The oral cavity, a dynamic ecosystem, provides an ideal habitat for diverse bacterial species, among which Streptococcus mutans emerges as a key contributor to tooth decay, particularly affecting individuals with temporary dental crowns. This study aimed to replicate oral cavity conditions relevant to those with temporary dental materials, investigating diverse parameters to unveil the optimal environment for S. mutans growth and biofilm formation on polystyrene plates. S. mutans were cultured under various conditions, including the addition of monosaccharides, disaccharides, or trisaccharides, under static or shaking conditions with or without oxygen. This study contributes valuable insights into understanding the intricate dynamics of S. mutans within oral environments, providing a foundation for targeted approaches in dental care and preventive strategies. Our findings demonstrated that the presence of sugars in culture medium supported the development of S. mutans. Notably, cultivating S. mutans in the presence of 2% sucrose under anaerobic conditions with mobility simulation fosters robust bacterial growth and biofilm formation on polystyrene plates.The results underscore the significance of creating experimental conditions that closely mimic the oral environment, enabling researchers to unravel essential aspects of bacterial behavior and enhance our understanding of effective strategies for oral health maintenance. The identified optimal conditions, specifically the influence of sucrose under anaerobic conditions, offer potential avenues for developing preventive interventions in dental care, with implications for those with temporary dental materials.

Background and Objectives: Candida albicans as pathogenic fungi cause conditions like oral candidiasis and dental caries. The critical role of biofilms in the pathogenicity of C. albicans necessitates the exploration of conditions that promote their growth and development. Our study aimed to delineate the optimal conditions conducive to the proliferation and biofilm production of C. albicans on prevalent dental materials. Materials and Methods: To approximate oral cavity conditions, culture media were enhanced with various glucose concen- trations to assess the growth and biofilm-forming capability of the fungus through growth curve analysis and crystal violet assays. Results: The findings suggest that YPG medium augmented with 4% glucose presents as an optimal environment for C. albicans growth. Biofilm formation was most effectively promoted in RPMI medium supplemented with the same concen- tration of glucose. Composite resin was identified as the substrate most susceptible to biofilm development by C. albicans under these conditions. Conclusion: This investigation highlights the necessity of accounting for microbial activity and material characteristics in the prevention and management of dental biofilm formation. Our research advances the understanding of in vitro cultivation of C. albicans, simulating the oral milieu more accurately and contributing to enhanced oral health management for individ- uals utilizing temporary dental fixtures.

Background: Nanomaterials have emerged as a transformative approach in modern pharmaceutical applications, offering advanced benefits compared to conventional therapies. Among available pharmaceutical nanomaterials, silver nanoparticles (AgNPs) have been reported with broad-spectrum antimicrobial potential and drug delivery potency. Nevertheless, some studies suggested that chemical synthesis of AgNPs might result in redundant chemicals, posing environmental and health risks. To minimize undesired products, a promising approach is to biologically synthesize this potent nanomaterial. Methods: This study ultilized an eco-friendly system for AgNPs synthesis using Aspergillus terreus isolated from the air. Physical properties of biosynthesized AgNPs were evaluated by UV–visible spectroscopy, dynamic light scattering, and scanning electron microscopy analysis. Antibacterial activity of biosynthesized AgNPs was examined by well diffusion and minimum inhibitory concentration, while in vitro cytotoxicity was used to determine the antitumor activity of AgNPs. Results: The biosynthesized AgNPs had a size of around 60 nm, a PDI inferior to 0.2, and a zeta potential of −30 mV. They exhibited potent antibacterial activity against both Gram-positive and Gram-negative pathogens. Additionally, these nanoparticles also exerted a selective antiproliferative effect on MCF-7, A549, and MDA-MB-231 cell lines. Conclusions: Our research presented the potential of biosynthesized AgNPs using Aspergillus terreus for antimicrobial and anticancer applications, offering an eco-friendly and sustainable alternative to traditional chemical methods.

The rapid emergence of multi-drug resistant (MDR) bacteria has become a major health concern, driving the need to identify new antimicrobial resources. Recently, endophytes, inhabiting in internal tissues of medicinal plants, have drew important interest from the scientific community, as reservoirs of bioactive metabolites. Numerous studies highlight the symbiotic relationship between plants and their endophytes, in which these microorganisms produce antimicrobial compounds, helping the host plant’s defense against pathogens. Plantago major (commonly known as plantain) is widely recognized for its therapeutic properties, especially for its antimicrobial properties. In this study, endophytic fungi were isolated from Plantago major, morphologically characterized and identified using ITS sequencing. Their antibacterial activity was assessed using the agar diffusion assay. In total, 21 endophytic fungal isolates were obtained from different plant tissues, including leaves, stems, roots, and flowers. Antibacterial assays against methicillin-resistant Staphylococcus aureus (MRSA) were investigated on PDA, SDA, and CDA media. Amongst the isolates, nine strains (MD-H1, MD-L1, MD-L2, MD-L3, MD-L4, MD-L5, MD-R1, MD-T1, MD-T2, and MD-T10) showed medium to strong antibacterial effects, with inhibition zones exceeding 15 mm. The result suggests that endophytic fungi associated with Plantago is a valuable source of anti-MRSA compounds. Further work will focus on identifying the secondary metabolites responsible for this activity and elucidating their chemical structures, providing a basis for the development of new potent antibiotic agents.