Cannabis sativa L. is a versatile plant with significant medicinal, industrial, and recreational applications. Its therapeutic potential is attributed to cannabinoids like THC and CBD, whose production is influenced by environmental factors, such as radiation, temperature, and humidity. Radiation, for instance, is essential for photosynthetic processes, acting as both a primary energy source and a regulator of plant growth and development. This review covers key factors affecting C. sativa cultivation, including photoperiod, light spectrum, cultivation methods, environmental controls, and plant growth regulators. It highlights how these elements influence flowering, biomass, and cannabinoid production across different growing systems, offering insights for optimizing both medicinal and industrial cannabis cultivation. Studies indicate that photoperiod sensitivity varies among cultivars, with some achieving optimal flowering and cannabinoid production under extended light periods rather than the traditional 12/12 h cycle. Light spectrum adjustments, especially red, far-red, and blue wavelengths, significantly impact photosynthesis, plant morphology, and secondary metabolite accumulation. Advances in LED technology allow precise spectral control, enhancing energy efficiency and cannabinoid profiles compared to conventional lighting. The photoperiod plays a vital role in the cultivation of C. sativa spp., directly impacting the plant’s developmental cycle, biomass production, and the concentration of cannabinoids and terpenes. The response to photoperiod varies among different cannabis cultivars, as demonstrated in studies comparing cultivars of diverse genetic origins. On the other hand, indoor or in vitro cultivation may serve as an excellent alternative for plant breeding programs in C. sativa, given the substantial inter-cultivar variability that hinders the fixation of desirable traits.

Industrial hemp (Cannabis sativa L.) is a multipurpose crop with growing interest due to its environmental adaptability, low input requirements, and potential contribution to sustainable agricultural systems. This study evaluated the agronomic performance of four industrial hemp varieties grown under the edaphoclimatic conditions of the Alentejo region over two consecutive growing seasons (2024 and 2025) using different sowing dates. Phenological stages, plant height and growth parameters were monitored, complemented by meteorological data obtained from IPMA. The results revealed clear differences between years. The later sowing date in 2024 promoted greater vegetative growth, resulting in taller plants, while the earlier sowing in 2025 extended the vegetative phase and delayed flowering. Varietal differences were also observed, particularly for Fibror 79, which flowered slightly later, suggesting greater photoperiod sensitivity. These patterns confirm that both thermal environment and sowing date play a decisive role in hemp phenological development. The findings also highlight the high plasticity of the crop, which demonstrated strong adaptation to the hot and dry Mediterranean summers. Overall, appropriate selection of variety and sowing date can optimize vegetative and reproductive development, representing an important strategy for sustainable agricultural systems in the Alentejo region.