This page has only limited features, please log in for full access.
A crucial step in nonintrusive load monitoring (NILM) is feature extraction, which consists of signal processing techniques to extract features from voltage and current signals. This paper presents a new time-frequency feature based on Stockwell transform. The extracted features aim to describe the shape of the current transient signal by applying an energy measure on the fundamental and the harmonic frequency voices. In order to validate the proposed methodology, classical machine learning tools are applied (k-NN and decision tree classifiers) on two existing datasets (Controlled On/Off Loads Library (COOLL) and Home Equipment Laboratory Dataset (HELD1)). The classification rates achieved are clearly higher than that for other related studies in the literature, with 99.52% and 96.92% classification rates for the COOLL and HELD1 datasets, respectively.
Mahfoud Drouaz; Bruno Colicchio; Ali Moukadem; Alain Dieterlen; Djafar Ould-Abdeslam. New Time-Frequency Transient Features for Nonintrusive Load Monitoring. Energies 2021, 14, 1437 .
AMA StyleMahfoud Drouaz, Bruno Colicchio, Ali Moukadem, Alain Dieterlen, Djafar Ould-Abdeslam. New Time-Frequency Transient Features for Nonintrusive Load Monitoring. Energies. 2021; 14 (5):1437.
Chicago/Turabian StyleMahfoud Drouaz; Bruno Colicchio; Ali Moukadem; Alain Dieterlen; Djafar Ould-Abdeslam. 2021. "New Time-Frequency Transient Features for Nonintrusive Load Monitoring." Energies 14, no. 5: 1437.
In this paper, we study the solving of the gridless sparse optimization problem and its application to 3D image deconvolution. Based on the recent works of (Denoyelle et al., 2019) introducing the Sliding Frank-Wolfe algorithm to solve the Beurling LASSO problem, we introduce an accelerated algorithm, denoted BSFW, that preserves its convergence properties, while removing most of the costly local descents. Besides, as the solving of BLASSO still relies on a regularization parameter, we introduce a homotopy algorithm to solve the constrained BLASSO that allows to use a more practical parameter based on the image residual, e.g. its standard deviation. Both algorithms benefit from a finite termination property, i.e. they are guaranteed to find the solution in a finite number of step under mild conditions. These methods are then applied on the problem of 3D tomographic diffractive microscopy images, with the purpose of explaining the image by a small number of atoms in convolved observations. Numerical results on synthetic and real images illustrates the improvement provided by the BSFW method, the homotopy method and their combination.
Jean-Baptiste Courbot; Bruno Colicchio. A fast homotopy algorithm for gridless sparse recovery. Inverse Problems 2020, 37, 025002 .
AMA StyleJean-Baptiste Courbot, Bruno Colicchio. A fast homotopy algorithm for gridless sparse recovery. Inverse Problems. 2020; 37 (2):025002.
Chicago/Turabian StyleJean-Baptiste Courbot; Bruno Colicchio. 2020. "A fast homotopy algorithm for gridless sparse recovery." Inverse Problems 37, no. 2: 025002.
The importance of looking into microgrid security is getting more crucial due to the cyber vulnerabilities introduced by digitalization and the increasing dependency on information and communication technology (ICT) systems. Especially with a current academic unanimity on the incremental significance of the microgrid’s role in building the future smart grid, this article addresses the existing approaches attending to cyber-physical security in power systems from a microgrid-oriented perspective. First, we start with a brief descriptive review of the most commonly used terms in the latest relevant literature, followed by a comprehensive presentation of the recent efforts explored in a manner that helps the reader to choose the appropriate future research direction among several fields.
Bushra Canaan; Bruno Colicchio; Djaffar Ould Abdeslam. Microgrid Cyber-Security: Review and Challenges toward Resilience. Applied Sciences 2020, 10, 5649 .
AMA StyleBushra Canaan, Bruno Colicchio, Djaffar Ould Abdeslam. Microgrid Cyber-Security: Review and Challenges toward Resilience. Applied Sciences. 2020; 10 (16):5649.
Chicago/Turabian StyleBushra Canaan; Bruno Colicchio; Djaffar Ould Abdeslam. 2020. "Microgrid Cyber-Security: Review and Challenges toward Resilience." Applied Sciences 10, no. 16: 5649.
