The paired auditory organ of mosquito, the Johnston's organ (JO), being the receiver of particle velocity component of sound, is directional by its structure. However, to date almost no physiological measurements of its directionality was done. In addition, the recent finding on the grouping of the JO auditory neurons into the antiphase pairs demanded confirmation by different methods. Using the vector superposition of the signals produced by two orthogonally oriented speakers, we measured the directional characteristics of individual units as well as their relations in physiologically distinguishable groups – pairs or triplets. The feedback stimulation method allowed to discriminate responses of the two simultaneously recorded units, and to show that they indeed responded in antiphase. Units of different frequency tuning as well as high-sensitive units (thresholds of 27 dB SPVL and below) were found in every angular sector of the JO, providing the mosquito with the ability to produce complex auditory behaviors.

We experimentally demonstrated that tonal acoustic signals with a carrier frequency of 140–200 Hz had a repellent effect on male mosquitoes (Culicidae). Swarming males of Aedes diantaeuswere...

The task of directional hearing faces most animals that possess ears. They approach this task in different ways, but a common trait is the use of binaural cues to find the direction to the source of sound. In insects, the task is further complicated by their small size and, hence, minute temporal and level differences between two ears. A single symmetric flagellar particle velocity receiver, such as the antenna of a mosquito, should not be able to discriminate between the two opposite directions along the vector of the sound wave. Paired antennae of mosquitoes presume the usage of binaural hearing, but its mechanisms are expected to be significantly different from the ones typical for the pressure receivers. However, the directionality of flagellar auditory organs has received little attention. Here, we measured the in-flight orientation of antennae in female Culex pipiens pipiens mosquitoes and obtained a detailed physiological mapping of the Johnston’s organ directionality at the level of individual sensory units. By combining these data, we created a three-dimensional model of the mosquito’s auditory space. The orientation of the antennae was found to be coordinated with the neuronal asymmetry of the Johnston’s organs to maintain a uniformly shaped auditory space, symmetric relative to a flying mosquito. The overlap of the directional characteristics of the left and right sensory units was found to be optimal for binaural hearing focused primarily in front of, above and below a flying mosquito.