Maize is a vital cereal crop enhancing food security. Optimizing plant density is a common practice to increase crop yield. This study investigates the impacts of high plant density on the biochemical composition, dry matter accumulation (DMA), photosynthesis-active radiation (PAR), radiation use efficiency (RUE), grain production, and stalk lodging in maize cultivation. Five plant densities (4.5, 6, 7.5, 9, and 15 plants m-2) referred to as PD4.5, PD6, PD7.5, PD9, and PD15, respectively, were tested on three maize hybrids, Zhengdan 958 (ZD958), Longping 206 (LP206), and Jinqiu 119 (JQ119), at the Mengcheng Research Station during 2016 and 2017. High plant densities increased PAR, RUE, DMA, and grain yield and led to a higher incidence of stalk lodging. The highest grain yields were achieved at PD6 (9.44%) and PD7.5 (2.98%), while yields decreased at PD15 (12.5%) due to a 60% increase in lodging. Under high plant density, internodes experienced rapid accumulation of carbohydrates but ceased elongating, expanding, and developing structural carbohydrates. Stem bending resistance positively correlated with cellulose, hemicellulose, and lignin content (0.9293**, 0.8572**, 0.8976**), as did stem crushing resistance with the same components (0.9096**, 0.8372**, 0.8738**). The present study’s outcome will help determine the optimum plant density to improve food security and enhance maize yield under different climatic conditions.