Dicentric chromosomes are a relevant marker of chromosomal instability. Their appearance is associated with telomere dysfunction, leading to cancer progression and a poor clinical outcome. Here, we present Telomere and Centromere staining followed by M-FISH (TC+M-FISH) for improved detection of telomere dysfunction and the identification of dicentric chromosomes in cancer patients and various genetic syndromes. Significant telomere length shortening and significantly higher frequencies of telomere loss and deletion were found in the peripheral lymphocytes of patients with cancer and genetic syndromes relative to similar age-matched healthy donors. We assessed our technique against conventional cytogenetics for the detection of dicentric chromosomes by subjecting metaphase preparations to both approaches. We identified dicentric chromosomes in 28/50 cancer patients and 21/44 genetic syndrome patients using our approach, but only 7/50 and 12/44, respectively, using standard cytogenetics. We ascribe this discrepancy to the identification of the unique configuration of dicentric chromosomes. We observed significantly higher frequencies of telomere loss and deletion in patients with dicentric chromosomes (p < 10−4). TC+M-FISH analysis is superior to classical cytogenetics for the detection of chromosomal instability. Our approach is a relatively simple but useful tool for documenting telomere dysfunction and chromosomal instability with the potential to become a standard additional diagnostic tool in medical genetics and the clinic.
Radhia M’Kacher; Bruno Colicchio; Claire Borie; Steffen Junker; Valentine Marquet; Leonhard Heidingsfelder; Kevin Soehnlen; Wala Najar; William M. Hempel; Noufissa Oudrhiri; Nadège Wilhelm-Murer; Marguerite Miguet; Micheline Arnoux; Catherine Ferrapie; Wendy Kerbrat; Andreas Plesch; Alain Dieterlen; Theodore Girinsky; Philippe Voisin; Georges Deschenes; Anne-Claude Tabet; Catherine Yardin; Annelise Bennaceur-Griscelli; Michael Fenech; Patrice Carde; Eric Jeandidier. Telomere and Centromere Staining Followed by M-FISH Improves Diagnosis of Chromosomal Instability and Its Clinical Utility. Genes 2020, 11, 475 .
AMA StyleRadhia M’Kacher, Bruno Colicchio, Claire Borie, Steffen Junker, Valentine Marquet, Leonhard Heidingsfelder, Kevin Soehnlen, Wala Najar, William M. Hempel, Noufissa Oudrhiri, Nadège Wilhelm-Murer, Marguerite Miguet, Micheline Arnoux, Catherine Ferrapie, Wendy Kerbrat, Andreas Plesch, Alain Dieterlen, Theodore Girinsky, Philippe Voisin, Georges Deschenes, Anne-Claude Tabet, Catherine Yardin, Annelise Bennaceur-Griscelli, Michael Fenech, Patrice Carde, Eric Jeandidier. Telomere and Centromere Staining Followed by M-FISH Improves Diagnosis of Chromosomal Instability and Its Clinical Utility. Genes. 2020; 11 (5):475.
Chicago/Turabian StyleRadhia M’Kacher; Bruno Colicchio; Claire Borie; Steffen Junker; Valentine Marquet; Leonhard Heidingsfelder; Kevin Soehnlen; Wala Najar; William M. Hempel; Noufissa Oudrhiri; Nadège Wilhelm-Murer; Marguerite Miguet; Micheline Arnoux; Catherine Ferrapie; Wendy Kerbrat; Andreas Plesch; Alain Dieterlen; Theodore Girinsky; Philippe Voisin; Georges Deschenes; Anne-Claude Tabet; Catherine Yardin; Annelise Bennaceur-Griscelli; Michael Fenech; Patrice Carde; Eric Jeandidier. 2020. "Telomere and Centromere Staining Followed by M-FISH Improves Diagnosis of Chromosomal Instability and Its Clinical Utility." Genes 11, no. 5: 475.
