Background: Xyleborus affinisEichhoff (Coleoptera: Curculionidae) is an ambrosia beetle reported to affect avocado trees (Persea americanaMill.). The use of the entomopathogenic fungus (EPF)Beauveria bassiana(Bals.-Criv.) Vuill. for ambrosia beetle control represents an alternative to insecticides.Methods: This study was designed in two stages to selectB. bassianastrains with potential to controlX. affinisfemales. In the first stage, 19B. bassianaMexican strains from EPF collection, isolated from Coleoptera (CHE-CNRCB,http://www.gob.mx/senasica/documentos/coleccion-de-hongos-entomopatogenos), were tested. Analyses included radial growth rate, conidial yield, spore germination, and germ tube length. Results were analysed by Principal Component Analysis (PCA) to identify clusters within favourable growth phenotypes. For the second stage, 10 selected strains were re-analysed for virulence-related metabolic characteristic, including cell wall-bound cuticle-degrading enzymes–Pr1-like proteases and β-N-acetyl glucosaminidases (NAGase) chitinases, conidial hydrophobicity and monopolar germination parameters. A second PCA analysis was run for those virulence parameters analysed, and upon results strains CHE-CNRCB 44, 171, 431 and 485 were selected and tested againstX. affinisfemales. Females were treated with a 1 × 108conidia mL−1suspension (recommended rate), using a Potter Tower.Results: All strains showed insecticidal activity, inducing up to 58% mortality; about 30% dead beetles developed aerial mycelia (CHE-CNRCB 485) and the fastest mortality rate wast0= 1.95 (CHE-CNRCB 44).Conclusion: Since all selected strains showed virulence againstX. affinisfemales, results indicated the possibility of selectingB. bassianastrains based on multiple metabolic attributes, as a preliminary test to perform bioassays against order-related target insects.
1 January 2019 in International Journal of Food Properties
A sensory evaluation was performed for coffee samples using the Check-All-That-Apply (CATA) method. The samples included Mexican coffee from Hidalgo and Colima states. The brews were prepared by the espresso and French press methods. The data obtained were analyzed using Cochran´s Q test in order to determine any significant differences between samples. There were significant differences (P < .05) only in visual attributes, due to intrinsic characteristics of the preparation methods of the samples. Additionally, the results were analyzed using Multiple Correspondence Analysis (MCA), where from a two-dimensional graphic, differences and similarities between samples and attributes were observed. In the same way, it was observed that visual attributes had greater influence for the description of the samples.