Iron (oxyhydr)oxide microbial mats in modern to ∼100 ka tufa terraces are present in a cold spring system along Ten Mile Graben, southeastern Utah, USA. Mats exhibit morphological, chemical, and textural biosignatures and show diagenetic changes that occur over millennial scales. The Jurassic Brushy Basin Member of the Morrison Formation in the Four Corners region of the USA also exhibits comparable microbial fossils and iron (oxyhydr)oxide biosignatures in the lacustrine unit. Both the modern spring system and Brushy Basin Member represent alkaline, saline, groundwater-fed systems and preserve diatoms and other similar algal forms with cellular elaboration. Two distinct suites of elements (1. C, Fe, As and 2. C, S, Se, P) are associated with microbial fossils in modern and ancient iron (oxyhydr)oxides and may be potential markers for biosignatures. The presence of ferrihydrite in ∼100 ka fossil microbial mats and Jurassic rocks suggests that this thermodynamically unstable mineral may also be a potential biomarker. One of the most extensive sedimentary records on Mars is exposed in Gale Crater and consists of non-acidic clays and sulfates possibly of lacustrine origin. These terrestrial iron (oxyhydr)oxide examples are a valuable analogue because of similar iron- and clay-rich host rock compositions and will help (1) understand diagenetic processes in a non-acidic, saline lacustrine environment such as the sedimentary rocks in Gale Crater, (2) document specific biomediated textures, (3) demonstrate how biomediated textures might persist or respond to diagenesis over time, and (4) provide a ground truth library of textures to explore and compare in extraterrestrial iron (oxyhydr)oxides, where future explorations hope to detect past evidence of life.

On Earth, microorganisms commonly enhance mineral precipitation and mediate mineralogical and chemical compositions of resulting deposits, particularly at spring systems. However, preservation of any type of microbial fossil or chemical or textural biosignature depends on the degree of alteration during diagenesis and factors such as exposure to diagenetic fluids. Little is known about the transformation of biosignatures during diagenesis over geologic time. Ten Mile Graben, Utah, USA, hosts a cold spring system that is an exceptional site for evaluation of diagenetic alteration of biosignatures because of the presence of modern springs with actively precipitating microbial mats and a series of progressively older tufa terraces (<400 ka) preserved in the area from the same spring system. A previously undescribed Jurassic laminated carbonate unit within the upper part of the Brushy Basin Member of the Morrison Formation is also exposed in Ten Mile Graben. This research characterizes the geology of these modern and Quaternary saline, Fe-undersaturated, circumneutral Ten Mile Graben cold springs and provides the first description in the literature of the Jurassic Brushy Basin Member of the Morrison Formation carbonate deposit. Taphonomy of microbial fossils is characterized by scanning electron microscopy (SEM). The data highlight two distinct methods of biosignature formation: (1) precipitation of minerals from an undersaturated solution owing to metabolic activity of the cells and (2) mineral precipitation on charged cell surfaces that produce distinctive microbial trace fossils. Although diagenesis can destroy or severely degrade biosignatures, particularly microbial fossils, some fossils and trace fossils are preserved because entombment by Ostwald ripening limits diagenetic alteration. Recognizing spring-fed, biogenic tufas is crucial for astrobiological research and the search for life on Mars. Key Words: Biosignatures—Taphonomy—Diagenesis—Carbonates—Hot springs. Astrobiology 17, 216–230.

Members of the alunite group are precipitated at low pH (<1 to ~4) in oxidizing environments, are unstable in circumneutral conditions, and are widespread on Mars. At Mollies Nipple in Kane County, Utah, USA, jarosite and alunite are abundant as diagenetic cements in Jurassic sandstones. This research characterizes the jarosite and alunite cements with the goal of determining their origin, and tests the hypothesis that jarosite and alunite may be more stable than the current understanding indicates is possible. Previous studies have placed the jarosite- and alunite-bearing caprock at Mollies Nipple in the Navajo Sandstone, but the presence of water-lain deposits, volcanic ash, volcanic clasts, and peloids show that it is one of the overlying Middle Jurassic units that records sea level transgressions and regressions. A paragenetic timing, established from petrographic methods, shows that much of the cement was precipitated early in a marginal marine to coastal dune depositional environment with a fluctuating groundwater table that drove ferrolysis and evolved the groundwater to a low pH. Microbial interaction was likely a large contributor to the evolution of this acidity. Jarosite and alunite are clearly more stable in natural environments than is predicted by laboratory experiments, and therefore, the Martian environments that have been interpreted as largely acidic and/or dry over geologic time may have been more habitable than previously thought.

