In this study, the differences in gene expression of Stropharia rugosoannulata at different treatment times under high temperature and drought stress were analyzed by transcriptomics. Here, a total of 74,571 transcripts and 16,233 unigenes were identified, with an average assembly length of 3002 bp. A total of 10,248 differentially expressed genes (DEGs) were identified. DEG analysis indicated that the numbers of DEGs under high-temperature stress for 1 d, 2 d, and 3 d were 798, 851, and 1484, respectively. These DEGs were involved in 96 GO functional categories and 69 KEGG metabolic pathways. Meanwhile, the numbers of DEGs under drought stress for 3 d, 6 d, and 9 d were 421, 1072, and 2880, respectively. These DEGs were involved in 108 GO functional categories and 78 KEGG metabolic pathways. Further analysis of the metabolic pathway (ko04011) commonly enriched by DEGs identified 15 candidate genes responding to high-temperature or drought stress. Eight candidate genes were randomly selected for qRT-PCR verification, and the qRT-PCR results were basically consistent with the transcriptome datasets. These findings provide critical candidate genes for understanding the molecular regulation mechanism of S. rugosoannulata in response to high temperature and drought stress and have important reference value for its stress resistance breeding.

Light is a crucial environmental regulator for Tricholoma giganteum (T. giganteum). This study investigated the effects of light quality and photoperiod on its growth, physiology, and nutritional composition. During the mycelial stage, blue light (BL) exposure for 5 d promoted the highest growth rate (0.74 mm d⁻¹, 45% higher than dark control, p < 0.05). Red light (RL) enhanced antioxidant capacity, elevating superoxide dismutase (SOD) activity to 240.20 U·mL⁻¹ (after 5 d) and DPPH radical-scavenging activity to 276.11% (after 3 d). Ultraviolet (UV) suppressed polyphenol oxidase (PPO) activity. BL also increased mycelial polysaccharide content (6.45 g·100 g⁻¹). In the fruiting stage, green light (GL) improved agronomic traits and first-grade yield (3.75 kg), while also promoting the accumulation of glutamate (4.39 g·100 g⁻¹), a key umami compound. Further photoperiod optimization revealed that 4 h of daily GL exposure shortened the fruiting cycle, achieved the highest biological efficiency (98.4%), and maximized both polysaccharide (38.17 g·100 g⁻¹) and glutamate contents (5.70 g·100 g⁻¹). These results recommend a two-stage lighting protocol: BL for mycelial growth and a 4 h daily GL for fruiting, providing a scientific basis for the industrial cultivation of T. giganteum.