Bacteria of the genus Bacillus and related genera (e.g., Paenibacillus, Alicyclobacillus or Brevibacillus) belong to the phylum Firmicutes. Taxonomically, it is a diverse group of bacteria that, to date, has not been well described phylogenetically. The group consists of aerobic and relatively anaerobic bacteria, capable of spore-forming. Bacillus spp. and related genera are widely distributed in the environment, with a particular role in soil. Their abundance in the agricultural environment depends mainly on fertilization, but can also depend on soil cultivated methods, meaning whether the plants are grown in monoculture or rotation systems. The highest abundance of the phylum Firmicutes is usually recorded in soil fertilized with manure. Due to the great abundance of cellulose in the environment, one of the most important physiological groups among these spore-forming bacteria are cellulolytic bacteria. Three key cellulases produced by Bacillus spp. and related genera are required for complete cellulose degradation and include endoglucanases, exoglucanases, and β-glucosidases. Due to probable independent evolution, cellulases are encoded by hundreds of genes, which results in a large structural diversity of these enzymes. The microbial degradation of cellulose depends on its type and environmental conditions such as pH, temperature, and various substances including metal ions. In addition, Bacillus spp. are among a few bacteria capable of producing multi-enzymatic protein complexes called cellulosomes. In conclusion, the taxonomy of Bacillus spp. and related bacteria needs to be reorganized based on, among other things, additional genetic markers. Also, the ecology of soil bacteria of the genus Bacillus requires additions, especially in the identification of physical and chemical parameters affecting the occurrence of the group of bacteria. Finally, it is worth adding that despite many spore-forming strains well-studied for cellulolytic activity, still few are used in industry, for instance for biodegradation or bioconversion of lignocellulosic waste into biogas or biofuel. Therefore, research aimed at optimizing the cellulolytic properties of spore-forming bacteria is needed for more efficient commercialization.

Although soil water repellency (SWR) has been reported under different soils, climates, and vegetation types of the world, especially in forest land and following wildfires, the understanding of this variable continues to be rather limited. This study presented the characterization of SWR from wild fire measurements in a Scots pine Peucedano-Pinetum forest in the Kampinos National Park (central Poland), which is characterized by a temperate continental climate. The main objectives were: [i] To evaluate the potential occurrence, intensity, and persistence of soil water repellency in the surface layers of podzolized rusty soils during a dry summer; [ii] to determine whether a wildfire increased SWR, compared to the unburnt condition of soil; and [iii] to identify changes in hydrophobicity 13 months after a fire. The Water Drop Penetration Time (WDPT) test was used to assess persistence and intensity of soil SWR. Hydrophobicity is a natural phenomenon during periods of drought in temperate continental climates. The extreme class of SWR was observed in surface layers of up to 20 cm. A higher hydrophobicity was noted in the older habitats of the Peucedano-Pinetum forest. Maximum WDPT values (10,800 s) were found for an older ecosystem cover, during a dry summer. SWR in fire-affected soils is dependent on the intensity of the fire, as well as displaying spatial and seasonal variability. Thirteen months after a fire, the highest variability in the occurrence of non-wettability, was recorded in the surface layers of areas affected by a weak fire. A positive relationship between soil pH and WDPT values was determined to a 20 cm depth. Prolonged dry periods resulting from global climate change, may enhance the effects of increasing SWR; it therefore seems reasonable for future research on biosphere–climate interactions, to take the presence of hydrophobicity into account.

The microbial activity of soil enriched with sewage sludge and cultivated with energy crops, is little known. The aim of this study was to assess the impact of sewage sludge fertilization on selected microorganisms in soil cultivated with the following crops: Miscanthus (Miscanthus x giganteus Greef et Deu), Virginia mallow (Sida hermaphrodita (L.) Rusby) and Common Osier willow (Salix viminalis L.). Sewage sludge was used in two rates 10 and 20 t/ha dry weight (DW). The numbers of total coliforms bacteria, sulphate-reducing spore-forming bacteria, Proteus sp., saprophytic, thermophilic and aerobic spore-forming bacteria were examined. Sewage sludge increased the number of coliforms and sulphate-reducing spore-forming bacteria, and stimulated the growth of saprophytic and thermophilic bacteria. Cultivation of Miscanthus limited the number of coliforms bacteria, while Virginia mallow and Miscanthus reduced the number of sulphate- -reducing spore-forming bacteria. Common Osier willow stimulated the growth of saprophytic bacteria in the soil, while Virginia mallow the number of spore-forming bacteria. Our results revealed that microbial activity of soil expressed as the number of selected bacterial groups, depends not only on the applied rate of sewage sludge fertilizer, but also on the cultivated energy crop.