Understanding the relationship between work stress and job satisfaction is crucial for promoting employee well-being and also for sustainable organizational performance. This study proposes and validates, within the population of employees in Serbia, the Stress Symptoms Survey (SSS), an 18-item instrument for measuring physical and psychological symptoms of work-related stress. The scale shows strong internal consistency where a general factor is highly saturated with all survey items. Regression analysis indicated that lack of organizational support was the strongest predictor of lowered job satisfaction, followed by stress symptoms and general job stress; job pressure showed a positive association with job satisfaction when other stressors were controlled. These results highlight the practical value of the SSS and underscore the crucial role of supportive work environments in mitigating stress, enhancing satisfaction and achieving sustainable work performance.