A link between telomere shortening and oxidative stress was found in aging people and patients with cancer or inflammatory diseases. Extracts of Astragalus spp. are known to stimulate telomerase activity, thereby compensating telomere shortening. We characterized a multi-component hydroethanolic root extract (HRE) of Astragalus mongholicus Bunge and assessed its effects on telomeres compared to those of danazol. Astragalosides I to IV, flavonoids, amino acids and sugars were detected in the HRE. Samples of peripheral blood lymphocytes with short telomeres from 18 healthy donors (mean age 63.5 years; range 32–86 years) were exposed to a single dose of 1 µg/mL HRE or danazol for three days. Telomere length and telomerase expression were then measured. Significant elongation of telomeres associated to a less toxicity was observed in lymphocytes from 13/18 donors following HRE treatment (0.54 kb (0.15–2.06 kb)) and in those from 9/18 donors after danazol treatment (0.95 kb (0.06–2.06 kb)). The rate of cells with short telomeres (
Isabelle Guinobert; Claude Blondeau; Bruno Colicchio; Noufissa Oudrhiri; Alain Dieterlen; Eric Jeandidier; Georges Deschenes; Valérie Bardot; César Cotte; Isabelle Ripoche; Patrice Carde; Lucile Berthomier; Radhia M’Kacher. The Use of Natural Agents to Counteract Telomere Shortening: Effects of a Multi-Component Extract of Astragalus mongholicus Bunge and Danazol. Biomedicines 2020, 8, 31 .
AMA StyleIsabelle Guinobert, Claude Blondeau, Bruno Colicchio, Noufissa Oudrhiri, Alain Dieterlen, Eric Jeandidier, Georges Deschenes, Valérie Bardot, César Cotte, Isabelle Ripoche, Patrice Carde, Lucile Berthomier, Radhia M’Kacher. The Use of Natural Agents to Counteract Telomere Shortening: Effects of a Multi-Component Extract of Astragalus mongholicus Bunge and Danazol. Biomedicines. 2020; 8 (2):31.
Chicago/Turabian StyleIsabelle Guinobert; Claude Blondeau; Bruno Colicchio; Noufissa Oudrhiri; Alain Dieterlen; Eric Jeandidier; Georges Deschenes; Valérie Bardot; César Cotte; Isabelle Ripoche; Patrice Carde; Lucile Berthomier; Radhia M’Kacher. 2020. "The Use of Natural Agents to Counteract Telomere Shortening: Effects of a Multi-Component Extract of Astragalus mongholicus Bunge and Danazol." Biomedicines 8, no. 2: 31.
The authors wish to make the following corrections to this paper
Corina Cuceu; Bruno Colicchio; Eric Jeandidier; Steffen Junker; François Plassa; Grace Shim; Justyna Mika; Monika Frenzel; Mustafa Al Al Jawhari; William M. Hempel; Sylwia Kabacik; Aude Lenain; Luc Morat; Theodore Girinsky; Alain Dieterlen; Joanna Polanska; Christophe Badie; Patrice Carde; Radhia M'kacher; Mustafa Al Jawhari. Erratum: Cuceu, C., et al. Independent Mechanisms Lead to Genomic Instability in Hodgkin Lymphoma: Microsatellite or Chromosomal Instability. Cancers 2018, 10, 233. Cancers 2019, 11, 757 .
AMA StyleCorina Cuceu, Bruno Colicchio, Eric Jeandidier, Steffen Junker, François Plassa, Grace Shim, Justyna Mika, Monika Frenzel, Mustafa Al Al Jawhari, William M. Hempel, Sylwia Kabacik, Aude Lenain, Luc Morat, Theodore Girinsky, Alain Dieterlen, Joanna Polanska, Christophe Badie, Patrice Carde, Radhia M'kacher, Mustafa Al Jawhari. Erratum: Cuceu, C., et al. Independent Mechanisms Lead to Genomic Instability in Hodgkin Lymphoma: Microsatellite or Chromosomal Instability. Cancers 2018, 10, 233. Cancers. 2019; 11 (6):757.
Chicago/Turabian StyleCorina Cuceu; Bruno Colicchio; Eric Jeandidier; Steffen Junker; François Plassa; Grace Shim; Justyna Mika; Monika Frenzel; Mustafa Al Al Jawhari; William M. Hempel; Sylwia Kabacik; Aude Lenain; Luc Morat; Theodore Girinsky; Alain Dieterlen; Joanna Polanska; Christophe Badie; Patrice Carde; Radhia M'kacher; Mustafa Al Jawhari. 2019. "Erratum: Cuceu, C., et al. Independent Mechanisms Lead to Genomic Instability in Hodgkin Lymphoma: Microsatellite or Chromosomal Instability. Cancers 2018, 10, 233." Cancers 11, no. 6: 757.
