Tillering and panicle branching are important determinants of plant architecture and yield potential in rice (Oryza sativa). IDEAL PLANT ARCHITECTURE1 (IPA1) encodesSQUAMOSA PROMOTER BINDING PROTEIN-LIKE14, which acts as a key transcription factor regulating tiller outgrowth and panicle branching by directly activating the expression of O. sativa TEOSINTE BRANCHED1 (OsTB1) and O. sativa DENSEAND ERECT PANICLE1 (OsDEP1), thereby influencing grain yield in rice. Here, we report the identification of a rice mutant named shi1 that is characterized by dramatically reduced tiller number, enhanced culm strength, and increased panicle branch number. Map-based cloning revealed that O. sativa SHORT INTERNODES1 (OsSHI1) encodes a plant-specific transcription factor of the SHI family with a characteristic family-specific IGGH domain and a conserved zinc-finger DNA binding domain. Consistent with the mutant phenotype, OsSHI1 is predominantly expressed in axillary buds and young panicle, and its encoded protein is exclusively targeted to the nucleus. We show that OsSHI1 physically interacts with IPA1 both in vitro and in vivo. Moreover, OsSHI1 could bind directly to the promoter regions of both OsTB1 and OsDEP1 through a previously unrecognized cis-element (T/GCTCTAC motif). OsSHI1 repressed the transcriptional activation activity of IPA1 by affecting its DNA binding activity toward the promoters of both OsTB1 and OsDEP1, resulting in increased tiller number and diminished panicle size. Taken together, our results demonstrate that OsSHI1 regulates plant architecture through modulating the transcriptional activity of IPA1 and provide insight into the establishment of plant architecture in rice.

GOT1B regulates COPII-mediated protein export from the endoplasmic reticulum exit sites in developing rice endosperm cells.

Seed-setting rate is a critical determinant of grain yield in rice (Oryza sativa L.). Rapid and healthy pollen tube growth in the style is required for high seed-setting rate. The molecular mechanisms governing this process remain largely unknown. In this study, we isolate a dominant low seed-setting rate rice mutant, sss1-D. Cellular examination results show that pollen tube growth is blocked in about half of the mutant styles. Molecular cloning and functional assays reveals that SSS1-D encodes OsCNGC13, a member of the cyclic nucleotide-gated channel family. OsCNGC13 is preferentially expressed in the pistils and its expression is dramatically reduced in the heterozygous plant, suggesting a haploinsufficiency nature for the dominant mutant phenotype. We show that OsCNGC13 is permeable to Ca²⁺. Consistent with this, accumulation of cytoplasmic calcium concentration ([Ca²⁺]_cyt) is defective in the sss1-D mutant style after pollination. Further, the sss1-D mutant has altered extracellular matrix (ECM) components and delayed cell death in the style transmission tract (STT). Based on these results, we propose that OsCNGC13 acts as a novel maternal sporophytic factor required for stylar [Ca²⁺]_cyt accumulation, ECM components modification and STT cell death, thus facilitating the penetration of pollen tube in the style for successful double fertilization and seed-setting in rice. Rice is not only the staple food for more than half of the world’s population, but also a model species for plant developmental and genetic studies. After pollination, rice pollen grains adhere and hydrate at the surface of stigmatic papilla cells. Then, the germinated pollen tubes invade the stigma and navigate through the style transmission tract to reach the micropyle of the embryo sac for fertilization. During this long and arduous process, pollen tube requires abundant communication with the surrounding sporophytic maternal tissues. However, how the growth of pollen tube is regulated by maternal tissue remains largely elusive. This work identifies a typical cyclic nucleotide-gated channel protein in rice, OsCNGC13, which can mediate Ca²⁺ inward current. Our results suggest that OsCNGC13 acts as a novel maternal sporophytic factor required for stylar [Ca²⁺]_cyt accumulation, extracellular matrix components modification and style cell death, thus facilitating the penetration of pollen tube in the style for successful double fertilization and seed-setting in rice. These findings provide new insights into the molecular genetic control mechanisms of seed-setting rate/grain yield in rice and expand our knowledge on the cyclic nucleotide-gated channel proteins in plant sexual reproduction.

The White Panicle1 gene, encoding a Val-tRNA synthetase, plays an essential role in early chloroplast development.