Clay mineralogy and diagenesis affect the reservoir quality of the Neogene Surma Group in the Hatiya trough of Bengal Basin, Bangladesh. X-ray diffraction and scanning electron microscopic analyses of diagenetic clay minerals from Shahbazpur#2 well reveal that on average illite is the dominant clay mineral (50%), followed by chlorite (24%), kaolinite (23%) and smectite (2.50%). The absence of smectite at Core-2 (3259.80 m to 3269 m) results from the total transformation of smectite to illite owing to burial depth and high K–feldspar. The diagenetic changes are a result of chemical processes such as cementation, chlorite authigenesis, dissolution, alteration and replacement that have significantly affected the reservoir properties. Cementation plays an important role in reducing reservoir properties with pore and fracture filling cement. The relative percentage of illite and smectite minerals (>90% illite in I/S mixed layer) and Kübler index value (0.34° to 0.76° Δ2θ) indicate a diagenetic zone with subsurface temperatures of 120–180 °C in the studied samples. The temperature range determined using clay percentages and the Kübler index as a geothermometer is supported by observed diagenetic features such as quartz overgrowths, smectite to illite transformations and chlorite coatings. The diagenetic features cause variable reservoir porosity and permeability that are critical in planning exploration and development programs of this field or analog fields across the Bengal Basin.

Stable δ ¹³ C isotope analysis at hot and cold springs suggests that rapid degassing overprints carbon isotopic biosignatures even when microbial activity produces biogenic textures in the minerals. Mineral precipitation and potential biosignature preservation are evaluated at a cold spring system in Ten Mile Graben, Utah, USA, with scanning electron microscopy, X-ray diffraction, and stable carbon isotopes. Putative biogenic mineral habits such as aragonite microspheres and botryoids, and biologic materials (EPS and diatom tests) are abundant in modern mats, but the δ ¹³ C values are between +2‰ and +7.8‰, consistent with rapid CO ₂ degassing reported by other researchers. Multiple factors, however, influence isotopic signatures of mineral precipitates in this spring system, including rapid degassing, preferential microbial uptake of light carbon isotopes via multiple carboxylation pathways, hydrocarbon-charged fluid, and other inherited isotopic signatures in the fluid, particularly from dissolution of older limestones; therefore, it is not likely that this narrow range of isotopic ratios definitively shows an abiotic signature. A fossil vent preserves biogenic mineral habits, but not microbial body fossils. This study highlights the need for novel biosignature detection methods—and an understanding of what an abiotic signature definitively is—as we prepare for sample caching of carbonate rocks by the Mars2020 mission and future sample return.

Dense urban areas like Berlin face unique challenges when instituting geothermal energy, especially understanding how subsystems (e.g., transportation, people, legal framework, deep subsurface, drinking water, etc.) are linked and how the larger system is affected by changes to subsystems. The innovative approach to viewing a city as a system composed of subsystems and integrating the subsystems in a holistic energy efficiency framework is required to prevent unforeseen adverse consequences of actions such as heat pollution from thermal energy storage in deep reservoirs. The interaction between U-Bahn tubes, HT-ATES/BTES systems, groundwater flow, and the urban heat island creates a tightly coupled thermomechanical environment in which temperature, pore pressure, strain, and geochemical states evolve simultaneously and govern system efficiency and structural stability. In Berlin-Adlershof, an HT-ATES research well was completed in 2024 and drilling of the injection and production wells are slated for this year. Our new work will demonstrate the efficacy of: 1. HT-ATES in Berlin for thermal heat storage and production, 2. Using the subway system to collect heat and mitigate heat waste from the shallow subsurface heat island and heat pollution produced by HT-ATES wells. The interaction data from monitoring wells and a fiber optic experiment within the subway will provide parameters for thermomechanical models on granular scales (e.g., directly adjacent to wells, subway) and we will scale up models to interconnect ATES, subway, and groundwater, and then to district, city, and national levels. Social science and humanities data will be incorporated into the large-scale models to produce a structure for establishing UTES in other cities with unique settings in terms of geology, legal framework, drinking water sources, presence/ absence of mining, and other differences.