To identify the cells responsible for the initiation and maintenance of Hodgkin lymphoma (HL) cells, we have characterized a subpopulation of HL cells grown in vitro and in vivo with the aim of establishing a reliable and robust animal model for HL. To validate our model, we challenged the tumor cells in vivo by injecting the alkylating histone-deacetylase inhibitor, EDO-S101, a salvage regimen for HL patients, into xenografted mice. Methodology: Blood lymphocytes from 50 HL patients and seven HL cell lines were used. Immunohistochemistry, flow cytometry, and cytogenetics analyses were performed. The in vitro and in vivo effects of EDO-S101 were assessed. Results: We have successfully determined conditions for in vitro amplification and characterization of the HL L428-c subline, containing a higher proportion of CD30−/CD15− cells than the parental L428 cell line. This subline displayed excellent clonogenic potential and reliable reproducibility upon xenografting into immunodeficient NOD-SCID-gamma (−/−)(NSG) mice. Using cell sorting, we demonstrate that CD30−/CD15− subpopulations can gain the phenotype of the L428-c cell line in vitro. Moreover, the human cells recovered from the seventh week after injection of L428-c cells into NSG mice were small cells characterized by a high frequency of CD30−/CD15− cells. Cytogenetic analysis demonstrated that they were diploid and showed high telomere instability and telomerase activity. Accordingly, chromosomal instability emerged, as shown by the formation of dicentric chromosomes, ring chromosomes, and breakage/fusion/bridge cycles. Similarly, high telomerase activity and telomere instability were detected in circulating lymphocytes from HL patients. The beneficial effect of the histone-deacetylase inhibitor EDO-S101 as an anti-tumor drug validated our animal model. Conclusion: Our HL animal model requires only 103 cells and is characterized by a high survival/toxicity ratio and high reproducibility. Moreover, the cells that engraft in mice are characterized by a high frequency of small CD30−/CD15− cells exhibiting high telomerase activity and telomere dysfunction.
Radhia M’Kacher; Monika Frenzel; Mustafa Al Jawhari; Steffen Junker; Corina Cuceu; Luc Morat; Anne-Laure Bauchet; Lev Stimmer; Aude Lenain; Nathalie Dechamps; William M. Hempel; Geraldine Pottier; Leonhard Heidingsfelder; Eric Laplagne; Claire Borie; Noufissa Oudrhiri; Dima Jouni; Annelise Bennaceur-Griscelli; Bruno Colicchio; Alain Dieterlen; Theodore Girinsky; Raphael Boisgard; Jean Bourhis; Jacques Bosq; Thomas Mehrling; Eric Jeandidier; Patrice Carde. Establishment and Characterization of a Reliable Xenograft Model of Hodgkin Lymphoma Suitable for the Study of Tumor Origin and the Design of New Therapies. Cancers 2018, 10, 414 .
AMA StyleRadhia M’Kacher, Monika Frenzel, Mustafa Al Jawhari, Steffen Junker, Corina Cuceu, Luc Morat, Anne-Laure Bauchet, Lev Stimmer, Aude Lenain, Nathalie Dechamps, William M. Hempel, Geraldine Pottier, Leonhard Heidingsfelder, Eric Laplagne, Claire Borie, Noufissa Oudrhiri, Dima Jouni, Annelise Bennaceur-Griscelli, Bruno Colicchio, Alain Dieterlen, Theodore Girinsky, Raphael Boisgard, Jean Bourhis, Jacques Bosq, Thomas Mehrling, Eric Jeandidier, Patrice Carde. Establishment and Characterization of a Reliable Xenograft Model of Hodgkin Lymphoma Suitable for the Study of Tumor Origin and the Design of New Therapies. Cancers. 2018; 10 (11):414.
Chicago/Turabian StyleRadhia M’Kacher; Monika Frenzel; Mustafa Al Jawhari; Steffen Junker; Corina Cuceu; Luc Morat; Anne-Laure Bauchet; Lev Stimmer; Aude Lenain; Nathalie Dechamps; William M. Hempel; Geraldine Pottier; Leonhard Heidingsfelder; Eric Laplagne; Claire Borie; Noufissa Oudrhiri; Dima Jouni; Annelise Bennaceur-Griscelli; Bruno Colicchio; Alain Dieterlen; Theodore Girinsky; Raphael Boisgard; Jean Bourhis; Jacques Bosq; Thomas Mehrling; Eric Jeandidier; Patrice Carde. 2018. "Establishment and Characterization of a Reliable Xenograft Model of Hodgkin Lymphoma Suitable for the Study of Tumor Origin and the Design of New Therapies." Cancers 10, no. 11: 414.
