The aim of this study was to compare the aqueous humor (AH) and serum concentrations of metabolites in diabetic (n = 36) and nondiabetic (n = 36) senior adults undergoing cataract surgery. Blood samples were collected before surgery and AH during surgery. Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS)-based targeted metabolomic and lipidomic analyses of samples were performed using the AbsoluteIDQ^® p180 kit. Out of 188 metabolites targeted by the kit, 41 and 133 were detected in >80% of AH and serum samples, respectively. Statistical analysis performed to indicate metabolites differentiating diabetic and nondiabetic patients showed 8 and 20 significant metabolites in AH and serum, respectively. Pathway analysis performed for significant metabolites revealed that galactose metabolism is mostly affected in the AH, while arginine biosynthesis is mostly affected in the serum. Among metabolites that differentiate diabetic and nondiabetic patients, arginine was the only metabolite common to both serum and AH samples, as well as the only one with a decreased concentration in both body fluids of diabetic patients. Concentrations of the rest were elevated in AH and lowered in serum. This may suggest different mechanisms of diabetes-related dysregulation of the local metabolism in the eye in comparison to systemic changes observed in the blood.

This study aims to evaluate aqueous humor (AH) and serum metabolite concentrations in myopic and control adults undergoing cataract surgery and to correlate them with axial length (AL) to gain a better insight into the pathogenesis at both the local and systemic levels. Ninety-three patients were assigned to the myopic (n = 52) and control (n = 41) groups. Venous blood and aqueous humor samples were collected and analyzed by means of targeted metabolomics based on liquid chromatography-tandem mass spectrometry. The univariate analysis has revealed distinct metabolomic profiles between the myopic and control groups in AH but not in serum. In the AH of myopic patients, symmetric dimethylarginine (SDMA) and taurine concentrations were significantly lower. SDMA and taurine levels in the AH correlated negatively with the AL. These findings account for the insight into the local metabolic alterations in the case of myopia, potentially leading to novel therapeutic targets.