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Callicarpa candicans (Burm. f.) Hochr. (Callicarpa cana L.) is a medicinal plant that is distributed mainly in the tropics and subtropics of Asia and finds a wide range of uses in traditional medicine. In this study, we attempted and optimized the microwave-assisted hydro-distillation (MAHD) process to obtain essential oil from the leaves of C. candicans. In addition, the obtained oil was analyzed for volatile composition by gas chromatography–mass spectrometry (GC-MS) and assayed for bioactivity against several bacteria and cancer cell lines. To optimize the extraction process, response surface methodology (RSM) in combination with central composite design (CCD) was adopted. Experimental design and optimization were carried out with respect to three experimental factors including the ratio of water to raw material, extraction time, and microwave power. The optimal extraction conditions were obtained as follows: water to raw material ratio of 6/1 (v/w), extraction time 42 min, and microwave power 440 W. Composition determination of the obtained C. candicans essential oil indicated the presence of predominant components including caryophyllene (10.45%), cadinene (10.28%), gurjunene (8.95%), muurolene (8.92%), selinene (7.06%), selinene (5.59%), and copaene (5.40%). In comparison with the essential oils obtained via traditional hydro-distillation method, the essential oil extracted by MAHD exhibited superior anti-proliferative activity on all tested cancer cell lines. Current results imply that the MAHD is capable of recovering biologically-active natural products of greater quantity than that recovered by the conventional distillation.
Quoc Toan Tran; Thu Le Vu Thi; Tien Lam Do; Hong Minh Pham Thi; Bich Hoang Thi; Quang Truyen Chu; Phuong Thao Lai Phuong; Huu Nghi Do; Hoai Thu Hoang Than; Van Huyen Luu; Phuong Thi Mai Duong; Huong Thi Thu Phung; Thu Thuy Ta Thi; Vu Thi; Do; Pham Thi; Hoang Thi; Ta Thi. Optimization of Microwave-Assisted Extraction Process of Callicarpa candicans (Burm. f.) Hochr Essential Oil and Its Inhibitory Properties against Some Bacteria and Cancer Cell Lines. Processes 2020, 8, 173 .
AMA StyleQuoc Toan Tran, Thu Le Vu Thi, Tien Lam Do, Hong Minh Pham Thi, Bich Hoang Thi, Quang Truyen Chu, Phuong Thao Lai Phuong, Huu Nghi Do, Hoai Thu Hoang Than, Van Huyen Luu, Phuong Thi Mai Duong, Huong Thi Thu Phung, Thu Thuy Ta Thi, Vu Thi, Do, Pham Thi, Hoang Thi, Ta Thi. Optimization of Microwave-Assisted Extraction Process of Callicarpa candicans (Burm. f.) Hochr Essential Oil and Its Inhibitory Properties against Some Bacteria and Cancer Cell Lines. Processes. 2020; 8 (2):173.
Chicago/Turabian StyleQuoc Toan Tran; Thu Le Vu Thi; Tien Lam Do; Hong Minh Pham Thi; Bich Hoang Thi; Quang Truyen Chu; Phuong Thao Lai Phuong; Huu Nghi Do; Hoai Thu Hoang Than; Van Huyen Luu; Phuong Thi Mai Duong; Huong Thi Thu Phung; Thu Thuy Ta Thi; Vu Thi; Do; Pham Thi; Hoang Thi; Ta Thi. 2020. "Optimization of Microwave-Assisted Extraction Process of Callicarpa candicans (Burm. f.) Hochr Essential Oil and Its Inhibitory Properties against Some Bacteria and Cancer Cell Lines." Processes 8, no. 2: 173.
Soybeans offer an abundant source of isoflavones, which confer useful bioactivities when existing in aglycone forms. The conversion of isoflavones into aglycones via fermentation of soybean products is often realized by β-glucosidase, an enzyme produced by fungi. In this study, a filamentous fungus, Clerodendron cyrtophyllum, was isolated from root of Clerodendron cyrtophyllum Turcz, which was able to produce the highest activity of β-glucosidase up to 33.72 U/mL at 144 h during fermentation on Potato Dextrose Broth (PDB). The obtained fungus was grown on isoflavones-rich soybean extract to produce genistein and daidzein, achieving the conversion rate of 98.7%. Genistein and daidzein were isolated and purified by column chromatography using hexane/acetone (29:1/1:1), reaching purities of over 90% of total isoflavones, as identified and determined by TLC, LC-MS/MS, and 1H and 13C NMR spectroscopy. These results imply that the isolated P. citrinum is a potential fungal strain for industrial-scale production of genistein and daidzein from isoflavones-containing soybean extracts. These products may serve as potential raw materials for manufacture of functional foods that are based on aglycones.
