In this study, a nano-Ca(OH)₂/Fe₃O₄ catalyst was used to produce biodiesel from a 1:1 mixed jatropha-castor oil. By loading Ca(OH)₂ onto Fe₃O₄ nanoparticles, it increased the specific surface area by almost 40%, which improved the catalytic activity as it provided a larger area for the reactants to interact. The main purpose of mixing jatropha oil with castor oil was to lower the viscosity of the castor oil. The transesterification reaction was carried out at elevated temperature, using a microwave heating system. Moreover, it was shown that the preferred reaction conditions are using high temperature and short reaction duration. The optimized yield of methyl ester was 95%, achieved by using a catalyst with a Ca:Fe ratio of 7:1, temperature of 65 °C, methanol/oil ratio of 12:1, and reaction time of 35 min. The catalyst was shown to be reusable, easily recyclable, and its activity was very stable. Only 2% of the catalyst was lost, and the yield was 3% lower after ten successive applications. The solid, magnetic base catalyst could be easily separated from the reaction products, unlike homogeneous catalysts.

Laminar flow microbial fuel cells (MFCs) are used to understand the role of microorganisms, and their interactions with electrodes in microbial bioelectrochemical systems. In this study, we reported the flow characteristics of laminar flow in a typical MFC configuration in a non-dimensional form, which can serve as a guideline in the design of such microfluidic systems. Computational fluid dynamics simulations were performed to examine the effects of channel geometries, surface characteristics, and fluid velocity on the mixing dynamics in microchannels with a rectangular cross-section. The results showed that decreasing the fluid velocity enhances mixing but changing the angle between the inlet channels, only had strong effects when the angle was larger than 135°. Furthermore, different mixing behaviors were observed depending on the angle of the channels, when the microchannel aspect ratio was reduced. Asymmetric growth of microbial biofilm on the anode side skewed the mixing zone and wall roughness due to the bacterial attachment, which accelerated the mixing process and reduced the efficiency of the laminar flow MFC. Finally, the magnitude of mass diffusivity had a substantial effect on mixing behavior. The results shown here provided both design guidelines, as well as better understandings of the MFCs due to microbial growth.