Abaloparatide is a second-generation osteoporosis drug that prevents bone loss and stimulates bone fromation. This study evaluated the effects of abaloparatide treatment on alveolar bone loss (ABL) in osteoporotic rats with periodontitis by immunohistochemical and microcomputed tomography (micro-CT) in rats with periodontitis and osteoporosis. Forty adult female rats were divided into five equal groups: non-ligated (controls), periodontitis (P), periodontitis + osteoporosis (PO), PO treatment with 10 µg/kg abaloparatide (PO-10), and PO treatment with 80 µg/kg abaloparatide (PO-80). Bilateral ovariectomy was conducted in the osteoporosis groups. Three weeks after ovariectomy, experimental periodontitis was induced via Ligature. Abaloparatide was performed at the same time as the induction of experimental periodontitis. All rats were sacrificed at 30 days. ABL was determined via micro-CT and histological analyses. Bone morphogenetic protein 2 (BMP-2), alkaline phosphatase (ALP), receptor ligand for nuclear factor-kappa B (RANKL), osteocalcin (OCN), osteoprotegerin (OPG), and collagen 1 (Col-1) were evaluated immunohistochemically. In addition, the levels of C-terminal telopeptide of type I collagen (CTX), which is related to bone turnover, were analyzed. Periodontitis with osteoporosis significantly increased ABL in the PO versus the P group. Abaloparatide treatment significantly reduced ABL in the treatment groups and significantly increased BMP-2, ALP, Col-1, OPG, OCN, and CTX levels. In particular, 80 µg/kg abaloparatide treatment significantly modulated the RANKL, OPG, and CTX levels in the PO-80 group. The micro-CT results indicated that abaloparatide treatment significantly enhanced the percentage of bone, trabecular thickness, trabecular number, and bone surface density. Abaloparatide treatment regulates bone formation dose-dependently by decreasing ABL in rats with periodontitis and osteoporosis.

Aim: There is a growing evidence that periodontal disease is associated with an increased risk of cardiovascular diseases. To assess the awareness levels, views, and clinical practices of cardiologists and cardiovascular surgeons in Turkey on the association of periodontal diseases with the diseases of the cardiovascular system. Material and Methods: A survey that included twenty-three questions was prepared and mailed to 99 cardiologists and cardiovascular surgeons practicing as resident, lecturer or specialist in Turkey. The response rate is 66%. Data was analyzed using SPSS ® 18.0 software (SPSS, Chicago, IL, USA). Results: Of the respondents; 80% were males, 98% were at the age of ≤ 50 years, 59% have been working in a university, and 37% were specialists. Fifty-three percent of lecturer and 38% of specialist received a periodontal treatment more than two years time ago. Fourteen percent of resident, 30% of specialist and 53% of lecturer referred patients to visit the dentist. Eighty-eight percent of the residents and lecturers agreed or strongly agreed with the opinion that periodontal disease treatment could lower the risk of an individual to develop a type of cardiovascular disease. Conclusion: Cardiologists and cardiovascular surgeons had a lack of knowledge of the periodontal disease and its impact on systemic health. Programs on oral health in academic curricula of cardiologists and cardiovascular surgeons should be improved to leverage the level of awareness and knowledge on periodontal diseases. Keywords: Cardiologists, cardiovasvular surgeons, cardiovascular diseases, periodontal diseases, oral health. Kardiyologların ve Kardiyovasküler Cerrahların Periodontal Hastalık ve Kardiyovasküler Hastalık Arasındaki İlişkisi ile İlgili Farkındalık, Görüş ve Uygulama Davranışları Amaç: Periodontal hastalığın artmış kardiyovasküler hastalık riski ile ilişkili olduğuna dair kanıtlar artmaktadır. Bu çalışmanın amacı kardiyologların ve kardiyovasküler cerrahların periodontal hastalık ve kardiyovasküler hastalık arasındaki ilişki ile ilgili farkındalık, görüş ve uygulama alışkanlıklarını değerlendirmektir. Gereç ve yöntem: Yirmi üç sorudan oluşan anket hazırlanmış ve Türkiye’de uzman, öğretim üyesi veya asistan olarak çalışan 99 kardiyolog ve kardiyovasküler cerraha mail yoluyla gönderilmiştir. Anketin cevaplanma oranı %66’dır. Veriler SPSS ® 18.0 yazılımı (SPSS, Chicago, IL, USA) kullanılarak analiz edilmiştir. Bulgular: Katılımcıların % 80'i erkek ve % 98'i 50 yaşında veya daha gençtir. Katılımcıların %59’u üniversitede çalışıyor ve %37'si uzman olarak görev yapmaktadır. Öğretim elemanının %53’ü ve uzmanların % 38'i iki yıldan uzun zaman önce periodontal tedavi görmüştür. Asistanların %14’ü, uzmanların % 30'u ve öğretim elemanının % 53'ü hastalarını diş hekimine gitmesi için sevk etmektedir. Asistanların ve öğretim elemanlarının %88’i periodontal hastalığın tedavisinin hastanın kardiyovasküler hastalık riskini azaltabileceği konusuna katılmakta veya çok güçlü katılmaktadır. Sonuç: Kardiyologlar ve kardiyovasküler cerrahlar periodontal hastalık ve periodontal hastalığın sistemik sağlık üzerindeki etkisi hakkında yetersiz bilgiye sahiptir. Kardiyolog ve kalp damar cerrahların akademik müfredatlarında ağız diş sağlığı üzerine programlar, periodontal hastalıklar hakkındaki farkındalık ve bilgi seviyesini arttırmak için geliştirilmelidir. Anahtar kelimeler: Kardiyologlar, kardiovasküler cerrahlar, cardiovascular diseases, periodontal hastalıklar, ağız sağlığı