Background: Microsatellite and chromosomal instability have been investigated in Hodgkin lymphoma (HL). Materials and Methods: We studied seven HL cell lines (five Nodular Sclerosis (NS) and two Mixed Cellularity (MC)) and patient peripheral blood lymphocytes (100 NS-HL and 23 MC-HL). Microsatellite instability (MSI) was assessed by PCR. Chromosomal instability and telomere dysfunction were investigated by FISH. DNA repair mechanisms were studied by transcriptomic and molecular approaches. Results: In the cell lines, we observed high MSI in L428 (4/5), KMH2, and HDLM2 (3/5), low MSI in L540, L591, and SUP-HD1, and none in L1236. NS-HL cell lines showed telomere shortening, associated with alterations of nuclear shape. Small cells were characterized by telomere loss and deletion, leading to chromosomal fusion, large nucleoplasmic bridges, and breakage/fusion/bridge (B/F/B) cycles, leading to chromosomal instability. The MC-HL cell lines showed substantial heterogeneity of telomere length. Intrachromosmal double strand breaks induced dicentric chromosome formation, high levels of micronucleus formation, and small nucleoplasmic bridges. B/F/B cycles induced complex chromosomal rearrangements. We observed a similar pattern in circulating lymphocytes of NS-HL and MC-HL patients. Transcriptome analysis confirmed the differences in the DNA repair pathways between the NS and MC cell lines. In addition, the NS-HL cell lines were radiosensitive and the MC-cell lines resistant to apoptosis after radiation exposure. Conclusions: In mononuclear NS-HL cells, loss of telomere integrity may present the first step in the ongoing process of chromosomal instability. Here, we identified, MSI as an additional mechanism for genomic instability in HL.
Corina Cuceu; Bruno Colicchio; Eric Jeandidier; Steffen Junker; François Plassa; Grace Shim; Justyna Mika; Monika Frenzel; Mustafa Al Jawhari; William M. Hempel; Sylwia Kabacik; Aude Lenain; Luc Morat; Theodore Girinsky; Alain Dieterlen; Joanna Polanska; Christophe Badie; Patrice Carde; Radhia M’Kacher. Independent Mechanisms Lead to Genomic Instability in Hodgkin Lymphoma: Microsatellite or Chromosomal Instability. Cancers 2018, 10, 233 .
AMA StyleCorina Cuceu, Bruno Colicchio, Eric Jeandidier, Steffen Junker, François Plassa, Grace Shim, Justyna Mika, Monika Frenzel, Mustafa Al Jawhari, William M. Hempel, Sylwia Kabacik, Aude Lenain, Luc Morat, Theodore Girinsky, Alain Dieterlen, Joanna Polanska, Christophe Badie, Patrice Carde, Radhia M’Kacher. Independent Mechanisms Lead to Genomic Instability in Hodgkin Lymphoma: Microsatellite or Chromosomal Instability. Cancers. 2018; 10 (7):233.
Chicago/Turabian StyleCorina Cuceu; Bruno Colicchio; Eric Jeandidier; Steffen Junker; François Plassa; Grace Shim; Justyna Mika; Monika Frenzel; Mustafa Al Jawhari; William M. Hempel; Sylwia Kabacik; Aude Lenain; Luc Morat; Theodore Girinsky; Alain Dieterlen; Joanna Polanska; Christophe Badie; Patrice Carde; Radhia M’Kacher. 2018. "Independent Mechanisms Lead to Genomic Instability in Hodgkin Lymphoma: Microsatellite or Chromosomal Instability." Cancers 10, no. 7: 233.
