Background: Understanding the mechanisms underlying dental pain caused by pulpitis in humans has led to the development of animal models, such as the rat, which enable the study of the mechanisms underlying inflammation; the use of these models is considered ethically justified when the anticipated scientific benefits outweigh the potential impacts on animals in the harm/benefit balance. Objective: To develop a rat model of mechanically induced pulpitis and to evaluate the potential impact on animal well-being. Methods: Pulpitis was mechanically induced in male Lewis rats (13–16 weeks, 350–400 g) which were anesthetized and endotracheally intubated. Following pulp exposure, the cavity was sealed with either amalgam (n = 10) or zinc phosphate cement (n = 10). Following recovery and return to their housing, behavioral assessments and histological evaluations using Hematoxylin and Eosin (H&E) staining were conducted in separate cohorts at two time points: 3 h and 5 days following the procedure. Results: A standardized model of mechanically induced pulpitis was established and verified clinically and by histopathological analysis, which showed evidence of the inflammatory process and revealed no statistically significant differences in the scoring of pain, discomfort, or distress, nor in the measurements of food and water consumption or body weight. Conclusions: The behavioral assessments conducted in this study supported the implementation of a safe and easily reproducible model for future research aimed at elucidating the mechanisms underlying pulp inflammation.

Background: The management of peri‐implant tissue seeks to meet the aesthetic expectations of patients, with a smaller number of procedures that favor the development and maintenance of healthy peri‐implant soft tissue. The customized healing abutment technique on immediate implants has demonstrated numerous functions for the success of restorations on implants. Objectives: The objective of the study is to analyze the biological aspects of the customized healing abutment using PEEK and flowable composite to condition the peri‐implant tissues by presenting a clinical case of a patient from the oral surgery service of Pontificia Xavierian University. Case Report: A patient underwent the placement of two postextraction implants and customized healing abutments in the posteroinferior area. Discussion: This technique is aimed at maintaining the volume of keratinized mucosa and creating an emergence profile for the future prosthesis and reducing the risk of peri‐implantitis. The development of healthy peri‐implant soft tissues is essential to achieve the aesthetics and biological success of implant‐supported restorations in all stages of healing and tissue maturation, and the abutment is essential because it allows a biological seal that protects the bone tissue. Conclusion: It was demonstrated that the use of the customized healing abutment with PEEK and flowable composite immediately after implant placement after dental extraction keeps the volume of the soft and hard tissues around the implants at the time of definitive rehabilitation.

The objective of this study was to examine the efficacy of massive open online courses (MOOCs) for implementing standardized training and teaching with respect to oral cancer among dental students and dentists. In a multicenter interventional randomized controlled trial, a total of 660 Colombian dental students and dentists were randomly assigned to one of three groups between September 2022 and August 2023 by means of the random number table method at ten different institutions involved in dental education. With a loss to follow-up rate of 25%, Group 1 consisted of 107 cases, Group 2 consisted of 177 cases, and the control group consisted of 164 cases. The control group did not receive training, whereas Group 1 received a leaflet, and Group 2 participated in a MOOC-based learning approach. The assessment results of the knowledge, attitudes, and practice scores were observed and compared among the three groups of participants before and 2 months after the intervention. Compared with the control group, Group 2 presented significantly higher scores for KAP ( P < 0.05). The group also showed greater improvements in terms of knowledge, attitudes, and practices ( P < 0.05) than did the control group. Additionally, the KAP scores of the students in Group 2 were significantly higher than those of the dentists. Group 2 also demonstrated a substantially greater size effect in KAP. The implementation of MOOC teaching was shown to positively affect standardized training and teaching related to oral cancer, thereby enhancing academic performance in this area. Trial registration: ISRCTN48708543.

ABSTRACT. Background: Child battered syndrome that affects children and adolescents from all ethnic groups, social classes and religions. In Colombia according to the numbers given by the Colombian Family Welfare Institute of physical abuse affect children between 5-14 years. 75 % of the injuries caused by this syndrome occur to the head or neck region; therefore, the dentist plays an important role in its early detection. Objective: To analyze the correlation between child battering, dental bruxism and clenching in children with and without apparent child abuse of 6 to 18 years. Methods: A cross-sectional descriptive study was made, 104 children (52 children with a previous diagnosis of child abuse and 52 children without this diagnosis), mean age: 6 and 16 years. In both groups a clinical examination was made that included evaluation of habits, muscular system and presence of facets, temporomandibular joint and Test of Beck such as orientation in anxiety levels. Results: the presence of bruxism had no significant relationship between the two groups; while the dental clenching was reflected in a 57.7 % and 32.7 % presented levels of slight or moderate anxiety in battered children. Conclusions: There is a risk factor 16.4 times greater of battered children to present injurious dental clenching and develop temporomandibular disorders in the future.

Background: due of capsaicin effect on the control of various inflammatory mediators, it has been proposed to modulate inflammatory processes caused by physical assaults to pulp tissue. Purpose: to evaluate the effect of capsaicin diluted in 0.05 % ethanol and its vehicle on PGE2 production, IL-8, IL-6, IL-1β, and IL-12p70 in Human Fibroblasts Pulp (FPH). Methods: concentrations of PGE2, IL-8, IL-6, IL-1β, and IL-12p70 were analyzed by ELISA or flow cytometry in supernatants of FPH at 6 and 8 hours, after being stimulated with capsaicin 5*10-5 M and 2,5*10-5 M and ethanol 0.05% vehicle of capsaicin and compared with unstimulated cells. Results: ethanol 0.05% as a diluent capsaicin, is responsible for the effect on the decrease in the expression of PGE2, IL-8 and IL-6 at the times and concentrations evaluated; and IL-12p-70 was the only cytokine that increased significantly in the presence of capsaicin 2,5*10-5 M at 8h, it is statistically greater than ethanol 0.05%. Capsaicin and ethanol had not significant effect on IL-1β. Conclusion: the effect of capsaicin on FPH, is associated with ethanol 0.05% used as diluent and varies depending on time and the mediator analyzed.