Microalgae are used as dietary supplements for humans and animals, due to their excellent nutritional profile. This research aims to characterize Spirugrass^®, a co-product obtained after the extraction of phycocyanin from Limnospira platensis, and to evaluate whether a stabilization treatment involving high-pressure processing (HPP) affects its proteomic profile. The research also aims to evaluate the possible use of Spirugrass^® as feed integration for honeybee health. Proteins were extracted and fractionated using size exclusion chromatography (SEC) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The iron content was measured using atomic absorption spectrometry (AAS). Samples of Spirugrass^® were subjected to HPP at 600 MPa for five minutes and the effect on the integrity of the protein profile was analyzed. Finally, nine groups of 30 newly emerged honeybees were supplemented with Spirugrass^® in a controlled laboratory experiment. SDS-PAGE and SEC enabled the assessment of the quality and integrity of the Spirugrass^® proteome, which contains 80% of the proteins found in the algal biomass, including phycocyanin. The phycocyanin purity indices were 2.07 ± 0.14 and 0.72 ± 0.13 for the L. platensis and Spirugrass^® extracts, respectively. Spirugrass^® maintains a consistent iron content of 261 ± 15 μg/g dry weight, equivalent to 74% of the iron present in the algal biomass. In both L. platensis and Spirugrass^®, iron was predominantly bound to high-molecular-mass proteins, including phycocyanin. Following HPP treatment, differences in the protein profiles were observed, which suggests partial protein degradation. Preliminary data obtained with honeybees are encouraging, as no mortality or adverse effects were observed and Spirugrass^® can be considered a promising candidate as feed supplement. Overall, the presence of consistent levels of soluble proteins, as well as protein-bound iron, suggests that Spirugrass^® could be used in animal nutrition.

Sea turtles are increasingly being used as bioindicators of marine pollution, yet baseline data on trace elements in the blood are still limited. This study quantified magnesium (Mg), iron (Fe), zinc (Zn), copper (Cu), cadmium (Cd) and lead (Pb) in green turtles (Chelonia mydas) (55 plasma samples and 71 cell concentrate samples) and olive ridleys (Lepidochelys olivacea) (101 plasma samples and 65 cell concentrate samples) sampled off the Caribbean (Tortuguero) and Pacific (Ostional) coasts of Costa Rica in 2003–2004. The metals were measured using atomic absorption spectroscopy; whole-blood concentrations were derived from the plasma and the erythrocyte values. The present results were compared with published datasets to evaluate the spatial and temporal patterns of metal exposure over the past two decades. The essential elements showed matrix-specific distributions, with Mg and Cu higher in the plasma, and Fe and Zn higher in the cell concentrates in both species (p < 0.001). C. mydas generally exhibited higher Cu, Fe and Zn levels in the plasma (p < 0.001), whereas L. olivacea showed markedly higher Cd levels (p < 0.001). Overall, the Pb levels were low as compared with many other rookeries worldwide. These data provide one of the earliest, large-sample baselines for trace elements in sea turtle blood in the Eastern Tropical Pacific and Western Caribbean and underscore the value of blood-fraction analysis for long-term ecotoxicological monitoring.

Background: Urinary proteins may reflect physiological changes occurring during the periparturient period, but reference data for goats are still lacking. This study investigated urinary protein patterns around parturition to help fill this gap and generate baseline information. Methods: Ten pregnant Alpine goats were sampled by spontaneous voiding 22 ± 3 days before delivery (T0), 7 days postpartum (T7), and 30 days postpartum (T30). Physical and chemical urine analyses were performed, and urinary proteins were separated using one-dimensional sodium dodecyl sulfate–polyacrylamide gel electrophoresis. Statistical tests (Shapiro–Wilk, repeated-measures ANOVA, or Friedman) were applied to evaluate differences among time points. Results: Significant temporal changes were observed: urine pH decreased at T30, the urine protein-to-creatinine ratio increased at T7 and T30, and urinary creatinine concentration was highest at T0. Most samples showed common protein bands at approximately 80, 70, 62, 50, 37, 29, 25, 22, and below 13 kDa, with the 62, 50, and <13 kDa bands present in all samples. Bands between 18 and 64 kDa and above 60 kDa appeared only in some samples. Protein bands between 23 and 42 kDa were more frequent at T0, suggesting immune-related variations associated with pregnancy. Conclusions: This study provides the first description of urinary protein electrophoretic profiles in goats during the periparturient period and highlights measurable changes across time. These findings offer a starting point for developing future research and may contribute to establishing reference parameters for clinical and physiological monitoring in goats.