Background: We analyzed telomere maintenance mechanisms (TMMs) in lymph node samples from HL patients treated with standard therapy. The TMMs correlated with clinical outcomes of patients. Materials and Methods: Lymph node biopsies obtained from 38 HL patients and 24 patients with lymphadenitis were included in this study. Seven HL cell lines were used as in vitro models. Telomerase activity (TA) was assessed by TRAP assay and verified through hTERT immunofluorescence expression; alternative telomere lengthening (ALT) was also assessed, along with EBV status. Results: Both TA and ALT mechanisms were present in HL lymph nodes. Our findings were reproduced in HL cell lines. The highest levels of TA were expressed in CD30−/CD15− cells. Small cells were identified with ALT and TA. Hodgkin and Reed Sternberg cells contained high levels of PML bodies, but had very low hTERT expression. There was a significant correlation between overall survival (p < 10−3), event-free survival (p < 10−4), and freedom from progression (p < 10−3) and the presence of an ALT profile in lymph nodes of EBV+ patients. Conclusion: The presence of both types of TMMs in HL lymph nodes and in HL cell lines has not previously been reported. TMMs correlate with the treatment outcome of EBV+ HL patients.
Radhia M’Kacher; Corina Cuceu; Mustafa Al Jawhari; Luc Morat; Monika Frenzel; Grace Shim; Aude Lenain; William M. Hempel; Steffen Junker; Théodore Girinsky; Bruno Colicchio; Alain Dieterlen; Leonhard Heidingsfelder; Claire Borie; Noufissa Oudrhiri; Annelise Bennaceur-Griscelli; Olivier Morales; Sarah Renaud; Zoe Van De Wyngaert; Eric Jeandidier; Nadira Delhem; Patrice Carde. The Transition between Telomerase and ALT Mechanisms in Hodgkin Lymphoma and Its Predictive Value in Clinical Outcomes. Cancers 2018, 10, 169 .
AMA StyleRadhia M’Kacher, Corina Cuceu, Mustafa Al Jawhari, Luc Morat, Monika Frenzel, Grace Shim, Aude Lenain, William M. Hempel, Steffen Junker, Théodore Girinsky, Bruno Colicchio, Alain Dieterlen, Leonhard Heidingsfelder, Claire Borie, Noufissa Oudrhiri, Annelise Bennaceur-Griscelli, Olivier Morales, Sarah Renaud, Zoe Van De Wyngaert, Eric Jeandidier, Nadira Delhem, Patrice Carde. The Transition between Telomerase and ALT Mechanisms in Hodgkin Lymphoma and Its Predictive Value in Clinical Outcomes. Cancers. 2018; 10 (6):169.
Chicago/Turabian StyleRadhia M’Kacher; Corina Cuceu; Mustafa Al Jawhari; Luc Morat; Monika Frenzel; Grace Shim; Aude Lenain; William M. Hempel; Steffen Junker; Théodore Girinsky; Bruno Colicchio; Alain Dieterlen; Leonhard Heidingsfelder; Claire Borie; Noufissa Oudrhiri; Annelise Bennaceur-Griscelli; Olivier Morales; Sarah Renaud; Zoe Van De Wyngaert; Eric Jeandidier; Nadira Delhem; Patrice Carde. 2018. "The Transition between Telomerase and ALT Mechanisms in Hodgkin Lymphoma and Its Predictive Value in Clinical Outcomes." Cancers 10, no. 6: 169.
International audiencePremature chromosome condensation (PCC) enables the direct observation of cytogenetic damage in nonstimulated human interphase peripheral blood lymphocytes. Conventional uniform staining of PCC fusions does not allow the identification of dicentrics, the most important biomarkers for recent exposure to radiation.We have combined telomere and centromere staining of PCC fusions to identify dicentrics, making it possible to establish the first dicentrics radiation doseeresponse curve for a PCC fusion. This improvement permits the automation of the process. This new approach can be used for biological dosimetry in radiation emergency medicine and also for patient follow-up where the rapid and accurate estimation of the dose of genotoxic agents is considered to be a high priority
Radhia M’Kachera; Elie El Maalouf; Georgia Terzoudi; Michelle Ricoul; Leonhard Heidingsfelder; Ionna Karachristou; Eric Laplagne; William M. Hempel; Bruno Colicchio; Alain Dieterlen; Gabriel Pantelias; Laure Sabatier. Detection and Automated Scoring of Dicentric Chromosomes in Nonstimulated Lymphocyte Prematurely Condensed Chromosomes After Telomere and Centromere Staining. International Journal of Radiation Oncology*Biology*Physics 2015, 91, 640 -649.
