The nutritional value and flavor and texture characteristics of fruits from different Actinidia argute resources were scientifically evaluated and compared. Using 35 A. arguta fruits as materials, the amino acid composition and content were determined by an automatic amino acid analyzer, and differentiation analysis, amino acid nutritional value evaluation, TAV flavor analysis, correlation analysis, PCA comprehensive evaluation, and cluster analysis were conducted to clarify the diversity of A. arguta resources in terms of amino acid content, composition, and flavor characteristics. Analysis of differential results showed that the A. arguta resource fruits contained 17 amino acids with a total amino acid content of 384.202590.56mg/100g. The results of the nutritional value evaluation showed that the Leu of the fruits of the A. arguta resources all conformed to the ideal model proposed by FAO/WHO, and the Leu content of all the resources exceeded the human bodys needs, and it was also found that the first limiting amino acid of the Actinidia argute resources was Ile and the second limiting amino acid was Lys. TAV of the flavor-presenting amino acids was calculated to evaluate the flavor-presenting taste characteristics, and the amino acids that influenced the flavor of A. arguta fruit were Glu and Cys. PCA showed that the 2 principal components could better reflect the comprehensive information of amino acids in A. arguta, and the cumulative variance contribution rate was 87.88, which could represent the main trend of amino acids in A. arguta. A comprehensive amino acid evaluation model was established, and the composite scores indicated that the top 5 excellent resources were S4, S10, S18, S25, and S30. Hierarchical cluster analysis classified the 35 A. arguta resources into 4 categories, which better reflected the differences in amino acid content and composition, nutritional value, and taste characteristics among A. arguta fruits from different collection sites.

In 2019, bacterial canker caused by Pseudomonas syringae pv. actinidiae was first identified in Actinidia arguta. This disease has led to significant yield reduction, plant mortality, and substantial economic losses in A. arguta cultivation. Its emergence poses a novel challenge to the sustainable global production of kiwifruit. Currently available treatments for bacterial canker caused by P. syringae pv. actinidiae are scarce. Moreover, the environmental toxicity of copper-based compounds and emerging antibiotic resistance issues necessitate the development of eco-friendly control strategies. Disease management strategies based on biocontrol bacteria have shown broad application prospects. In this study, the isolate CHHM-1 with significant antagonistic activity against P. syringae pv. actinidiae was isolated from the rhizosphere soil of healthy A. arguta. It was identified as Pseudomonas koreensis through 16S rRNA gene and whole-genome sequencing. Genomic analysis revealed that the isolate CHHM-1 harbors various genes related to biocontrol, plant growth promotion, and antibiotic resistance, suggesting strong environmental adaptability and functional potential. Furthermore, the strain exhibited multiple plant growth-promoting traits, such as nitrogen fixation, phosphate solubilization, siderophore production, and synthesis of indole-3-acetic acid (IAA). In vitro antagonism assays confirmed the strong antagonistic activity of the isolate CHHM-1 against P. syringae pv. actinidiae. A dual-culture plate assay showed an average inhibition zone of 4.36 cm, while preventive application on plants significantly reduced lesion length to 1.3 mm (vs. 6.2 mm control) in shoots and lesion area to 10% (vs. 80% control) in leaf discs. Further antibacterial tests revealed that its inhibitory mechanism is attributed to secreted antimicrobial substances. These findings provide a promising candidate for developing novel biopesticides to combat P. syringae pv. actinidiae variants, reduce chemical dependency, and foster sustainable A. arguta production.