Abdelmageed M. Othman

Prof. Dr. Abdelmageed M. Othman

National Research Center, Giza, Al Jīzah, Egypt
orcid

Research Focus & Metrics

Academic Career Length

18Years (2009-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

Representative Publications

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1 November 2019 in Journal of Genetic Engineering and Biotechnology
Verified
Journal Article
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Abdelmageed M. OthmanAbdelmageed M. Othman
Abdelmageed M. Othman
MEMaysa A. Elsayed
Maysa A. Elsayed
NANaser Gad Al-Balakocy
Naser Gad Al-Balakocy
MHMohamed M. Hassan
Mohamed M. Hassan
AEAli M. Elshafei
Ali M. Elshafei
The present study aims to apply an efficient eco-friendly and inexpensive process for green synthesis of silver nanoparticles (AgNPs) through the mediation of fungal proteins from Aspergillus fumigatus DSM819, characterization, and its application as antimicrobial finishing agent in textile fabrics against some infectious microorganisms. Optimum conditions for AgNP biosynthesis could be achieved by means of using 60% (v/v) of cell-free filtrate (CFF) and 1.5 mM of AgNO3 at pH 10.0 after 90 min. The obtained AgNPs were of spherical shape with 90% of distribution below than 84.4 nm. The biosynthesized AgNPs exerted an antimicrobial activity against the studied pathogenic microorganisms (E. coli, B. mycoides, and C. albicans). In addition, IC50 values against in vitro tumor cell lines were found to be 31.1, 45.4, 40.9, and 33.5 μg/ml for HCT116, A549, MCF7, and PC3, respectively. Even with a very low concentration (0.25%), the treated PET/C fabrics by AgNPs exerted an antimicrobial activity against E. coli, B. mycoides, and C. albicans to give inhibition zone diameter of 15, 15, and 16 mm, respectively. The green biosynthesis approach applied in this study is a non-toxic alternative to the traditional chemical and physical methods, and would be appropriate for biological large-scale production and prospective treatments.

2 January 2023 in Microbial Cell Factories
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Journal Article
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AEAlshaimaa M. Elsayed
Alshaimaa M. Elsayed
MMMohamed Mahmoud
Mohamed Mahmoud
GKGhada S. A. Abdel Karim
Ghada S. A. Abdel Karim
MAMohamed Abdelraof
Mohamed Abdelraof
Abdelmageed M. OthmanAbdelmageed M. Othman
Abdelmageed M. Othman
Two laccase isoenzymes (LacA and LacB) were isolated from a novelTrichoderma harzianumS7113 isolate employing ammonium sulfate precipitation, Sephadex G100, and DEAE Sepharose ion exchange chromatography. The molecular weights of the purified LacA and LacB laccases were estimated to be 63 and 48 kDa, respectively. The two isoenzymes had their optimum activities at the same temperature (50 °C), but at slightly different pH values (pH 3.0 for LacA and pH 2.5 for LacB). LacA and LacB had the same thermal stability at 40 °C and pH stability at pH 9.0. The two isoenzymes also showed a high level of specific activity toward ABTS, where theKmvalues of LacA and LacB were 0.100 and 0.065 mM, whereas theirVmaxvalues were 0.603 and 0.182 µmol min−1, respectively. LacA and LacB catalytic activity was stimulated by Mg2+, Zn2+, K+, and Ni2+, whereas it was inhibited by Hg2+and Pb2+, β-mercaptoethanol, EDTA, and SDS, and completely inhibited by sodium azide. Our findings indicate that purified laccase has a promising capacity for bisphenol A (BPA) bioremediation across a broad pH range. This finding opens up new opportunities for the commercialization of this technique in a variety of biotechnology-based applications, particularly for removing endocrine chemicals from the environment.

1 July 2018 in International Journal of Biological Macromolecules
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Journal Article
Closed access
Abdelmageed M. OthmanAbdelmageed M. Othman
Abdelmageed M. Othman
MEMaysa A. Elsayed
Maysa A. Elsayed
AEAli M. Elshafei
Ali M. Elshafei
MHMohamed M. Hassan
Mohamed M. Hassan

1 September 2019 in International Journal of Biological Macromolecules
Verified
Journal Article
Closed access
SMSalah A. Mohamed
Salah A. Mohamed
MEMohamed El-Sakhawy
Mohamed El-Sakhawy
ENEl-Shahat H.A. Nashy
El-Shahat H.A. Nashy
Abdelmageed M. OthmanAbdelmageed M. Othman
Abdelmageed M. Othman

2 December 2016 in RSC Advances
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Journal Article
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Abdelmageed M. OthmanAbdelmageed M. Othman
Abdelmageed M. Othman
EGElena González-Domínguez
Elena González-Domínguez
M. Ángeles SanrománM. Ángeles Sanromán
M. Ángeles Sanromán
Miguel A. Correa-DuarteMiguel A. Correa-Duarte
Miguel A. Correa-Duarte
Diego MoldesDiego Moldes
Diego Moldes
Myceliophthora thermophila laccase was covalently immobilized on functionalized multiwalled carbon nanotubes (MWNT) arranged over a supporting membrane to obtain a permeable bio-barrier that could be applied in multibatch or continuous processes. Initially, several supporting materials, namely cellulose nitrate, agarose and polyvinyl alcohol, were evaluated as supports for the MWNT. The maximum enzyme loading of 0.286 U mg−1 of MWNT was obtained for cellulose nitrate support supplemented with 0.21 mg MWNT. Immobilized laccase showed good operational stability (above 95% of its initial activity was maintained after 10 cycles of reaction) and exhibited improved stability towards temperature, pH and acetone: for instance, 21% and 49% for free and immobilized laccase, respectively, when incubated in the presence of 20% of acetone (v/v) for 6 h. The immobilized laccase was applied for Reactive Black 5 (RB5) decolorization. Maximum values of RB5 decolorization were observed in the presence of 1-hydroxybenzotriazole as mediator and at pH 5.0, with 68.09 and 84.26% decolorization after 6 and 24 h, respectively.

2 January 2019 in Applied Physics A
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ANAmany. M. El Nahrawy
Amany. M. El Nahrawy
AHAli B. Abou Hammad
Ali B. Abou Hammad
AYAhmed Youssef
Ahmed Youssef
AMAbdelfattah M Mansour
Abdelfattah M Mansour
Abdelmageed M. OthmanAbdelmageed M. Othman
Abdelmageed M. Othman