Apple picking is an inherently labor-intensive, time-consuming, and costly task, and robotic harvesting represents a potential alternative to address this challenge. This study presents the development and field evaluation of an integrated robotic system for apple harvesting, which combines machine vision, a dual four-degree-of-freedom (DoF) manipulator, and a mobile platform. The harvesting mechanism employed a streamlined 4-DoF manipulator driven by closed-loop stepper motors, incorporating a differential gear mechanism to execute yaw and pitch motions. Trajectory planning utilized linear interpolation with a harmonic acceleration/deceleration profile to ensure smooth end-effector movement. Fruit detection and localization within the canopy were performed by a stereo vision system running a lightweight deep neural network, achieving a mean hand-eye calibration accuracy of 4.7 ± 2.7 mm. Three negative-pressure driven soft end-effector designs—a suction soft end-effector (SSE), a grasping soft end-effector (GSE), and a suction-grasping soft end-effector (SGSE)—were assessed for their harvesting performance. Field trials conducted in a commercial spindle orchard demonstrated that the GSE achieved the highest performance, with a harvesting success rate of 80.80% among reachable fruits, a full-process success rate (from detection to collection) of 61.59%, an overall fruit damage rate of 10.89%, and an average single-fruit cycle time of 5.27 s. In contrast, the SSE and SGSE showed lower success rates (49.21% and 64.71%, respectively). This work provides a practical robotic harvesting solution. It validates the feasibility of a zoned, multi-manipulator harvesting strategy and delivers comparative data to guide the development of more efficient and robust harvesting robots.