Periodontitis is a chronic inflammatory disease characterized by progressive destruction of tooth-supporting tissues, primarily driven by a dysregulated host immune response to subgingival biofilms. Toll-like receptors (TLR-2 and TLR-4) and tumor necrosis factor-alpha (TNF-α) are key mediators of immune signaling and tissue breakdown, making them potential biomarkers of periodontal disease activity. This prospective, non-randomized interventional study evaluated TLR-2, TLR-4, and TNF-α levels in gingival crevicular fluid (GCF) for periodontal disease monitoring and assessed the effects of nonsurgical periodontal therapy (NSPT) on clinical parameters. Forty systemically healthy individuals were enrolled and assigned to either periodontitis or healthy groups. Clinical indices—gingival index, plaque index, clinical attachment loss, and probing pocket depth—were recorded at baseline and 8 weeks after NSPT. GCF samples were analyzed using enzyme-linked immunosorbent assay to determine biomarker concentrations. At baseline, TNF-α and TLR-2 levels were significantly higher in periodontitis patients compared with healthy controls (p < 0.001). Following therapy, marked clinical improvements were observed, accompanied by significant reductions in TNF-α and TLR-4 levels (p < 0.05), while TLR-2 levels remained stable. receiver operating characteristic analysis demonstrated that TNF-α and TLR-2 reliably distinguished disease from health. In conclusion, NSPT effectively improved clinical outcomes and reduced TNF-α and TLR-4 levels in GCF, reflecting a decreased local inflammatory burden. The persistence of TLR-2 expression post-treatment suggests a stable role in immune surveillance rather than acute inflammatory response.

Objective: The purpose of this study was to present a bibliometric analysis of scientific articles on clear aligner treatment (CAT) on periodontal health. Materials and Methods: Papers published through July 5, 2023, were searched on the Web of Science (WOS). From a total of 63 papers, the top 25 most cited and appropriate for the study topic were selected. Results: The most cited article with 82 citations was the article published in the European Journal of Orthodontics in 2015. The American Journal of Orthodontics and Dentofacial Orthopedics had the highest publication rate, with three of the top 25 articles published. The first author of the first article was Gabriele Rossini, among the top 25 highly cited articles evaluating the effect of CAT on periodontal health. Italy stands out as the country with the highest number of first authors with 9 articles, followed by China with 7 authors. Conclusions: The bibliometric analysis provides significant information regarding the total number of publications and citations on clear aligner treatment on periodontal health. This bibliometric analysis provides a perspective on the progress of research on clear aligner treatment on periodontal health.

Purpose: The objective of this research was to assess the quality, substance, and ease of reading of online information regarding the use of 3D printing in dental practices. Materials and Methods: The search term selected was ‘3D printing in dentistry’. The first 100 websites retrieved through the Google Search Engine based on this search term were reviewed. Duplicate websites, scientific articles, social media links, videos, advertisements, and broken links were excluded from our study. The quality of the websites analyzed in this study was assessed using the DISCERN tool and JAMA benchmarks, while their readability was measured using the Flesch Reading Ease Score (FRES) and the Flesch-Kincaid Grade Level (FKGL). A significance level of p < 0.05 was established for the analysis. Results: The 75 websites reviewed were categorized into two groups: blogs and commercial sites. It was determined that commercial sites (N: 42) were the majority compared to blog websites (N: 33). Blog websites scored higher than commercial sites in the total DISCERN, DISCERN 1, and Q16 scores. In the JAMA criteria, the minimum sources criterion (n = 12) and the maximum authorship criterion (n = 71) were met. There was no statistically significant difference between the groups in terms of FRES and FKGL scores (p > 0.05). Conclusions: The quality of online information sources regarding ‘3D printers in dentistry’ worldwide is considered to be at a moderate level. The readability of the data is at a low level. There is a need for higher quality and highly readable websites about ‘3D printing in dentistry’.