Bovine tuberculosis (bTB) is a disease of cattle that represents a risk to public health and causes severe economic losses to the livestock industry. Recently, one of the strategies recommended for reducing the prevalence of the disease in animals is the use of the BCG vaccine, alone or in combination with proteins. It has been shown that the vaccine elicits a strong immune response, downsizes the number of animals with visible lesions, and reduces the rate of infection as well as the bacillary count. This paper, based on scientific evidence, makes suggestions about some practical vaccination alternatives that can be used in infected herds to reduce bTB prevalence, considering BCG strains, vaccine doses, routes of application, and age of the animals. Our conclusion is that vaccination is a promising alternative to be included in current control programs in underdeveloped countries to reduce the disease burden.

The spatio-temporal epidemiology of rabies has related the influence of environmental factors and anthropogenic changes on the movements of the hematophagous bat Desmodus rotundus. In Mexico, D. rotundus is the main transmitter of the rabies virus for different livestock species, modifying annually the fluctuation of the number of cases of rabies and its dissemination in subtropical areas and regions considered free of the disease. The purpose of this study was to perform a descriptive analysis of the distribution of cases of rabies in Mexico, and to perform a time-series analysis to evaluate stationarity and to predict the number of cases for the following year. A total of 3,469 cases were reported in the period of interest, of which the 89.1% occurred in cattle, 4.3% in horses, 1.5% in sheep, 0.6% in goats, 0.01% in pig, 3.1% in vampire bats, 0.3% in cervids, 0.2% in skunks, 0.1% in insectivorous bats, 0.1% in foxes, 0.1% in buffaloes, and 0.02% in coatis; 0.5% were not identified. The most frequent antigenic variants reported were AgV11, AgV5, and AgV3, associated with D. rotundus. The distribution of cases in bats correlates with the distribution of cases in domestic and wild animals; however, cases were observed in wild species in non-endemic areas of Mexico, like the State of Chihuahua. The additive model used in the time-series analysis showed a seasonal pattern with a peak of cases at the beginning of each year, from January to March. The model showed a good predicting value; the Pearson correlation coefficient R² was 0.705. The highest probability for the occurrence of rabies cases in the different species estimated by Ordinary Kriging was in the coast of the Gulf of Mexico, involving the states of Tamaulipas, Veracruz, Tabasco, Chiapas, and Yucatan. This study confirms that rabies in domestic and wild species is endemic in tropical and subtropical areas—however, cases have been observed in new geographic areas—and provides useful information to support actions to stop the spread of the rabies virus or the reservoir, and for planning vaccination strategies considering time and place.

Giardia is a protozoan that affects humans as well as a wide range of domestic species. It is distributed worldwide, and the highest frequency is seen in developing countries. Due to the potential for domestic cats to be carriers of this parasite and subsequently transmit the infection to humans, it is important to know the risk of transmission. For this reason, the objective of this study was to determine the frequency of this parasite in the cat population of the city of Santiago de Queretaro, Mexico, and identify the assemblages present to determine the role this host plays in public health, this being the first study of its type to be performed in the country. This was a cross-sectional study during which 200 fecal samples were collected from cats of both sexes and varying ages and strata of origin. The samples were analyzed by microscopy following the flotation technique, having obtained a general frequency of 25%. Giardia cysts were found at higher frequency in pasty stools. The assemblages found were zoonotic, specifically assemblage A, which suggests that the cat poses an important risk for the dissemination of the parasite to humans, making it an important public health problem.

Rabies is a zoonotic disease that affects livestock worldwide. The distribution of rabies is highly correlated with the distribution of the vampire bat Desmodus rotundus, the main vector of the disease. In this study, climatic, topographic, livestock population, vampire distribution and urban and rural zones were used to estimate the risk for presentation of cases of rabies in Mexico by co- Kriging interpolation. The highest risk for the presentation of cases is in the endemic areas of the disease, i.e. the States of Yucatán, Chiapas, Campeche, Quintana Roo, Tabasco, Veracruz, San Luis Potosí, Nayarit and Baja California Sur. A transition zone for cases was identified across northern Mexico, involving the States of Sonora, Sinaloa, Chihuahua, and Durango. The variables topography, vampire distribution, bovine population and rural zones are the most important to explain the risk of cases in livestock. This study provides robust estimates of risk and spread of rabies based on geostatistical methods. The information presented should be useful for authorities responsible of public and animal health when they plan and establish strategies preventing the spread of rabies into rabies-free regions of México.

Attempts to improve the immune response and efficacy of vaccines against tuberculosis in cattle, goats, and other animal species have been the focus of research in this field during the last two decades. Improving the vaccine efficacy is essential prior to running long-lasting and expensive field trials. Studies have shown that vaccine protocols utilizing boosting with proteins improve the vaccine efficacy. The use of polymers such as chitosan and PolyLactic-co-Glycolic Acid (PLGA) improves the immune response against different diseases by improving the interaction of antigens with the cellular immune system and modulating the host immune response. This study shows that the prime BCG vaccination, boosted with a culture filtrate protein (CFP), alone or in combination with chitosan and PolyLactic-co-Glycolic Acid (PLGA), have the potential to reduce tuberculosis (TB) dissemination by reducing the number of animals with lesions, the number of lesions per animal, and the size of the lesions in vaccinated animals, compared with those not vaccinated or those vaccinated with BCG alone. The vaccinated groups showed significantly higher Interferon-γ levels in the blood compared to the control, nonvaccinated group after vaccination, after boosting, and after the challenge with the wild-type Mycobacterium bovis strain.

Bats are ecologically vital mammals, serving as pollinators, seed dispersers, and bioindicators of ecosystem health. Many species inhabit natural caves, which offer optimal conditions for survival but present challenges for direct ecological monitoring due to their dark, complex, and inaccessible environments. Traditional monitoring methods, such as mist-netting, are invasive and limited in scope, highlighting the need for non-intrusive alternatives. In this work, we present a portable multisensor platform designed to operate in underground habitats. The system captures multimodal data, including near-infrared (NIR) imagery, ultrasonic audio, 3D structural data, and RGB video. Focusing on NIR imagery, we evaluate the effectiveness of the YOLO object detection framework for automated bat detection and counting. Experiments were conducted using a dataset of NIR images collected in natural shelters. Three YOLO variants (v10, v11, and v12) were trained and tested on this dataset. The models achieved high detection accuracy, with YOLO v12m reaching a mean average precision (mAP) of 0.981. These results demonstrate that combining NIR imaging with deep learning enables accurate and non-invasive monitoring of bats in challenging environments. The proposed approach offers a scalable tool for ecological research and conservation, supporting population assessment and behavioral studies without disturbing bat colonies.