Fluorescence anisotropy imaging and sensing is a widely recognized method for studying molecular orientation and mobility. However, introducing this technique to in vivo systems is a challenging task, especially when one considers multiphoton excitation methods. Past two decades have brought a possible solution to this issue in the form of hollow-core antiresonant fibers (HC-ARFs). The continuous development of their fabrication technology has resulted in the appearance of more and more sophisticated structures. One of the most promising concepts concerns dual hollow-core antiresonant fibers (DHC-ARFs), which can be used to split and combine optical signals, effectively working as optical fiber couplers. In this paper, the design of a fluorescence anisotropy sensor based on a DHC-ARF structure is presented. The main purpose of the proposed DHC-ARF is multiphoton-excited fluorescence spectroscopy; however, other applications are also possible.

With the growing interest in hollow-core antiresonant fibers (HC-ARF), attributed to the development of their fabrication technology, the appearance of more sophisticated structures is understandable. One of the recently advancing concepts is that of dual hollow-core antiresonant fibers, which have the potential to be used as optical fiber couplers. In the following paper, a design of a dual hollow-core antiresonant fiber (DHC-ARF) acting as a polarization fiber coupler is presented. The structure is based on a highly birefringent hollow-core fiber design, which is proven to be a promising solution for the purpose of propagation of polarized signals. The design of an optimized DHC-ARF with asymmetrical cores is proposed, together with analysis of its essential coupling parameters, such as the extinction ratio, coupling length ratio, and coupling strength. The latter two for the x- and y-polarized signals were ~2 and 1, respectively, while the optical losses were below 0.3 dB/cm in the 1500–1700 nm transmission band.

Long-period optical fiber gratings (LPGs) are one of the widely used concepts for the sensing of refractive index (RI) changes. Negative curvature hollow-core fibers (NCHCFs), with their relatively large internal diameters that are easy to fill with liquids, appear as a very interesting medium to combine with the idea of LPGs and use for RI sensing. However, to date, there has been no investigation of the RI sensing capabilities of the NCHCF-based LPGs. The results presented in the paper do not only address this matter, but also compare the RI sensitivities of the NCHCFs alone and the gratings. By modeling two revolver-type fibers, with their internal diameters reflecting the results of the possible LPG-inscription process, the authors show that the fibers’ transmission windows shift in response to the RI change, resulting in changes in RI sensitivities as high as −4411 nm/RIU. On the contrary, the shift in the transmission dip of the NCHCF-based LPGs corresponds to a sensitivity of −658 nm/RIU. A general confirmation of these results was ensured by comparing the analytical formulas describing the sensitivities of the NCHCFs and the NCHCF-based LPGs.

In this paper, an application of negative curvature hollow core fiber (NCHCF) in an all-fiber, multiphoton fluorescence sensor setup is presented. The dispersion parameter (D) of this fiber does not exceed the value of 5 ps/nm × km across the optical spectrum of (680–750) nm, making it well suited for the purpose of multiphoton excitation of biological fluorophores. Employing 1.5 m of this fiber in a simple, all-fiber sensor setup allows us to perform multiphoton experiments without any dispersion compensation methods. Multiphoton excitation of nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD) with this fiber shows a 6- and 9-fold increase, respectively, in the total fluorescence signal collected when compared with the commercial solution in the form of a hollow-core photonic band gap fiber (HCPBF). To the author’s best knowledge, this is the first time an NCHCF was used in an optical-fiber sensor setup for multiphoton fluorescence experiments.

In this paper, we present numerical studies of several different structures of anti-resonant, hollow core optical fibers. The cladding of these fibers is based on the Kagomé lattice concept, with some of the core-surrounding lattice cells removed. This modification, by creating additional, glass-free regions around the core, results in a significant improvement of some important optical fiber parameters, such as confinement loss (CL), bending loss (BL), and dispersion parameter (D). According to the conducted simulations (with fused silica glass being the structure’s material), CL were reduced from ~0.36 dB/m to ~0.16 dB/m (at 760 nm wavelength) in case of the structure with removed cells, and did not exceed the value of 1 dB/m across the 700–850 nm wavelength range. Additionally, proposed structure exhibits a remarkably low value of D—from 1.5 to 2.5 ps/(nm × km) at the 700–800 nm wavelength range, while the BL were estimated to be below 0.25 dB/m for bending radius of ~1.5 cm. CL and D were simulated, additionally, for structures made of acrylic glass polymethylmethacrylate, (PMMA), with similarly good results—D_PMMA ∊ [2, 4] ps/(nm × km) and CL_PMMA ≈ 0.13 dB/m (down from 0.41 dB/m), for the same spectral regions (700–800 nm bandwidth for D, and 760 nm wavelength for CL).

The presence of fiber optic devices, such as couplers or wavelength division multiplexers, based on hollow-core fibers (HCFs) is still rather uncommon, while such devices can be imagined to greatly increase the potential of HCFs for different applications, such as sensing, nonlinear optics, etc. In this paper, we present a combination of a standard, multimode fiber (MMF) optic coupler with a hollow core photonic bandgap fiber through arc fusion splicing and its application for the purpose of multiphoton spectroscopy. The presented splicing method is of high affordability due to the low cost of arc fusion splicers, and the measured splicing loss (SL) of the HCF-MMF splice is as low as (0.32 ± 0.1) dB, while the splice itself is durable enough to withstand a bending radius (r_bend) of 1.8 cm. This resulted in a hybrid between the hollow core photonic bandgap fiber (HCPBF) and MMF coupler, delivering 20 mW of average power and 250-fs short laser pulses to the sample, which was good enough to test the proposed sensor setup in a simple, proof-of-concept multiphoton fluorescence excitation-detection experiment, allowing the successful measurement of the fluorescence emission spectrum of 10⁻⁵ M fluorescein solution. In our opinion, the presented results indicate the possibility of creating multi-purpose HCF setups, which would excel in various types of sensing applications.