To extend the shelf life of button mushrooms, the optimal fumigation doses for hydrogen gas treatment were screened through sensory evaluation, combined with browning index and weight loss rate in this study. Then, using H₂ fumigation combined with polyethylene film packaging as a control, changes in the sensory quality, reactive oxygen species, browning-related enzyme activity and the nutritional quality of mushrooms treated by H₂ fumigation combined with nano-film packaging (H₂ + NA) during low-temperature storage were dynamically tracked. The preservation effect of H₂ + NA on mushrooms after harvest was investigated, and its mechanism was also analyzed. The storage validation test showed that the optimum H₂ fumigation time was 2 h, and the H₂ + NA-treated mushrooms had a fuller appearance, maintained whiteness well, showed a slow increase in reactive oxygen species, antioxidant enzyme activities remained at high levels, a high retention rate of protein content was observed, and there was a good antibacterial effect. This study indicates that H₂ fumigation combined with nano-film packaging can improve the storage quality of button mushrooms and may prolong low-temperature shelf life by 4–5 d compared to conventional commercial polyethylene film packaging.

In order to innovatively develop high-activity ACE inhibitory peptides from edible fungi, the conditions for a double-enzymatic hydrolysis preparation of ACE inhibitory peptides from Flammulina velutipes were optimized by response surface methodology. After purification by macroporous resin, gel chromatography, and RP-HPLC, a crude peptide fraction was obtained; its ACE inhibition rate was 85.73 ± 0.95% (IC₅₀ = 0.83 ± 0.09 mg/mL). Based on LC-MS/MS sequencing, the four novel peptides, namely, FAGGP, FDGY, FHPGY, and WADP, were screened by computer analysis and molecular docking technology. The four peptides exhibited a binding energy between −9.4 and −10.3 kcal/mol, and formed hydrogen bonds with Tyr523, Ala354, and Glu384 in the S1 pocket, Tyr520 and His353 in the S2 pocket, and His383 in the HEXXH zinc-coordinating motif of ACE, indicating their good affinity with the ACE active site. The IC₅₀ values of the four ACE inhibitory peptides were 29.17, 91.55, 14.79, and 41.27 μM, respectively, suggesting that these peptides could potentially contribute to the development of new antihypertensive products.