AMA StyleRadhia M’Kachera, Elie El Maalouf, Georgia Terzoudi, Michelle Ricoul, Leonhard Heidingsfelder, Ionna Karachristou, Eric Laplagne, William M. Hempel, Bruno Colicchio, Alain Dieterlen, Gabriel Pantelias, Laure Sabatier. Detection and Automated Scoring of Dicentric Chromosomes in Nonstimulated Lymphocyte Prematurely Condensed Chromosomes After Telomere and Centromere Staining. International Journal of Radiation Oncology*Biology*Physics. 2015; 91 (3):640-649.
Chicago/Turabian StyleRadhia M’Kachera; Elie El Maalouf; Georgia Terzoudi; Michelle Ricoul; Leonhard Heidingsfelder; Ionna Karachristou; Eric Laplagne; William M. Hempel; Bruno Colicchio; Alain Dieterlen; Gabriel Pantelias; Laure Sabatier. 2015. "Detection and Automated Scoring of Dicentric Chromosomes in Nonstimulated Lymphocyte Prematurely Condensed Chromosomes After Telomere and Centromere Staining." International Journal of Radiation Oncology*Biology*Physics 91, no. 3: 640-649.
International audienceThe dicentric chromosome (dicentric) assay is the international gold-standard method for bio-logical dosimetry and classification of genotoxic agents. The introduction of telomere and centromere(TC) staining offers the potential to render dicentric scoring more efficient and robust. In this study,we improved the detection of dicentrics and all unstable chromosomal aberrations (CA) leading to asignificant reevaluation of the dose–effect curve and developed an automated approach following TCstaining
Radhia M’Kacher; Elie E.L. Maalouf; Michelle Ricoul; Leonhard Heidingsfelder; Eric Laplagne; Corina Cuceu; William M. Hempel; Bruno Colicchio; Alain Dieterlen; Laure Sabatier. New tool for biological dosimetry: Reevaluation and automation of the gold standard method following telomere and centromere staining. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 2014, 770, 45 -53.
AMA StyleRadhia M’Kacher, Elie E.L. Maalouf, Michelle Ricoul, Leonhard Heidingsfelder, Eric Laplagne, Corina Cuceu, William M. Hempel, Bruno Colicchio, Alain Dieterlen, Laure Sabatier. New tool for biological dosimetry: Reevaluation and automation of the gold standard method following telomere and centromere staining. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. 2014; 770 ():45-53.
Chicago/Turabian StyleRadhia M’Kacher; Elie E.L. Maalouf; Michelle Ricoul; Leonhard Heidingsfelder; Eric Laplagne; Corina Cuceu; William M. Hempel; Bruno Colicchio; Alain Dieterlen; Laure Sabatier. 2014. "New tool for biological dosimetry: Reevaluation and automation of the gold standard method following telomere and centromere staining." Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 770, no. : 45-53.
In three-dimensional fluorescence microscopy the point spread function (PSF) changes with depth, inducing errors in the restored images when these variations are neglected during the deconvolution of thick specimens. Some deconvolution algorithms have been developed to take the depth variations of the PSF into consideration. For these algorithms, the accuracy of the estimated structures depends on the knowledge of the PSF at various depths. We propose an alternative to measuring all required PSFs at different depths. The needed PSFs are interpolated from a limited measured PSF set using a method based on Zernike moments. The proposed method offers the possibility to obtain an accurate PSF interpolation at different depths using only a few measured ones.
Elie Maalouf; Bruno Colicchio; Alain Dieterlen. Fluorescence microscopy three-dimensional depth variant point spread function interpolation using Zernike moments. Journal of the Optical Society of America A 2011, 28, 1864 -1870.
AMA StyleElie Maalouf, Bruno Colicchio, Alain Dieterlen. Fluorescence microscopy three-dimensional depth variant point spread function interpolation using Zernike moments. Journal of the Optical Society of America A. 2011; 28 (9):1864-1870.
Chicago/Turabian StyleElie Maalouf; Bruno Colicchio; Alain Dieterlen. 2011. "Fluorescence microscopy three-dimensional depth variant point spread function interpolation using Zernike moments." Journal of the Optical Society of America A 28, no. 9: 1864-1870.