There is increasing evidence showing that moderate amounts of insoluble dietary fiber can improve nutrient utilization by positively influencing the physiology of the gastrointestinal tract. The present study was conducted to investigate the effects of wheat bran as a source of insoluble fiber on nutrient digestibility, serum antioxidant status, gastrointestinal development, digestive enzyme activities and intestinal morphology in broiler chickens. A total of 96 one-day-old male Arbor Acre broiler chickens were assigned to two treatments with six replicate cages per treatment and eight birds per replicate for 42 d. Dietary treatments consisted of the control group (CON, control diet) and wheat bran group (WB, 30 g/kg wheat bran). Inclusion of WB increased (p < 0.05) the digestibility of dry matter, organic matter, gross energy and crude protein on Day 42. Birds fed WB had lower (p < 0.05) serum total cholesterol concentration on Day 21, and lower (p < 0.05) serum concentrations of low-density lipoprotein, total cholesterol and total triglyceride on Day 42. Inclusion of WB increased (p < 0.05) serum glutathione peroxidase activity on Day 21 and superoxide dismutase activity on Day 42, but tended (p = 0.07) to decrease serum malondialdehyde concentration on Day 21, and significantly decreased (p < 0.05) serum malondialdehyde concentration on Day 42. Birds fed WB had a greater (p < 0.05) relative weight of gizzard on both Day 21 and 42. Inclusion of WB increased (p < 0.05) activities of amylase and trypsin in pancreas and jejunal mucosa on Day 21, and increased (p < 0.05) amylase activity in pancreas and jejunal mucosa. Birds fed WB had greater (p < 0.05) villus height and villus height to crypt depth ratio in jejunum and ileum on Day 42. In conclusion, supplementation of 30 g/kg WB enhanced nutrient digestibility by improving antioxidant status, gizzard development, intestinal digestive enzyme activities and morphology of broilers.

This study aimed to investigate the effects of dietary LY or LY combined with ZnO supplementation on performance and gut health in nursery pigs. 192 Duroc × Landrace × Yorkshire piglets (weaned on d 32 of the age with 9.2 ± 1.7 kg BW) were allocated into four treatments with eight replicate pens, six piglets per pen. The treatments included a basal diet as control (CTR), an antibiotic plus ZnO diet (CTC-ZnO, basal diet + 75 mg/kg of chlortetracycline + ZnO (2000 mg/kg from d 1 to 14, 160 mg/kg from d 15 to 28)), a LY diet (LY, basal diet + 2 g/kg LY), and a LY plus ZnO diet (LY-ZnO, basal diet + 1 g/kg LY + ZnO). The results showed that pigs fed LY or LY-ZnO had increased (p < 0.05) average daily gain, serum IgA, IgG, superoxide dismutase, fecal butyric acid, and total volatile fatty acid concentrations, as well as decreased (p < 0.05) feed conversion ratio and diarrhea rate compared with CTR. In conclusion, pigs fed diets with LY or LY combined with ZnO had similar improvement to the use of antibiotics and ZnO in performance, antioxidant status, immunoglobulins, and gut health in nursery pigs.

Maternal diet has a profound impact on growth and immune development of offspring. This study aimed to evaluate the effects of maternal supplementation with a combination of wheat bran (WB, a source of insoluble dietary fiber) and sugar beet pulp (SBP, a source of soluble dietary fiber) on growth and intestinal morphology, immunity, barrier function and microbiota in piglets. Thirty sows (Landrace × Yorkshire; 3–6 parity) were randomly allocated to 2 dietary treatments from d 85 of gestation to weaning (d 21 of lactation). The 2 dietary treatments were: a control diet (CON, a corn–soybean meal diet) and a dietary fiber diet (DF, 15% WB and 10% SBP during gestation and 7.5% WB and 5% SBP during lactation). Maternal DF supplementation improved growth, serum growth hormones and ileal morphology in piglets. Piglets fed DF showed enhanced intestinal barrier function as indicated by reduced serum concentrations of diamine oxidase and endotoxin, and increased ileal mRNA level of occludin. Maternal DF supplementation reduced pro-inflammatory cytokines in the colostrum, milk and serum of piglets. Furthermore, maternal DF supplementation decreased the colonic abundance of Subdoligranulum and Mogibacterium, and increased the colonic abundance of Lactobacillus and norank_f__Bacteroidales_S24-7_group and the colonic concentration of acetate and butyrate in piglets. In summary, maternal supplementation with a combination of SBP and WB during late gestation and lactation improved cytokines in colostrum and milk, growth, immune responses, intestinal morphology, barrier function and microbiota in piglets, which may be a potential strategy to improve offspring growth and intestinal functions.

Introduction: This study was conducted to evaluate the protective effects of inulin against lipopolysaccharide (LPS)-induced oxidative stress in broilers. Methods: A total of 108 1-day-old male Arbor Acres broilers were randomly allocated to 3 treatment groups with 6 replicates per group and 6 birds per replicate. The 3 groups were: (1) non-challenged broilers fed a corn-soybean meal basal diet (control group, CON); (2) LPS-challenged broilers fed the basal diet (LPS); (3) LPS-challenged broilers fed the basal diet supplemented with 15 g/kg inulin (LPS + Inulin). Results: The results showed that LPS administration inhibited activities of antioxidant enzymes and stimulated production of lipid peroxidation in multiple tissues including serum, liver, intestine, and muscle. Dietary inulin supplementation partially alleviated the negative effects of LPS on antioxidant capacity of these tissues, with the underlying mechanism involving the regulation of the Nrf2 signaling pathway. Discussion: Collectively, these findings indicate that inulin supplementation effectively alleviates LPS-induced oxidative stress, highlighting its potential as a feed additive in broiler diets to combat oxidative stress.