Aims: Liraglutide is a long-acting glucagon-like peptide 1 (GLP-1) receptor agonist used as an anti-hyperglycemic agent in type 2 diabetes treatment and recently approved for obesity management. Weight loss is attributed to appetite suppression, but therapy may also increase energy expenditure. To further investigate the effect of GLP-1 signaling in thermogenic fat, we assessed adipose tissue oxygen consumption and type 2 deiodinase (D2) activity in mice treated with liraglutide, both basally and after β3-adrenergic treatment.Methods: Male C57BL/6J mice were randomly assigned to receive liraglutide (400 μg/kg, n=12) or vehicle (n=12). After 16 days, mice in each group were co-treated with the selective β3-adrenergic agonist CL316,243 (1 mg/kg, n=6) or vehicle (n=6) for 5 days. Adipose tissue depots were assessed for gene and protein expression, oxygen consumption, and D2 activity.Results: Liraglutide increased interscapular brown adipose tissue (iBAT) oxygen consumption and enhanced β3-adrenergic-induced oxygen consumption in iBAT and inguinal white adipose tissue (ingWAT). These effects were accompanied by upregulation of UCP-1 protein levels in iBAT and ingWAT. Notably, liraglutide increased D2 activity without significantly upregulating its mRNA levels in iBAT and exhibited additive effects to β3-adrenergic stimulation in inducing D2 activity in ingWAT.Conclusions: Liraglutide exhibits additive effects to those of β3-adrenergic stimulation in thermogenic fat and increases D2 activity in BAT, implying that it may activate this adipose tissue depot by increasing intracellular thyroid activation, adding to the currently known mechanisms of GLP-1A-induced weight loss.

Thermogenic adipocytes present a promising therapeutic strategy for metabolic diseases. While murine models have provided valuable insights into thermogenic adipose tissue, their relevance to human physiology is constrained by species-specific differences in tissue distribution and thermogenic capacity. In vitro human models offer a more controlled platform to study adipocyte differentiation, addressing challenges such as limited access to deep fat depots and individual variability. This systematic review summarizes the current literature on human in vitro models for thermogenic adipocyte induction, encompassing 117 studies involving primary human adipocyte progenitors differentiated into thermogenic adipocytes in 2D cultures. Most studies relied on classical adipogenic inducers, including isomethylbutylxanthine, dexamethasone, and insulin, with additional use of triiodothyronine, rosiglitazone, or indomethacin. A few studies incorporated adrenergic stimulation or exposure to lower temperatures to simulate cold exposure. Notably, some studies demonstrated successful differentiation under serum-free, chemically defined conditions, highlighting their potential for reproducibility and translational relevance. A key limitation remains the predominant reliance on gene expression as the primary outcome, with few studies assessing mitochondrial respiration or broader metabolic functions. Moving forward, the development and adoption of standardized, functionally validated protocols will be critical to fully realize the potential of human in vitro thermogenic adipocyte models in metabolic research.

Plantain (Musa paradisiaca) peel is an agro-industrial waste product with remarkable functional potential, attributed to its composition of bioactive compounds with antioxidant and antimicrobial properties. Given this scenario, this scoping review aimed to map and synthesize the scientific evidence regarding the nutritional composition and potential functionalities of plantain peel. A scoping review approach was used, and data were reported using the PRISMA-ScR checklist. The studies evaluating the use of plantain peel were included without restrictions on language or publication date. The following databases were searched: Embase, MEDLINE (via PubMed), Scopus, and Web of Science. Additional searches were conducted through Google Scholar. The protocol has been registered prospectively on the Open Science Framework. This review’s findings included 53 studies. All of them presented methodological limitations that hindered further analysis and the generation of robust evidence. This analysis detailed the chemical composition of the peel, showing that it varies with ripeness stage and processing and is an excellent source of fiber and minerals. Several technological applications are explored, including the use of peel in the production of functional foods, the development of nanoparticles with antimicrobial activity, and its use as a substrate for the biosynthesis of industrial enzymes and citric acid. This review also addresses the possible health benefits that have already been studied in animal and in vitro models. Plantain peel is a promising agro-industrial by-product with high fiber, starch, and bioactive compound content and functional properties. Despite advances, challenges in sensory acceptance and process standardization limit industrial application. A key research gap remains in the systematic evaluation of antinutrient reduction (e.g., oxalates, phytates) and pesticide residue levels during the processing of plantain peel, a mandatory step before its widespread application in the food industry (e.g., flours and food additives). Further research on optimization and bioactive mechanisms is essential to enable its large-scale use and strengthen its role in the circular bioeconomy and human health.

Objetivo: Analisar a frequência e os fatores associados ao risco nutricional (RN) de pacientes com doença renal crônica (DRC) submetidos à hemodiálise. Métodos: Estudo de corte transversal, analítico, realizado com 110 indivíduos com idade ≥ 20 anos,em tratamento hemodialítico em duas clínicas de Cuiabá, Mato Grosso, Brasil, no período de junho a outubro de 2018. Os dados foram coletados por meio de entrevista, aferições e consulta ao prontuário clínico. Avaliou-se a associação entre o desfecho pontuação do Malnutrition Inflammation Score (MIS) e as variáveis socioeconômicas, demográficas, clínicas, de comportamentos de risco relacionados à saúde e de estado nutricional. Realizou-se análise bivariada pelo teste de qui-quadrado de Pearson e regressão logística binária bruta e ajustada, considerando significativo p≤0,05. Resultados: Foi observada elevada frequência de indivíduos com alto RN (n=73; 66,36%), sendo maior entre os pacientes com tempo de diálise ≥ 4 anos, volume de urina/24h