Humanized mouse models offer human-specific platforms for investigating complex host–pathogen interactions, addressing shortcomings of conventional preclinical models that often fail to replicate human immune responses accurately. This integrative review examines the intersection of advanced morphological phenotyping and artificial intelligence (AI) to enhance predictive capacity and translational relevance in infectious disease research. A structured literature search was conducted across PubMed, Scopus, and Web of Science (2010–2025), applying defined inclusion and exclusion criteria. Evidence synthesis highlights imaging modalities, AI-driven phenotyping, and standardization strategies, supported by comparative analyses and quality considerations. Persistent challenges include variability in engraftment, lack of harmonized scoring systems, and ethical governance. We propose recommendations for standardized protocols, risk-of-bias mitigation, and collaborative training frameworks to accelerate adoption of these technologies in translational medicine.

MPs are pervasive pollutants in marine ecosystems, posing risks to aquatic organisms due to their small size and bioaccumulation potential. This study investigated the intestinal toxicity of MP particles extracted from mangrove sediments in zebrafish, comparing the effects before and after microbial degradation. Zebrafish were exposed to either undegraded MPs or microbially degraded MP extracts at concentrations of 0 (control), 2, 10, and 50 mg/L for 21 days in 10 L tanks (stocking density: 10 fish/L), with three replicate tanks per concentration. MPs were dispersed ultrasonically before addition to the water. Intestinal samples were collected on 7, 14, and 21 days for the analysis of immune response (tumor necrosis factor-alpha, TNF-α; interleukin-1 beta, IL-1β; interleukin-6, IL-6; interleukin-8, IL-8) and antioxidant activity (superoxide dismutase, SOD; catalase, CAT). Histopathological analysis revealed intestinal wall thinning, villus damage, and epithelial cell detachment in zebrafish exposed to both undegraded and degraded MP extracts; however, undegraded MPs induced more severe intestinal damage. Results indicated dynamic changes in cytokine expression: TNF-α decreased initially before increasing, while IL-1β and IL-8 first rose then declined. IL-6 peaked on day 7, dropped by day 14, and increased again on day 21. CAT expression decreased, whereas SOD increased only in the pre-degradation group. Microbial degradation reduced intestinal damage severity, with effects intensifying at higher MP exposure levels. These findings demonstrate that MPs can impair zebrafish digestive systems, but microbial degradation mitigates their toxicity. This study underscores the importance of biodegradation as a potential environmental remediation strategy and provides experimental evidence on MPs’ impact on aquatic organisms.

The rabbit industry urgently needs natural alternatives to maintain gut health. This need is growing due to the higher incidence of intestinal problems in antibiotic-free production systems. Pfaffia glomerata (Brazilian ginseng) is a medicinal plant rich in bioactive compounds. This study evaluated the effects of dietary inclusion of P. glomerata leaf powder on growth performance, immune function, and gut microbiota in New Zealand rabbits. A total of 100 New Zealand rabbits (35 days old) were randomly assigned to five groups (n = 20 each). The groups comprised a blank control group (CON), an immunosuppressed group (CTX), and three treatment groups receiving low (L), medium (M), and high (H) doses of P. glomerata leaf powder at 0.5%, 1%, and 2% of the basal diet, respectively. Growth performance was assessed by average daily gain (ADG). Intestinal morphology was evaluated by measuring villus height and crypt depth in the duodenum, jejunum, and ileum. Immune parameters included thymus and spleen weight, serum immunoglobulin levels, and leukocyte counts. Cecal microbiota diversity and composition were analyzed. Compared to CON, Pfaffia supplementation improved growth performance: the L and H groups had significantly higher ADG and a lower feed conversion ratio (p < 0.05). In intestinal histology, groups L and M had reduced crypt depth in the duodenum (p < 0.05), and group L had an increased villus height to crypt depth ratio. In the jejunum and ileum, groups L and H exhibited increased villus height and villus height to crypt depth ratio (p < 0.05), indicating an enhanced absorptive surface. In the immunosuppressed model, rabbits in all Pfaffia groups showed significantly increased thymus and spleen weight compared to the CTX group (p < 0.05) and demonstrated elevated serum immunoglobulins and leukocyte counts (monocytes, lymphocytes, and neutrophils) (p < 0.05). Pfaffia supplementation also enhanced cecal microbiota diversity and increased the abundance of beneficial bacteria. In summary, dietary P. glomerata leaf powder enhanced growth performance, immune organ development, and healthy gut microbiota in growing rabbits. The 0.5% supplementation level (L group) yielded the most consistent benefits. These results suggest that P. glomerata is a promising natural feed additive to promote rabbit health and production in antibiotic-free systems.