evidence from 18 studies, of which over 75% directly assessed wound healing effects through in vitro and in vivo models. Metabolites from endophytic microorganisms promoted wound contraction, suppressed biofilm formation by key pathogens (e.g., MRSA, P. aeruginosa), and accelerated tissue re-epithelialization in animal models. Other compounds demonstrated >99% wound closure in rats, while several extracts showed anti-inflammatory and cytocompatible profiles. Nevertheless, the majority of studies applied unstandardized methods and used crude extracts, hindering precise structure–activity assessment. The originality of this review lies in drawing attention to direct evidence for wound healing from diverse endophytic sources and systematically identifying gaps between preclinical promise and clinical translation, positioning endophytes as a sustainable platform for next-generation wound therapeutics.

Flaxseed consumption has been prioritized by health-conscious people all over the world. Its consumption raises interest to it both from a personal and an industrial prospect due to its remarkable fatty acids profile. Besides its high content of unsaturated fatty acids, flaxseed oil is known for its low omega 6/omega 3 ratio, which may possess health improving properties because this oil is precursor of anti-inflammatory molecules. However, the high amount of polyunsaturated fatty acids in flaxseed oil causes its high sensitivity to high temperatures, to light and to oxygen, which may lead to its oxidation and quality degradation. Screw pressing can produce high quality oil but in lesser volume, which could be disadvantage. Therefore, the extraction process must be optimized for maximizing oil recovery, while maintaining oxidation indicators within limits specified by regulations, as well as a potentially health beneficial fatty acid profile. Optimization of screw press parameters for pressing out the flaxseed oil were investigated by Response Surface Methodology. Both size of the cake outlet and the speed of the screw pressing were optimized, and the values that provided the highest experimental oil recovery, 41.4%, were of 1 mm and 155.89 rpm respectively. Although the overall trend in oil extraction showed a rising oil recovery when screw speeds increased from 98.73 up to 213.05 rpm, certain fluctuations were observed in oil extraction with varying outlet cake sizes. However, there was an inversely-proportional function between the oil recovery and the size of the cake outlet, therefore, screw speed provided a minor, non-significant effect while the size of the cake outlet proved to have a strong effect on oil recovery. The highest acid value and peroxide value accounted to 0.71 mg KOH/g and to 7.71 meq/kg respectively. Sediment content (SC) of screw pressed flax oil ranged between 9.12~14%. During the oil extraction at the maximum yield, temperature increased, however the ratio of omega 6 to omega 3 in the obtained oil still remained low.

Considering the high global consumption of mayonnaise and modern concerns about the health value of food products, the recipe for mayonnaise sauces is constantly evolving. In this study, we present the proximal analysis and toxicological evaluation of a new mayonnaise, which was patented. Starch was replaced with sesame meal, and lemon zest was added as an antioxidant. Compared to a mayonnaise prepared using a traditional recipe as control, the enriched mayonnaise had higher protein and lower fat content, suggesting a potentially healthier product. Aditionally, peroxide and anisidine values were lower in the new mayonnaise, indicating higher resistance to oxidative damage. Toxicological studies of the enriched mayonnaise, including oral moderate-to-lethal dose (acute oral toxicity), cumulative (subacute) action, irritating effect on mucous membranes, and sensitizing properties, which were conducted on laboratory animals following required regulatory standards, are shown in this work. The patented sesame meal and lemon zest-enriched mayonnaise proved to be safe in all performed studies, meeting safety requirements regarding toxicological parameters. Furthermore, the enriched mayonnaise is affordable, with a production cost similar to that of control mayonnaise.

Nanofiber scaffolds play a crucial role in bioengineering by providing structural support for tissue and organoid growth. For composite nanofibers, optimizing their properties for specific applications often requires analyzing the spatial distribution of their chemical structure. This review focuses on the applications of Raman hyperspectral imaging to the mapping of the chemical composition of nanofibers. While the technique is diffraction-limited to the size of the scanning beam, it is possible to decipher the nanoscale features of these fibers by employing oversampling during scanning. Subsequently, these oversampled data can be analyzed by a singular-value decomposition (SVD) analysis and classical least-squares (CLS) decomposition. In many cases, this technique is essential for verifying the spatial distribution of different chemical components within multi-component nanofibers.