Duy Tien Doan; Duc Phuong Luu; Thanh Duong Nguyen; Bich Hoang Thi; Hong Minh Pham Thi; Huu Nghi Do; Van Huyen Luu; The Dan Pham; Van Thai Than; Hai Ha Pham Thi; Minh Quan Pham; Quoc Toan Tran. Isolation of Penicillium citrinum from Roots of Clerodendron cyrtophyllum and Application in Biosynthesis of Aglycone Isoflavones from Soybean Waste Fermentation. Foods 2019, 8, 554 .
AMA StyleDuy Tien Doan, Duc Phuong Luu, Thanh Duong Nguyen, Bich Hoang Thi, Hong Minh Pham Thi, Huu Nghi Do, Van Huyen Luu, The Dan Pham, Van Thai Than, Hai Ha Pham Thi, Minh Quan Pham, Quoc Toan Tran. Isolation of Penicillium citrinum from Roots of Clerodendron cyrtophyllum and Application in Biosynthesis of Aglycone Isoflavones from Soybean Waste Fermentation. Foods. 2019; 8 (11):554.
Chicago/Turabian StyleDuy Tien Doan; Duc Phuong Luu; Thanh Duong Nguyen; Bich Hoang Thi; Hong Minh Pham Thi; Huu Nghi Do; Van Huyen Luu; The Dan Pham; Van Thai Than; Hai Ha Pham Thi; Minh Quan Pham; Quoc Toan Tran. 2019. "Isolation of Penicillium citrinum from Roots of Clerodendron cyrtophyllum and Application in Biosynthesis of Aglycone Isoflavones from Soybean Waste Fermentation." Foods 8, no. 11: 554.
Alpha-glucosidase inhibitory activity has been commonly used for the evaluation of antidiabetic property in vitro. The aim of this study is to investigate and characterize Dalbergia tonkinensis as a potential source of antidiabetic compounds. The screening of the active parts used, such as trunk bark, heartwood, and the leaves of Dalbergia tonkinensis indicated that all these extracted parts used with methanol demonstrated potent α-glucosidase inhibitory activity. The in vitro antidiabetic property of Dalbergia tonkinensis was notably recorded for the first time and showed activity (EC50 = 0.17–0.78 mg/mL) comparable to those of reported potent herbal extracts (EC50 = 0.25–4.0 mg/mL) and higher activity than that of acarbose, a commercial antidiabetic drug (EC50 = 1.21 mg/mL). The stability tests revealed that the heartwood of Dalbergia tonkinensis extract (HDT) possesses high pH stability with relative activity in the range of 80–98%. Further bioassay-guided purification led to the isolation of 2 active compounds identified as sativanone and formononetin from the ethyl acetate fraction and water fraction of HDT, respectively. These α-glucosidase inhibitors (aGIs) show promising inhibition against various types of α-glucosidases. Remarkably, these inhibitors were determined as new mammalian aGIs, showing good effect on rat α-glucosidase. The results suggest that Dalbergia tonkinensis is a potent source of aGIs and suggest promise in being developed as functional food with antidiabetic efficacy. The results of this study also enrich our knowledge concerning current biological activity and constituents of Dalbergia tonkinensis species.
Van Bon Nguyen; San-Lang Wang; Ngu Truong Nhan; Thi Hanh Nguyen; Nguyen Phuong Dai Nguyen; Do Huu Nghi; Nguyen Manh Cuong. New Records of Potent In-Vitro Antidiabetic Properties of Dalbergia tonkinensis Heartwood and the Bioactivity-Guided Isolation of Active Compounds. Molecules 2018, 23, 1589 .
AMA StyleVan Bon Nguyen, San-Lang Wang, Ngu Truong Nhan, Thi Hanh Nguyen, Nguyen Phuong Dai Nguyen, Do Huu Nghi, Nguyen Manh Cuong. New Records of Potent In-Vitro Antidiabetic Properties of Dalbergia tonkinensis Heartwood and the Bioactivity-Guided Isolation of Active Compounds. Molecules. 2018; 23 (7):1589.
Chicago/Turabian StyleVan Bon Nguyen; San-Lang Wang; Ngu Truong Nhan; Thi Hanh Nguyen; Nguyen Phuong Dai Nguyen; Do Huu Nghi; Nguyen Manh Cuong. 2018. "New Records of Potent In-Vitro Antidiabetic Properties of Dalbergia tonkinensis Heartwood and the Bioactivity-Guided Isolation of Active Compounds." Molecules 23, no. 7: 1589.